Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition

Abstract The worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rati...

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Autores principales:	Yong He, Hang Zhao, Yuanwen Liu, He Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83c5a6b9708a4c13beb54a3059c20b25
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spelling	oai:doaj.org-article:83c5a6b9708a4c13beb54a3059c20b252021-12-02T16:53:00ZSpecific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition10.1038/s41598-021-90619-32045-2322https://doaj.org/article/83c5a6b9708a4c13beb54a3059c20b252021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90619-3https://doaj.org/toc/2045-2322Abstract The worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rational antibiotic prescription. Here, a recombinant bacteriophage tail fiber protein (TFP) was functionalized on magnetic particles to specifically capture Pseudomonas aeruginosa, while fluorescein isothiocyanate-labeled-magainin II was utilized as the indicator. For solving the magnetic particles’ blocking effects, a reverse assaying protocol based on TFP recognition was developed to investigate the feasibility of detection and AST of P. aeruginosa. P. aeruginosa can be rapidly, sensitively and specifically detected within 1.5 h with a linear range of 1.0 × 102 to 1.0 × 106 colony forming units (CFU)⋅mL−1 and a detection limit of 3.3 × 10 CFU⋅mL−1. Subsequently, AST results, which were consistent with broth dilution results, can be obtained within 3.5 h. Due to the high specificity of the TFP, AST can actually be conducted without the need for bacterial isolation and identification. Based on the proof-of-principle work, the detection and AST of other pathogens can be extended by expressing the TFPs of their bacteriophages.Yong HeHang ZhaoYuanwen LiuHe ZhouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yong He Hang Zhao Yuanwen Liu He Zhou Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
description	Abstract The worldwide emergence and spread of antimicrobial resistance is accelerated by irrational administration and use of empiric antibiotics. A key point to the crisis is a lack of rapid diagnostic protocols for antimicrobial susceptibility testing (AST), which is crucial for a timely and rational antibiotic prescription. Here, a recombinant bacteriophage tail fiber protein (TFP) was functionalized on magnetic particles to specifically capture Pseudomonas aeruginosa, while fluorescein isothiocyanate-labeled-magainin II was utilized as the indicator. For solving the magnetic particles’ blocking effects, a reverse assaying protocol based on TFP recognition was developed to investigate the feasibility of detection and AST of P. aeruginosa. P. aeruginosa can be rapidly, sensitively and specifically detected within 1.5 h with a linear range of 1.0 × 102 to 1.0 × 106 colony forming units (CFU)⋅mL−1 and a detection limit of 3.3 × 10 CFU⋅mL−1. Subsequently, AST results, which were consistent with broth dilution results, can be obtained within 3.5 h. Due to the high specificity of the TFP, AST can actually be conducted without the need for bacterial isolation and identification. Based on the proof-of-principle work, the detection and AST of other pathogens can be extended by expressing the TFPs of their bacteriophages.
format	article
author	Yong He Hang Zhao Yuanwen Liu He Zhou
author_facet	Yong He Hang Zhao Yuanwen Liu He Zhou
author_sort	Yong He
title	Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
title_short	Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
title_full	Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
title_fullStr	Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
title_full_unstemmed	Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition
title_sort	specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of pseudomonas aeruginosa based on dual molecular recognition
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/83c5a6b9708a4c13beb54a3059c20b25
work_keys_str_mv	AT yonghe specificandrapidreverseassayingprotocolfordetectionandantimicrobialsusceptibilitytestingofpseudomonasaeruginosabasedondualmolecularrecognition AT hangzhao specificandrapidreverseassayingprotocolfordetectionandantimicrobialsusceptibilitytestingofpseudomonasaeruginosabasedondualmolecularrecognition AT yuanwenliu specificandrapidreverseassayingprotocolfordetectionandantimicrobialsusceptibilitytestingofpseudomonasaeruginosabasedondualmolecularrecognition AT hezhou specificandrapidreverseassayingprotocolfordetectionandantimicrobialsusceptibilitytestingofpseudomonasaeruginosabasedondualmolecularrecognition
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Specific and rapid reverse assaying protocol for detection and antimicrobial susceptibility testing of Pseudomonas aeruginosa based on dual molecular recognition

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