Fast objective coupled planar illumination microscopy

Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across camera...

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Autores principales: Cody J. Greer, Timothy E. Holy
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca46
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spelling oai:doaj.org-article:83ed1483a65b4a03a3e7bebf5960ca462021-12-02T16:57:52ZFast objective coupled planar illumination microscopy10.1038/s41467-019-12340-02041-1723https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca462019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12340-0https://doaj.org/toc/2041-1723Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across cameras to achieve 40 Hz imaging over thick samples.Cody J. GreerTimothy E. HolyNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Cody J. Greer
Timothy E. Holy
Fast objective coupled planar illumination microscopy
description Light sheet microscopy holds potential for imaging dynamics in 3D biological specimens, but is limited by scan speed and camera acquisition rate. Here the authors address both issues by developing speed-optimized Objective Coupled Planar Illumination and parallelizing image acquisition across cameras to achieve 40 Hz imaging over thick samples.
format article
author Cody J. Greer
Timothy E. Holy
author_facet Cody J. Greer
Timothy E. Holy
author_sort Cody J. Greer
title Fast objective coupled planar illumination microscopy
title_short Fast objective coupled planar illumination microscopy
title_full Fast objective coupled planar illumination microscopy
title_fullStr Fast objective coupled planar illumination microscopy
title_full_unstemmed Fast objective coupled planar illumination microscopy
title_sort fast objective coupled planar illumination microscopy
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/83ed1483a65b4a03a3e7bebf5960ca46
work_keys_str_mv AT codyjgreer fastobjectivecoupledplanarilluminationmicroscopy
AT timothyeholy fastobjectivecoupledplanarilluminationmicroscopy
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