Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice

Abstract Very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family that is involved in the uptake of VLDL into cells. Increased hepatic VLDLR under endoplasmic reticulum (ER) stress has been shown to cause fatty liver. In this study, the effect of dietary protein restricti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Yui Oshio, Yuta Hattori, Hatsuho Kamata, Yori Ozaki-Masuzawa, Arisa Seki, Yasutaka Tsuruta, Asako Takenaka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83eab185ef234a689eddb13e6646223a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:83eab185ef234a689eddb13e6646223a
record_format	dspace
spelling	oai:doaj.org-article:83eab185ef234a689eddb13e6646223a2021-12-02T18:03:21ZVery low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice10.1038/s41598-021-87568-22045-2322https://doaj.org/article/83eab185ef234a689eddb13e6646223a2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87568-2https://doaj.org/toc/2045-2322Abstract Very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family that is involved in the uptake of VLDL into cells. Increased hepatic VLDLR under endoplasmic reticulum (ER) stress has been shown to cause fatty liver. In this study, the effect of dietary protein restriction on hepatic VLDLR and the role of VLDLR in fatty liver were investigated using Vldlr knockout (KO) mice. Growing wild-type (WT) and KO mice were fed a control diet containing 20% protein or a low protein diet containing 3% protein for 11 days. In WT mice, the amount of hepatic Vldlr mRNA and VLDLR protein increased by approximately 8- and 7-fold, respectively, due to protein restriction. Vldlr mRNA and protein levels increased in both type 1 and type 2 VLDLR. However, neither Vldlr mRNA nor protein levels were significantly increased in heart, muscle, and adipose tissue, demonstrating that VLDLR increase due to protein restriction occurred in a liver-specific manner. Increased liver triglyceride levels during protein restriction occurred in KO mice to the same extent as in WT mice, indicating that increased VLDLR during protein restriction was not the main cause of fatty liver, which was different from the case of ER stress.Yui OshioYuta HattoriHatsuho KamataYori Ozaki-MasuzawaArisa SekiYasutaka TsurutaAsako TakenakaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yui Oshio Yuta Hattori Hatsuho Kamata Yori Ozaki-Masuzawa Arisa Seki Yasutaka Tsuruta Asako Takenaka Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
description	Abstract Very low-density lipoprotein receptor (VLDLR) is a member of the LDL receptor family that is involved in the uptake of VLDL into cells. Increased hepatic VLDLR under endoplasmic reticulum (ER) stress has been shown to cause fatty liver. In this study, the effect of dietary protein restriction on hepatic VLDLR and the role of VLDLR in fatty liver were investigated using Vldlr knockout (KO) mice. Growing wild-type (WT) and KO mice were fed a control diet containing 20% protein or a low protein diet containing 3% protein for 11 days. In WT mice, the amount of hepatic Vldlr mRNA and VLDLR protein increased by approximately 8- and 7-fold, respectively, due to protein restriction. Vldlr mRNA and protein levels increased in both type 1 and type 2 VLDLR. However, neither Vldlr mRNA nor protein levels were significantly increased in heart, muscle, and adipose tissue, demonstrating that VLDLR increase due to protein restriction occurred in a liver-specific manner. Increased liver triglyceride levels during protein restriction occurred in KO mice to the same extent as in WT mice, indicating that increased VLDLR during protein restriction was not the main cause of fatty liver, which was different from the case of ER stress.
format	article
author	Yui Oshio Yuta Hattori Hatsuho Kamata Yori Ozaki-Masuzawa Arisa Seki Yasutaka Tsuruta Asako Takenaka
author_facet	Yui Oshio Yuta Hattori Hatsuho Kamata Yori Ozaki-Masuzawa Arisa Seki Yasutaka Tsuruta Asako Takenaka
author_sort	Yui Oshio
title	Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
title_short	Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
title_full	Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
title_fullStr	Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
title_full_unstemmed	Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
title_sort	very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/83eab185ef234a689eddb13e6646223a
work_keys_str_mv	AT yuioshio verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT yutahattori verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT hatsuhokamata verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT yoriozakimasuzawa verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT arisaseki verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT yasutakatsuruta verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice AT asakotakenaka verylowdensitylipoproteinreceptorincreasesinaliverspecificmannerduetoproteindeficiencybutdoesnotaffectfattyliverinmice
_version_	1718378763663704064

Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice

Ejemplares similares