Fast objective coupled planar illumination microscopy

Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across camera...

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Auteurs principaux:	Cody J. Greer, Timothy E. Holy
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2019
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca46
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id	oai:doaj.org-article:83ed1483a65b4a03a3e7bebf5960ca46
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spelling	oai:doaj.org-article:83ed1483a65b4a03a3e7bebf5960ca462021-12-02T16:57:52ZFast objective coupled planar illumination microscopy10.1038/s41467-019-12340-02041-1723https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca462019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12340-0https://doaj.org/toc/2041-1723Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across cameras to achieve 40 Hz imaging over thick samples.Cody J. GreerTimothy E. HolyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Cody J. Greer Timothy E. Holy Fast objective coupled planar illumination microscopy
description	Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across cameras to achieve 40 Hz imaging over thick samples.
format	article
author	Cody J. Greer Timothy E. Holy
author_facet	Cody J. Greer Timothy E. Holy
author_sort	Cody J. Greer
title	Fast objective coupled planar illumination microscopy
title_short	Fast objective coupled planar illumination microscopy
title_full	Fast objective coupled planar illumination microscopy
title_fullStr	Fast objective coupled planar illumination microscopy
title_full_unstemmed	Fast objective coupled planar illumination microscopy
title_sort	fast objective coupled planar illumination microscopy
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca46
work_keys_str_mv	AT codyjgreer fastobjectivecoupledplanarilluminationmicroscopy AT timothyeholy fastobjectivecoupledplanarilluminationmicroscopy
_version_	1718382455800463360

Fast objective coupled planar illumination microscopy

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