Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.

<h4>Background</h4>Dengue (DEN) is a neglected tropical disease, and surveillance of dengue virus (DENV) serotypes and genotypes is critical for the early detection of outbreaks. Risk factors for outbreaks include the emergence of new genotypes and serotype shifting.<h4>Methodology...

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Autores principales:	Mya Myat Ngwe Tun, Aung Kyaw Kyaw, Takeshi Nabeshima, Aung Min Soe, Khine Mya Nwe, Kyaw Ko Ko Htet, Thet Htoo Aung, Thein Thein Htwe, Thidar Aung, Su Su Myaing, Tu Tu Mar, Ei Phyu Lwin, Hlaing Myat Thu, Kyaw Zin Thant, Kouichi Morita
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83c69ed152cb485db99cda2b18c130ff
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spelling	oai:doaj.org-article:83c69ed152cb485db99cda2b18c130ff2021-12-02T20:03:55ZDetection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.1932-620310.1371/journal.pone.0251314https://doaj.org/article/83c69ed152cb485db99cda2b18c130ff2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251314https://doaj.org/toc/1932-6203<h4>Background</h4>Dengue (DEN) is a neglected tropical disease, and surveillance of dengue virus (DENV) serotypes and genotypes is critical for the early detection of outbreaks. Risk factors for outbreaks include the emergence of new genotypes and serotype shifting.<h4>Methodology and principal findings</h4>To understand the genomic and viral characteristics of DENV-infected patients, we conducted a cross-sectional descriptive study among pediatric patients admitted at the 550-bedded Mandalay Children Hospital during the 2018 DEN endemic season. We conducted virus isolation, serological tests, viremia level measurement, and whole-genome sequencing. Among the 202 serum samples, we detected 85 samples with DENV (46 DENV-1, 10 DENV-3, 26 DENV-4 and three multiple serotype co-infections) via reverse transcription quantitative/real-time PCR (RT-qPCR), and we obtained 49 DENV isolates (31 DENV-1, 10 DENV-3 and 8 DEN-4). We did not detect DENV-2 in this study. The viral genome levels in serum did not differ significantly among virus serotypes, infection status (primary versus secondary) and disease severity. Based on the phylogenetic analysis, we identified DENV-1 genotype-1, DENV-4 genotype-1 and DENV-3 genotype-3 and genotype-1 which was detected for the first time. Next-generation sequencing analysis revealed greater frequencies of nonsynonymous and synonymous mutations per gene in the nonstructural genes. Moreover, mutation rates were also higher among DENV-1.<h4>Conclusion/significance</h4>In conclusion, there was an increasing trend of DENV-3 cases during DENV endemic season in 2018 with the first detection of the genotype 1. However, DENV-1 has remained the predominant serotype in this study area since 2013, and we identified stop codon mutations in the DENV-1 genome. This report is the first to feature a complete genome analysis of the strains of DENV-3 and DENV-4 circulating among pediatric patients in Myanmar. This study highlighted the importance of annual surveillance for a better understanding of the molecular epidemiology of DENVs.Mya Myat Ngwe TunAung Kyaw KyawTakeshi NabeshimaAung Min SoeKhine Mya NweKyaw Ko Ko HtetThet Htoo AungThein Thein HtweThidar AungSu Su MyaingTu Tu MarEi Phyu LwinHlaing Myat ThuKyaw Zin ThantKouichi MoritaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0251314 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mya Myat Ngwe Tun Aung Kyaw Kyaw Takeshi Nabeshima Aung Min Soe Khine Mya Nwe Kyaw Ko Ko Htet Thet Htoo Aung Thein Thein Htwe Thidar Aung Su Su Myaing Tu Tu Mar Ei Phyu Lwin Hlaing Myat Thu Kyaw Zin Thant Kouichi Morita Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
description	<h4>Background</h4>Dengue (DEN) is a neglected tropical disease, and surveillance of dengue virus (DENV) serotypes and genotypes is critical for the early detection of outbreaks. Risk factors for outbreaks include the emergence of new genotypes and serotype shifting.<h4>Methodology and principal findings</h4>To understand the genomic and viral characteristics of DENV-infected patients, we conducted a cross-sectional descriptive study among pediatric patients admitted at the 550-bedded Mandalay Children Hospital during the 2018 DEN endemic season. We conducted virus isolation, serological tests, viremia level measurement, and whole-genome sequencing. Among the 202 serum samples, we detected 85 samples with DENV (46 DENV-1, 10 DENV-3, 26 DENV-4 and three multiple serotype co-infections) via reverse transcription quantitative/real-time PCR (RT-qPCR), and we obtained 49 DENV isolates (31 DENV-1, 10 DENV-3 and 8 DEN-4). We did not detect DENV-2 in this study. The viral genome levels in serum did not differ significantly among virus serotypes, infection status (primary versus secondary) and disease severity. Based on the phylogenetic analysis, we identified DENV-1 genotype-1, DENV-4 genotype-1 and DENV-3 genotype-3 and genotype-1 which was detected for the first time. Next-generation sequencing analysis revealed greater frequencies of nonsynonymous and synonymous mutations per gene in the nonstructural genes. Moreover, mutation rates were also higher among DENV-1.<h4>Conclusion/significance</h4>In conclusion, there was an increasing trend of DENV-3 cases during DENV endemic season in 2018 with the first detection of the genotype 1. However, DENV-1 has remained the predominant serotype in this study area since 2013, and we identified stop codon mutations in the DENV-1 genome. This report is the first to feature a complete genome analysis of the strains of DENV-3 and DENV-4 circulating among pediatric patients in Myanmar. This study highlighted the importance of annual surveillance for a better understanding of the molecular epidemiology of DENVs.
format	article
author	Mya Myat Ngwe Tun Aung Kyaw Kyaw Takeshi Nabeshima Aung Min Soe Khine Mya Nwe Kyaw Ko Ko Htet Thet Htoo Aung Thein Thein Htwe Thidar Aung Su Su Myaing Tu Tu Mar Ei Phyu Lwin Hlaing Myat Thu Kyaw Zin Thant Kouichi Morita
author_facet	Mya Myat Ngwe Tun Aung Kyaw Kyaw Takeshi Nabeshima Aung Min Soe Khine Mya Nwe Kyaw Ko Ko Htet Thet Htoo Aung Thein Thein Htwe Thidar Aung Su Su Myaing Tu Tu Mar Ei Phyu Lwin Hlaing Myat Thu Kyaw Zin Thant Kouichi Morita
author_sort	Mya Myat Ngwe Tun
title	Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
title_short	Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
title_full	Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
title_fullStr	Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
title_full_unstemmed	Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.
title_sort	detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in mandalay, upper myanmar.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/83c69ed152cb485db99cda2b18c130ff
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Detection of genotype-1 of dengue virus serotype 3 for the first time and complete genome analysis of dengue viruses during the 2018 epidemic in Mandalay, Upper Myanmar.

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