Morphological and molecular identification of cellulolytic fungi associated with local raru species

Abstract. Elfiati D, Susilowati A, Modes C, Rachmat HH. 2019. Morphological and molecular identification of cellulolytic fungi associated with local raru species. Biodiversitas 20: 2348-2354. This study is aimed to isolate and identify cellulolytic fungi from different local Raru species in Central...

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Autores principales:	Deni Elfiati, ARIDA SUSILOWATI, CELVIA MODES, HENTI HENDALASTUTI RACHMAT
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cellulolytic fungi, morphology, molecular identification, internal transcribed spacer, aspergillus terreus Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/83bcb6b8bf374ca4a6916c209cf38991
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spelling	oai:doaj.org-article:83bcb6b8bf374ca4a6916c209cf389912021-11-21T21:43:54ZMorphological and molecular identification of cellulolytic fungi associated with local raru species1412-033X2085-472210.13057/biodiv/d200833https://doaj.org/article/83bcb6b8bf374ca4a6916c209cf389912019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4101https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Elfiati D, Susilowati A, Modes C, Rachmat HH. 2019. Morphological and molecular identification of cellulolytic fungi associated with local raru species. Biodiversitas 20: 2348-2354. This study is aimed to isolate and identify cellulolytic fungi from different local Raru species in Central Tapanuli, North Sumatra, Indonesia. Cellulolytic fungi are fungi that can degrade natural polymer materials such as cellulose. The fungal species were isolated from soil under three local raru species. The samples inoculating (1 mL) from serial dilutions (10-2 to 10-5) on Carboxy Methyl Cellulose (CMC) plates. The plates were incubated at 28 ?C for one week, then the fungal colonies were observed and pure cultures were maintained. Fungi with cellulolytic activity were characterized by the formation of clear zones around the colonies on CMC media plus congo red (0.1%). The identification of fungi was carried out by molecular identification of the isolated fungi at the species level. The extracted fungal DNA was amplified by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in Gen Bank (NCBI). 20 isolates were successfully isolated and nine isolates had cellulolytic activity. Six fungal species from 20 isolates were identified as: Aspergillus sp., Penicillium sp., Aspergillus terreus, Penicillium mariae-crucis, Aspergillus tanneri and Penicillium citrinum. The results showed that Aspergillus terreus was the most abundant fungus obtained from all the raru stand.Deni ElfiatiARIDA SUSILOWATICELVIA MODESHENTI HENDALASTUTI RACHMATMBI & UNS Soloarticlecellulolytic fungi, morphology, molecular identification, internal transcribed spacer, aspergillus terreusBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cellulolytic fungi, morphology, molecular identification, internal transcribed spacer, aspergillus terreus Biology (General) QH301-705.5
spellingShingle	cellulolytic fungi, morphology, molecular identification, internal transcribed spacer, aspergillus terreus Biology (General) QH301-705.5 Deni Elfiati ARIDA SUSILOWATI CELVIA MODES HENTI HENDALASTUTI RACHMAT Morphological and molecular identification of cellulolytic fungi associated with local raru species
description	Abstract. Elfiati D, Susilowati A, Modes C, Rachmat HH. 2019. Morphological and molecular identification of cellulolytic fungi associated with local raru species. Biodiversitas 20: 2348-2354. This study is aimed to isolate and identify cellulolytic fungi from different local Raru species in Central Tapanuli, North Sumatra, Indonesia. Cellulolytic fungi are fungi that can degrade natural polymer materials such as cellulose. The fungal species were isolated from soil under three local raru species. The samples inoculating (1 mL) from serial dilutions (10-2 to 10-5) on Carboxy Methyl Cellulose (CMC) plates. The plates were incubated at 28 ?C for one week, then the fungal colonies were observed and pure cultures were maintained. Fungi with cellulolytic activity were characterized by the formation of clear zones around the colonies on CMC media plus congo red (0.1%). The identification of fungi was carried out by molecular identification of the isolated fungi at the species level. The extracted fungal DNA was amplified by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in Gen Bank (NCBI). 20 isolates were successfully isolated and nine isolates had cellulolytic activity. Six fungal species from 20 isolates were identified as: Aspergillus sp., Penicillium sp., Aspergillus terreus, Penicillium mariae-crucis, Aspergillus tanneri and Penicillium citrinum. The results showed that Aspergillus terreus was the most abundant fungus obtained from all the raru stand.
format	article
author	Deni Elfiati ARIDA SUSILOWATI CELVIA MODES HENTI HENDALASTUTI RACHMAT
author_facet	Deni Elfiati ARIDA SUSILOWATI CELVIA MODES HENTI HENDALASTUTI RACHMAT
author_sort	Deni Elfiati
title	Morphological and molecular identification of cellulolytic fungi associated with local raru species
title_short	Morphological and molecular identification of cellulolytic fungi associated with local raru species
title_full	Morphological and molecular identification of cellulolytic fungi associated with local raru species
title_fullStr	Morphological and molecular identification of cellulolytic fungi associated with local raru species
title_full_unstemmed	Morphological and molecular identification of cellulolytic fungi associated with local raru species
title_sort	morphological and molecular identification of cellulolytic fungi associated with local raru species
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/83bcb6b8bf374ca4a6916c209cf38991
work_keys_str_mv	AT denielfiati morphologicalandmolecularidentificationofcellulolyticfungiassociatedwithlocalraruspecies AT aridasusilowati morphologicalandmolecularidentificationofcellulolyticfungiassociatedwithlocalraruspecies AT celviamodes morphologicalandmolecularidentificationofcellulolyticfungiassociatedwithlocalraruspecies AT hentihendalastutirachmat morphologicalandmolecularidentificationofcellulolyticfungiassociatedwithlocalraruspecies
_version_	1718418652249718784

Morphological and molecular identification of cellulolytic fungi associated with local raru species

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