Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by their program MHcut.

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Autores principales:	Janin Grajcarek, Jean Monlong, Yoko Nishinaka-Arai, Michiko Nakamura, Miki Nagai, Shiori Matsuo, David Lougheed, Hidetoshi Sakurai, Megumu K. Saito, Guillaume Bourque, Knut Woltjen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/83dd1a923e3149b5847e613384f3d67a
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spelling	oai:doaj.org-article:83dd1a923e3149b5847e613384f3d67a2021-12-02T17:02:17ZGenome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations10.1038/s41467-019-12829-82041-1723https://doaj.org/article/83dd1a923e3149b5847e613384f3d67a2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12829-8https://doaj.org/toc/2041-1723DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by their program MHcut.Janin GrajcarekJean MonlongYoko Nishinaka-AraiMichiko NakamuraMiki NagaiShiori MatsuoDavid LougheedHidetoshi SakuraiMegumu K. SaitoGuillaume BourqueKnut WoltjenNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Janin Grajcarek Jean Monlong Yoko Nishinaka-Arai Michiko Nakamura Miki Nagai Shiori Matsuo David Lougheed Hidetoshi Sakurai Megumu K. Saito Guillaume Bourque Knut Woltjen Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
description	DNA repair by microhomology-mediated end joining creates precise deletions based on flanking microhomologies. Here the authors use CRISPR-Cas9 to recreate pathogenic deletion mutations using existing microhomologies in the human genome identified by their program MHcut.
format	article
author	Janin Grajcarek Jean Monlong Yoko Nishinaka-Arai Michiko Nakamura Miki Nagai Shiori Matsuo David Lougheed Hidetoshi Sakurai Megumu K. Saito Guillaume Bourque Knut Woltjen
author_facet	Janin Grajcarek Jean Monlong Yoko Nishinaka-Arai Michiko Nakamura Miki Nagai Shiori Matsuo David Lougheed Hidetoshi Sakurai Megumu K. Saito Guillaume Bourque Knut Woltjen
author_sort	Janin Grajcarek
title	Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
title_short	Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
title_full	Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
title_fullStr	Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
title_full_unstemmed	Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
title_sort	genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/83dd1a923e3149b5847e613384f3d67a
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Genome-wide microhomologies enable precise template-free editing of biologically relevant deletion mutations

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