Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

Abstract The silkworm Bombyx mori is a poikilotherm and is therefore sensitive to various climatic conditions. The influence of temperature on the intestinal flora and the relationship between the intestinal flora and gene expression in the silkworm remain unknown. In the present study, changes of t...

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Autores principales:	Zhenli Sun, Dhiraj Kumar, Guangli Cao, Liyuan Zhu, Bo Liu, Min Zhu, Zi Liang, Sulan Kuang, Fei Chen, Yongjie Feng, Xiaolong Hu, Renyu Xue, Chengliang Gong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83cdb51fd6dc4c209985ee72a9395943
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spelling	oai:doaj.org-article:83cdb51fd6dc4c209985ee72a93959432021-12-02T15:05:41ZEffects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori10.1038/s41598-017-03565-42045-2322https://doaj.org/article/83cdb51fd6dc4c209985ee72a93959432017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03565-4https://doaj.org/toc/2045-2322Abstract The silkworm Bombyx mori is a poikilotherm and is therefore sensitive to various climatic conditions. The influence of temperature on the intestinal flora and the relationship between the intestinal flora and gene expression in the silkworm remain unknown. In the present study, changes of the intestinal flora at 48, 96 and 144 h following transient high temperature treatment (THTT) of 37 °C for 8 h were investigated. According to principal component analysis, the abundances of Enterococcus and Staphylococcus showed a negative correlation with other dominant genera. After THTT, the gene expression levels of spatzle-1 and dicer-2 were increased and decreased, respectively, which suggested that the Toll and RNAi pathways were activated and suppressed, respectively. The species-gene expression matrix confirmed that the spatzle-1 and dicer-2 gene expression levels were negatively and positively correlated, respectively, with the abundance of Enterococcus and Staphylococcus in the control. The abundance of Variovorax post-THTT was positively correlated with the spatzle-1 gene expression level, whereas the community richness of Enterococcus was negatively correlated with the spatzle-1 gene expression level and positively correlated with the dicer-2. The results of the present investigation provide new evidence for understanding the relationships among THTT, intestinal flora and host gene expression.Zhenli SunDhiraj KumarGuangli CaoLiyuan ZhuBo LiuMin ZhuZi LiangSulan KuangFei ChenYongjie FengXiaolong HuRenyu XueChengliang GongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhenli Sun Dhiraj Kumar Guangli Cao Liyuan Zhu Bo Liu Min Zhu Zi Liang Sulan Kuang Fei Chen Yongjie Feng Xiaolong Hu Renyu Xue Chengliang Gong Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
description	Abstract The silkworm Bombyx mori is a poikilotherm and is therefore sensitive to various climatic conditions. The influence of temperature on the intestinal flora and the relationship between the intestinal flora and gene expression in the silkworm remain unknown. In the present study, changes of the intestinal flora at 48, 96 and 144 h following transient high temperature treatment (THTT) of 37 °C for 8 h were investigated. According to principal component analysis, the abundances of Enterococcus and Staphylococcus showed a negative correlation with other dominant genera. After THTT, the gene expression levels of spatzle-1 and dicer-2 were increased and decreased, respectively, which suggested that the Toll and RNAi pathways were activated and suppressed, respectively. The species-gene expression matrix confirmed that the spatzle-1 and dicer-2 gene expression levels were negatively and positively correlated, respectively, with the abundance of Enterococcus and Staphylococcus in the control. The abundance of Variovorax post-THTT was positively correlated with the spatzle-1 gene expression level, whereas the community richness of Enterococcus was negatively correlated with the spatzle-1 gene expression level and positively correlated with the dicer-2. The results of the present investigation provide new evidence for understanding the relationships among THTT, intestinal flora and host gene expression.
format	article
author	Zhenli Sun Dhiraj Kumar Guangli Cao Liyuan Zhu Bo Liu Min Zhu Zi Liang Sulan Kuang Fei Chen Yongjie Feng Xiaolong Hu Renyu Xue Chengliang Gong
author_facet	Zhenli Sun Dhiraj Kumar Guangli Cao Liyuan Zhu Bo Liu Min Zhu Zi Liang Sulan Kuang Fei Chen Yongjie Feng Xiaolong Hu Renyu Xue Chengliang Gong
author_sort	Zhenli Sun
title	Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
title_short	Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
title_full	Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
title_fullStr	Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
title_full_unstemmed	Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori
title_sort	effects of transient high temperature treatment on the intestinal flora of the silkworm bombyx mori
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/83cdb51fd6dc4c209985ee72a9395943
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Effects of transient high temperature treatment on the intestinal flora of the silkworm Bombyx mori

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