From bulk, single-cell to spatial RNA sequencing

Abstract RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA seque...

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Autores principales:	Xinmin Li, Cun-Yu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Publishing Group 2021
Materias:	Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/83b2ddf152304608adbe69c6ad05caf9
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spelling	oai:doaj.org-article:83b2ddf152304608adbe69c6ad05caf92021-11-21T12:06:35ZFrom bulk, single-cell to spatial RNA sequencing10.1038/s41368-021-00146-01674-28182049-3169https://doaj.org/article/83b2ddf152304608adbe69c6ad05caf92021-11-01T00:00:00Zhttps://doi.org/10.1038/s41368-021-00146-0https://doaj.org/toc/1674-2818https://doaj.org/toc/2049-3169Abstract RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.Xinmin LiCun-Yu WangNature Publishing GrouparticleDentistryRK1-715ENInternational Journal of Oral Science, Vol 13, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dentistry RK1-715
spellingShingle	Dentistry RK1-715 Xinmin Li Cun-Yu Wang From bulk, single-cell to spatial RNA sequencing
description	Abstract RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing. This most widely used technology in genomics tool box has evolved from classic bulk RNA sequencing (RNAseq), popular single cell RNA sequencing (scRNAseq) to newly emerged spatial RNA sequencing (spRNAseq). Bulk RNAseq studies average global gene expression, scRNAseq investigates single cell RNA biology up to 20,000 individual cells simultaneously, while spRNAseq has ability to dissect RNA activities spatially, representing next generation of RNA sequencing. This article highlights these technologies, characteristic features and suitable applications in precision oncology.
format	article
author	Xinmin Li Cun-Yu Wang
author_facet	Xinmin Li Cun-Yu Wang
author_sort	Xinmin Li
title	From bulk, single-cell to spatial RNA sequencing
title_short	From bulk, single-cell to spatial RNA sequencing
title_full	From bulk, single-cell to spatial RNA sequencing
title_fullStr	From bulk, single-cell to spatial RNA sequencing
title_full_unstemmed	From bulk, single-cell to spatial RNA sequencing
title_sort	from bulk, single-cell to spatial rna sequencing
publisher	Nature Publishing Group
publishDate	2021
url	https://doaj.org/article/83b2ddf152304608adbe69c6ad05caf9
work_keys_str_mv	AT xinminli frombulksinglecelltospatialrnasequencing AT cunyuwang frombulksinglecelltospatialrnasequencing
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From bulk, single-cell to spatial RNA sequencing

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