GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS

Abstract. Background. Mycobacterium avium subsp. hominissuis (MAH) is an important pathogen responsible for most of the human-associated nontuberculous mycobacteria infections. Over the past few decades the incidence of MAH infections is increasing in Italy, as in many countries worldwide. The prese...

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Autores principales:	M. Menichini, F. Genua, N. Lari, L. Rindi
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2018
Materias:	mycobacterium avium population structure italy vntr loci mycobacteriosis Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/83e64baef9aa42deb49cd9ca405ef2e4
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spelling	oai:doaj.org-article:83e64baef9aa42deb49cd9ca405ef2e42021-11-22T07:09:52ZGENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS2220-76192313-739810.15789/2220-7619-2018-4-441-446https://doaj.org/article/83e64baef9aa42deb49cd9ca405ef2e42018-12-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/825https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Abstract. Background. Mycobacterium avium subsp. hominissuis (MAH) is an important pathogen responsible for most of the human-associated nontuberculous mycobacteria infections. Over the past few decades the incidence of MAH infections is increasing in Italy, as in many countries worldwide. The present study is aimed to elucidate the genetic characteristics of MAH strains isolated from human patients using VNTR typing and to show the genetic relatedness among them. Methods. The genetic diversity of 108 human isolates of MAH was determined by VNTR analysis targeting 8 loci, coded 32, 292, X3, 25, 3, 7, 10 and 47. Results. The VNTR analysis revealed 25 distinct VNTR patterns; of these, 13 patterns were unique, while 12 patterns were shared by 2 or more isolates, thus yielding 12 clusters including a total of 95 isolates. The discriminatory power of our VNTR analysis yielded an HGDI of 0.990, indicating that VNTR typing has an excellent discriminatory power. No association of a particular VNTR pattern with a particular clinical feature, such as the disseminated, pulmonary or extrapulmonary type of infection, was observed. Minimum spanning tree analysis showed that 21 VNTR patterns, occurring either as clustered or unique isolates, differed from the nearest one for one allelic variation. Conclusions. The results obtained through the VNTR analysis showed that most MAH strains displayed a close genetic relationship. This high phylogenetic proximity of the VNTR loci over a long time period supports the concept that the MAH genotype is highly homogeneous in our geographical area, suggesting the hypothesis of the presence of possible sources of infection and transmission pathways at the local level.M. MenichiniF. GenuaN. LariL. RindiSankt-Peterburg : NIIÈM imeni Pasteraarticlemycobacterium aviumpopulation structureitalyvntr locimycobacteriosisInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 8, Iss 4, Pp 441-446 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	mycobacterium avium population structure italy vntr loci mycobacteriosis Infectious and parasitic diseases RC109-216
spellingShingle	mycobacterium avium population structure italy vntr loci mycobacteriosis Infectious and parasitic diseases RC109-216 M. Menichini F. Genua N. Lari L. Rindi GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
description	Abstract. Background. Mycobacterium avium subsp. hominissuis (MAH) is an important pathogen responsible for most of the human-associated nontuberculous mycobacteria infections. Over the past few decades the incidence of MAH infections is increasing in Italy, as in many countries worldwide. The present study is aimed to elucidate the genetic characteristics of MAH strains isolated from human patients using VNTR typing and to show the genetic relatedness among them. Methods. The genetic diversity of 108 human isolates of MAH was determined by VNTR analysis targeting 8 loci, coded 32, 292, X3, 25, 3, 7, 10 and 47. Results. The VNTR analysis revealed 25 distinct VNTR patterns; of these, 13 patterns were unique, while 12 patterns were shared by 2 or more isolates, thus yielding 12 clusters including a total of 95 isolates. The discriminatory power of our VNTR analysis yielded an HGDI of 0.990, indicating that VNTR typing has an excellent discriminatory power. No association of a particular VNTR pattern with a particular clinical feature, such as the disseminated, pulmonary or extrapulmonary type of infection, was observed. Minimum spanning tree analysis showed that 21 VNTR patterns, occurring either as clustered or unique isolates, differed from the nearest one for one allelic variation. Conclusions. The results obtained through the VNTR analysis showed that most MAH strains displayed a close genetic relationship. This high phylogenetic proximity of the VNTR loci over a long time period supports the concept that the MAH genotype is highly homogeneous in our geographical area, suggesting the hypothesis of the presence of possible sources of infection and transmission pathways at the local level.
format	article
author	M. Menichini F. Genua N. Lari L. Rindi
author_facet	M. Menichini F. Genua N. Lari L. Rindi
author_sort	M. Menichini
title	GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
title_short	GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
title_full	GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
title_fullStr	GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
title_full_unstemmed	GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS
title_sort	genetic diversity of mycobacterium avium subsp. hominissuis strains isolated in italy based on vntr loci analysis
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2018
url	https://doaj.org/article/83e64baef9aa42deb49cd9ca405ef2e4
work_keys_str_mv	AT mmenichini geneticdiversityofmycobacteriumaviumsubsphominissuisstrainsisolatedinitalybasedonvntrlocianalysis AT fgenua geneticdiversityofmycobacteriumaviumsubsphominissuisstrainsisolatedinitalybasedonvntrlocianalysis AT nlari geneticdiversityofmycobacteriumaviumsubsphominissuisstrainsisolatedinitalybasedonvntrlocianalysis AT lrindi geneticdiversityofmycobacteriumaviumsubsphominissuisstrainsisolatedinitalybasedonvntrlocianalysis
_version_	1718417927506493440

GENETIC DIVERSITY OF MYCOBACTERIUM AVIUM subsp. HOMINISSUIS STRAINS ISOLATED IN ITALY BASED ON VNTR LOCI ANALYSIS

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