An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.

<h4>Background</h4>Histone tails have a plethora of different post-translational modifications, which are located differently in "open" and "closed" parts of genomes. H3K4me3/H3K79me2 and H4K20me3 are among the histone marks associated with the early establishment of...

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Autores principales:	Giacomo Donati, Raffaella Gatta, Diletta Dolfini, Andrea Fossati, Michele Ceribelli, Roberto Mantovani
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83d09b13b14e4f1688bb4ee883744ab3
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spelling	oai:doaj.org-article:83d09b13b14e4f1688bb4ee883744ab32021-11-25T06:12:42ZAn NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.1932-620310.1371/journal.pone.0002066https://doaj.org/article/83d09b13b14e4f1688bb4ee883744ab32008-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18446193/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Histone tails have a plethora of different post-translational modifications, which are located differently in "open" and "closed" parts of genomes. H3K4me3/H3K79me2 and H4K20me3 are among the histone marks associated with the early establishment of active and inactive chromatin, respectively. One of the most widespread promoter elements is the CCAAT box, bound by the NF-Y trimer. Two of NF-Y subunits have an H2A-H2B-like structure.<h4>Principal findings</h4>We established the causal relationship between NF-Y binding and positioning of methyl marks, by ChIP analysis of mouse and human cells infected with a dominant negative NF-YA: a parallel decrease in NF-Y binding, H3K4me3, H3K79me2 and transcription was observed in promoters that are dependent upon NF-Y. On the contrary, changes in the levels of H3K9-14ac were more subtle. Components of the H3K4 methylating MLL complex are not recruited in the absence of NF-Y. As for repressed promoters, NF-Y removal leads to a decrease in the H4K20me3 mark and deposition of H3K4me3.<h4>Conclusions</h4>Two relevant findings are reported: (i) NF-Y gains access to its genomic locations independently from the presence of methyl histone marks, either positive or negative; (ii) NF-Y binding has profound positive or negative consequences on the deposition of histone methyl marks. Therefore NF-Y is a fundamental switch at the heart of decision between gene activation and repression in CCAAT regulated genes.Giacomo DonatiRaffaella GattaDiletta DolfiniAndrea FossatiMichele CeribelliRoberto MantovaniPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 4, p e2066 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giacomo Donati Raffaella Gatta Diletta Dolfini Andrea Fossati Michele Ceribelli Roberto Mantovani An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
description	<h4>Background</h4>Histone tails have a plethora of different post-translational modifications, which are located differently in "open" and "closed" parts of genomes. H3K4me3/H3K79me2 and H4K20me3 are among the histone marks associated with the early establishment of active and inactive chromatin, respectively. One of the most widespread promoter elements is the CCAAT box, bound by the NF-Y trimer. Two of NF-Y subunits have an H2A-H2B-like structure.<h4>Principal findings</h4>We established the causal relationship between NF-Y binding and positioning of methyl marks, by ChIP analysis of mouse and human cells infected with a dominant negative NF-YA: a parallel decrease in NF-Y binding, H3K4me3, H3K79me2 and transcription was observed in promoters that are dependent upon NF-Y. On the contrary, changes in the levels of H3K9-14ac were more subtle. Components of the H3K4 methylating MLL complex are not recruited in the absence of NF-Y. As for repressed promoters, NF-Y removal leads to a decrease in the H4K20me3 mark and deposition of H3K4me3.<h4>Conclusions</h4>Two relevant findings are reported: (i) NF-Y gains access to its genomic locations independently from the presence of methyl histone marks, either positive or negative; (ii) NF-Y binding has profound positive or negative consequences on the deposition of histone methyl marks. Therefore NF-Y is a fundamental switch at the heart of decision between gene activation and repression in CCAAT regulated genes.
format	article
author	Giacomo Donati Raffaella Gatta Diletta Dolfini Andrea Fossati Michele Ceribelli Roberto Mantovani
author_facet	Giacomo Donati Raffaella Gatta Diletta Dolfini Andrea Fossati Michele Ceribelli Roberto Mantovani
author_sort	Giacomo Donati
title	An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
title_short	An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
title_full	An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
title_fullStr	An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
title_full_unstemmed	An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.
title_sort	nf-y-dependent switch of positive and negative histone methyl marks on ccaat promoters.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/83d09b13b14e4f1688bb4ee883744ab3
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An NF-Y-dependent switch of positive and negative histone methyl marks on CCAAT promoters.

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