Network organisation and the dynamics of tubules in the endoplasmic reticulum

Abstract The endoplasmic reticulum (ER) is a eukaryotic subcellular organelle composed of tubules and sheet-like areas of membrane connected at junctions. The tubule network is highly dynamic and undergoes rapid and continual rearrangement. There are currently few tools to evaluate network organisat...

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Autores principales:	Hannah T. Perkins, Victoria J. Allan, Thomas A. Waigh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83e6f201f1ce47c7bd622bccb6d53b58
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spelling	oai:doaj.org-article:83e6f201f1ce47c7bd622bccb6d53b582021-12-02T16:28:00ZNetwork organisation and the dynamics of tubules in the endoplasmic reticulum10.1038/s41598-021-94901-22045-2322https://doaj.org/article/83e6f201f1ce47c7bd622bccb6d53b582021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94901-2https://doaj.org/toc/2045-2322Abstract The endoplasmic reticulum (ER) is a eukaryotic subcellular organelle composed of tubules and sheet-like areas of membrane connected at junctions. The tubule network is highly dynamic and undergoes rapid and continual rearrangement. There are currently few tools to evaluate network organisation and dynamics. We quantified ER network organisation in Vero and MRC5 cells, and developed an analysis workflow for dynamics of established tubules in live cells. The persistence length, tubule length, junction coordination number and angles of the network were quantified. Hallmarks of imbalances in ER tension, indications of interactions with microtubules and other subcellular organelles, and active dynamics were observed. Clear differences in dynamic behaviour were observed for established tubules at different positions within the cell using itemset mining. We found that tubules with activity-driven fluctuations were more likely to be located away from the cell periphery and a population of peripheral tubules with no signs of active motion was found.Hannah T. PerkinsVictoria J. AllanThomas A. WaighNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hannah T. Perkins Victoria J. Allan Thomas A. Waigh Network organisation and the dynamics of tubules in the endoplasmic reticulum
description	Abstract The endoplasmic reticulum (ER) is a eukaryotic subcellular organelle composed of tubules and sheet-like areas of membrane connected at junctions. The tubule network is highly dynamic and undergoes rapid and continual rearrangement. There are currently few tools to evaluate network organisation and dynamics. We quantified ER network organisation in Vero and MRC5 cells, and developed an analysis workflow for dynamics of established tubules in live cells. The persistence length, tubule length, junction coordination number and angles of the network were quantified. Hallmarks of imbalances in ER tension, indications of interactions with microtubules and other subcellular organelles, and active dynamics were observed. Clear differences in dynamic behaviour were observed for established tubules at different positions within the cell using itemset mining. We found that tubules with activity-driven fluctuations were more likely to be located away from the cell periphery and a population of peripheral tubules with no signs of active motion was found.
format	article
author	Hannah T. Perkins Victoria J. Allan Thomas A. Waigh
author_facet	Hannah T. Perkins Victoria J. Allan Thomas A. Waigh
author_sort	Hannah T. Perkins
title	Network organisation and the dynamics of tubules in the endoplasmic reticulum
title_short	Network organisation and the dynamics of tubules in the endoplasmic reticulum
title_full	Network organisation and the dynamics of tubules in the endoplasmic reticulum
title_fullStr	Network organisation and the dynamics of tubules in the endoplasmic reticulum
title_full_unstemmed	Network organisation and the dynamics of tubules in the endoplasmic reticulum
title_sort	network organisation and the dynamics of tubules in the endoplasmic reticulum
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/83e6f201f1ce47c7bd622bccb6d53b58
work_keys_str_mv	AT hannahtperkins networkorganisationandthedynamicsoftubulesintheendoplasmicreticulum AT victoriajallan networkorganisationandthedynamicsoftubulesintheendoplasmicreticulum AT thomasawaigh networkorganisationandthedynamicsoftubulesintheendoplasmicreticulum
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Network organisation and the dynamics of tubules in the endoplasmic reticulum

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