MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.

Since brain tissue is not readily accessible, a new focus in search of biomarkers for schizophrenia is blood-based expression profiling of non-protein coding genes such as microRNAs (miRNAs), which regulate gene expression by inhibiting the translation of messenger RNAs. This study aimed to identify...

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Autores principales:	Chi-Yu Lai, Sung-Liang Yu, Ming H Hsieh, Chun-Houh Chen, Hsuan-Yu Chen, Chun-Chiang Wen, Yung-Hsiang Huang, Po-Chang Hsiao, Chuhsing Kate Hsiao, Chih-Min Liu, Pan-Chyr Yang, Hai-Gwo Hwu, Wei J Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83f40c7e2ec64159acbf173c7d151f3c
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spelling	oai:doaj.org-article:83f40c7e2ec64159acbf173c7d151f3c2021-11-18T06:50:59ZMicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.1932-620310.1371/journal.pone.0021635https://doaj.org/article/83f40c7e2ec64159acbf173c7d151f3c2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21738743/?tool=EBIhttps://doaj.org/toc/1932-6203Since brain tissue is not readily accessible, a new focus in search of biomarkers for schizophrenia is blood-based expression profiling of non-protein coding genes such as microRNAs (miRNAs), which regulate gene expression by inhibiting the translation of messenger RNAs. This study aimed to identify potential miRNA signature for schizophrenia by comparing genome-wide miRNA expression profiles in patients with schizophrenia vs. healthy controls. A genome-wide miRNA expression profiling was performed using a Taqman array of 365 human miRNAs in the mononuclear leukocytes of a learning set of 30 cases and 30 controls. The discriminating performance of potential biomarkers was validated in an independent testing set of 60 cases and 30 controls. The expression levels of the miRNA signature were then evaluated for their correlation with the patients' clinical symptoms, neurocognitive performances, and neurophysiological functions. A seven-miRNA signature (hsa-miR-34a, miR-449a, miR-564, miR-432, miR-548d, miR-572 and miR-652) was derived from a supervised classification with internal cross-validation, with an area under the curve (AUC) of receiver operating characteristics of 93%. The putative signature was then validated in the testing set, with an AUC of 85%. Among these miRNAs, miR-34a was differentially expressed between cases and controls in both the learning (P = 0.005) and the testing set (P = 0.002). These miRNAs were differentially correlated with patients' negative symptoms, neurocognitive performance scores, and event-related potentials. The results indicated that the mononuclear leukocyte-based miRNA profiling is a feasible way to identify biomarkers for schizophrenia, and the seven-miRNA signature warrants further investigation.Chi-Yu LaiSung-Liang YuMing H HsiehChun-Houh ChenHsuan-Yu ChenChun-Chiang WenYung-Hsiang HuangPo-Chang HsiaoChuhsing Kate HsiaoChih-Min LiuPan-Chyr YangHai-Gwo HwuWei J ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e21635 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chi-Yu Lai Sung-Liang Yu Ming H Hsieh Chun-Houh Chen Hsuan-Yu Chen Chun-Chiang Wen Yung-Hsiang Huang Po-Chang Hsiao Chuhsing Kate Hsiao Chih-Min Liu Pan-Chyr Yang Hai-Gwo Hwu Wei J Chen MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
description	Since brain tissue is not readily accessible, a new focus in search of biomarkers for schizophrenia is blood-based expression profiling of non-protein coding genes such as microRNAs (miRNAs), which regulate gene expression by inhibiting the translation of messenger RNAs. This study aimed to identify potential miRNA signature for schizophrenia by comparing genome-wide miRNA expression profiles in patients with schizophrenia vs. healthy controls. A genome-wide miRNA expression profiling was performed using a Taqman array of 365 human miRNAs in the mononuclear leukocytes of a learning set of 30 cases and 30 controls. The discriminating performance of potential biomarkers was validated in an independent testing set of 60 cases and 30 controls. The expression levels of the miRNA signature were then evaluated for their correlation with the patients' clinical symptoms, neurocognitive performances, and neurophysiological functions. A seven-miRNA signature (hsa-miR-34a, miR-449a, miR-564, miR-432, miR-548d, miR-572 and miR-652) was derived from a supervised classification with internal cross-validation, with an area under the curve (AUC) of receiver operating characteristics of 93%. The putative signature was then validated in the testing set, with an AUC of 85%. Among these miRNAs, miR-34a was differentially expressed between cases and controls in both the learning (P = 0.005) and the testing set (P = 0.002). These miRNAs were differentially correlated with patients' negative symptoms, neurocognitive performance scores, and event-related potentials. The results indicated that the mononuclear leukocyte-based miRNA profiling is a feasible way to identify biomarkers for schizophrenia, and the seven-miRNA signature warrants further investigation.
format	article
author	Chi-Yu Lai Sung-Liang Yu Ming H Hsieh Chun-Houh Chen Hsuan-Yu Chen Chun-Chiang Wen Yung-Hsiang Huang Po-Chang Hsiao Chuhsing Kate Hsiao Chih-Min Liu Pan-Chyr Yang Hai-Gwo Hwu Wei J Chen
author_facet	Chi-Yu Lai Sung-Liang Yu Ming H Hsieh Chun-Houh Chen Hsuan-Yu Chen Chun-Chiang Wen Yung-Hsiang Huang Po-Chang Hsiao Chuhsing Kate Hsiao Chih-Min Liu Pan-Chyr Yang Hai-Gwo Hwu Wei J Chen
author_sort	Chi-Yu Lai
title	MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
title_short	MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
title_full	MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
title_fullStr	MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
title_full_unstemmed	MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.
title_sort	microrna expression aberration as potential peripheral blood biomarkers for schizophrenia.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/83f40c7e2ec64159acbf173c7d151f3c
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MicroRNA expression aberration as potential peripheral blood biomarkers for schizophrenia.

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