Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat

Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) i...

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Autores principales:	Manuela Zinni, Julien Pansiot, Marina Colella, Valérie Faivre, Andrée Delahaye-Duriez, François Guillonneau, Johanna Bruce, Virginie Salnot, Jérôme Mairesse, Marit Knoop, Marie-Laure Possovre, Daniel Vaiman, Olivier Baud
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microglia IUGR inflammation proteome brain development oxidative stress Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/83b901ca42524446bd52d0b133eac300
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spelling	oai:doaj.org-article:83b901ca42524446bd52d0b133eac3002021-11-25T18:33:26ZImpact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat10.3390/nu131137192072-6643https://doaj.org/article/83b901ca42524446bd52d0b133eac3002021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3719https://doaj.org/toc/2072-6643Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) in rats. Microglia, isolated from control and growth-restricted animals at P1 and P4, showed significant changes in the proteome between the two groups. The expression of protein sets associated with fetal growth, inflammation, and the immune response were significantly enriched in LPD microglia at P1 and P4. Interestingly, upregulation of protein sets associated with the oxidative stress response and reactive oxygen species production was observed at P4 but not P1. During development, inflammation-associated proteins were upregulated between P1 and P4 in both control and LPD microglia. By contrast, proteins associated with DNA repair and senescence pathways were upregulated in only LPD microglia. Similarly, protein sets involved in protein retrograde transport were significantly downregulated in only LPD microglia. Overall, these data demonstrate significant and multiple effects of LPD-induced IUGR on the developmental program of microglial cells, leading to an abnormal proteome within the first postnatal days.Manuela ZinniJulien PansiotMarina ColellaValérie FaivreAndrée Delahaye-DuriezFrançois GuillonneauJohanna BruceVirginie SalnotJérôme MairesseMarit KnoopMarie-Laure PossovreDaniel VaimanOlivier BaudMDPI AGarticlemicrogliaIUGRinflammationproteomebrain developmentoxidative stressNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3719, p 3719 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microglia IUGR inflammation proteome brain development oxidative stress Nutrition. Foods and food supply TX341-641
spellingShingle	microglia IUGR inflammation proteome brain development oxidative stress Nutrition. Foods and food supply TX341-641 Manuela Zinni Julien Pansiot Marina Colella Valérie Faivre Andrée Delahaye-Duriez François Guillonneau Johanna Bruce Virginie Salnot Jérôme Mairesse Marit Knoop Marie-Laure Possovre Daniel Vaiman Olivier Baud Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
description	Microglial activation is a key modulator of brain vulnerability in response to intra-uterine growth restriction (IUGR). However, the consequences of IUGR on microglial development and the microglial proteome are still unknown. We used a model of IUGR induced by a gestational low-protein diet (LPD) in rats. Microglia, isolated from control and growth-restricted animals at P1 and P4, showed significant changes in the proteome between the two groups. The expression of protein sets associated with fetal growth, inflammation, and the immune response were significantly enriched in LPD microglia at P1 and P4. Interestingly, upregulation of protein sets associated with the oxidative stress response and reactive oxygen species production was observed at P4 but not P1. During development, inflammation-associated proteins were upregulated between P1 and P4 in both control and LPD microglia. By contrast, proteins associated with DNA repair and senescence pathways were upregulated in only LPD microglia. Similarly, protein sets involved in protein retrograde transport were significantly downregulated in only LPD microglia. Overall, these data demonstrate significant and multiple effects of LPD-induced IUGR on the developmental program of microglial cells, leading to an abnormal proteome within the first postnatal days.
format	article
author	Manuela Zinni Julien Pansiot Marina Colella Valérie Faivre Andrée Delahaye-Duriez François Guillonneau Johanna Bruce Virginie Salnot Jérôme Mairesse Marit Knoop Marie-Laure Possovre Daniel Vaiman Olivier Baud
author_facet	Manuela Zinni Julien Pansiot Marina Colella Valérie Faivre Andrée Delahaye-Duriez François Guillonneau Johanna Bruce Virginie Salnot Jérôme Mairesse Marit Knoop Marie-Laure Possovre Daniel Vaiman Olivier Baud
author_sort	Manuela Zinni
title	Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
title_short	Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
title_full	Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
title_fullStr	Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
title_full_unstemmed	Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat
title_sort	impact of fetal growth restriction on the neonatal microglial proteome in the rat
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/83b901ca42524446bd52d0b133eac300
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Impact of Fetal Growth Restriction on the Neonatal Microglial Proteome in the Rat

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