A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.

To better understand the olfactory mechanisms in a lepidopteran pest model species, the cotton leafworm Spodoptera littoralis, we have recently established a partial transcriptome from adult antennae. Here, we completed this transcriptome using next generation sequencing technologies, namely 454 and...

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Autores principales:	Erwan Poivet, Aurore Gallot, Nicolas Montagné, Nicolas Glaser, Fabrice Legeai, Emmanuelle Jacquin-Joly
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83c9615d7b3243c4b7cc8f46abf67d5b
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spelling	oai:doaj.org-article:83c9615d7b3243c4b7cc8f46abf67d5b2021-11-18T07:51:01ZA comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.1932-620310.1371/journal.pone.0060263https://doaj.org/article/83c9615d7b3243c4b7cc8f46abf67d5b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23565215/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To better understand the olfactory mechanisms in a lepidopteran pest model species, the cotton leafworm Spodoptera littoralis, we have recently established a partial transcriptome from adult antennae. Here, we completed this transcriptome using next generation sequencing technologies, namely 454 and Illumina, on both adult antennae and larval tissues, including caterpillar antennae and maxillary palps. All sequences were assembled in 77,643 contigs. Their analysis greatly enriched the repertoire of chemosensory genes in this species, with a total of 57 candidate odorant-binding and chemosensory proteins, 47 olfactory receptors, 6 gustatory receptors and 17 ionotropic receptors. Using RT-PCR, we conducted the first exhaustive comparison of olfactory gene expression between larvae and adults in a lepidopteran species. All the 127 candidate olfactory genes were profiled for expression in male and female adult antennae and in caterpillar antennae and maxillary palps. We found that caterpillars expressed a smaller set of olfactory genes than adults, with a large overlap between these two developmental stages. Two binding proteins appeared to be larvae-specific and two others were adult-specific. Interestingly, comparison between caterpillar antennae and maxillary palps revealed numerous organ-specific transcripts, suggesting the complementary involvement of these two organs in larval chemosensory detection. Adult males and females shared the same set of olfactory transcripts, except two male-specific candidate pheromone receptors, two male-specific and two female-specific odorant-binding proteins. This study identified transcripts that may be important for sex-specific or developmental stage-specific chemosensory behaviors.Erwan PoivetAurore GallotNicolas MontagnéNicolas GlaserFabrice LegeaiEmmanuelle Jacquin-JolyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e60263 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erwan Poivet Aurore Gallot Nicolas Montagné Nicolas Glaser Fabrice Legeai Emmanuelle Jacquin-Joly A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
description	To better understand the olfactory mechanisms in a lepidopteran pest model species, the cotton leafworm Spodoptera littoralis, we have recently established a partial transcriptome from adult antennae. Here, we completed this transcriptome using next generation sequencing technologies, namely 454 and Illumina, on both adult antennae and larval tissues, including caterpillar antennae and maxillary palps. All sequences were assembled in 77,643 contigs. Their analysis greatly enriched the repertoire of chemosensory genes in this species, with a total of 57 candidate odorant-binding and chemosensory proteins, 47 olfactory receptors, 6 gustatory receptors and 17 ionotropic receptors. Using RT-PCR, we conducted the first exhaustive comparison of olfactory gene expression between larvae and adults in a lepidopteran species. All the 127 candidate olfactory genes were profiled for expression in male and female adult antennae and in caterpillar antennae and maxillary palps. We found that caterpillars expressed a smaller set of olfactory genes than adults, with a large overlap between these two developmental stages. Two binding proteins appeared to be larvae-specific and two others were adult-specific. Interestingly, comparison between caterpillar antennae and maxillary palps revealed numerous organ-specific transcripts, suggesting the complementary involvement of these two organs in larval chemosensory detection. Adult males and females shared the same set of olfactory transcripts, except two male-specific candidate pheromone receptors, two male-specific and two female-specific odorant-binding proteins. This study identified transcripts that may be important for sex-specific or developmental stage-specific chemosensory behaviors.
format	article
author	Erwan Poivet Aurore Gallot Nicolas Montagné Nicolas Glaser Fabrice Legeai Emmanuelle Jacquin-Joly
author_facet	Erwan Poivet Aurore Gallot Nicolas Montagné Nicolas Glaser Fabrice Legeai Emmanuelle Jacquin-Joly
author_sort	Erwan Poivet
title	A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
title_short	A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
title_full	A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
title_fullStr	A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
title_full_unstemmed	A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.
title_sort	comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth spodoptera littoralis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/83c9615d7b3243c4b7cc8f46abf67d5b
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A comparison of the olfactory gene repertoires of adults and larvae in the noctuid moth Spodoptera littoralis.

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