TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells

Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double strande...

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Autores principales:	Sumitra Shankar, Deepti Prasad, Rahul Sanawar, Ani V. Das, M. Radhakrishna Pillai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83f53160399a4bffbbcca8110f2ae7a9
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spelling	oai:doaj.org-article:83f53160399a4bffbbcca8110f2ae7a92021-12-02T16:08:12ZTALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells10.1038/s41598-017-05696-02045-2322https://doaj.org/article/83f53160399a4bffbbcca8110f2ae7a92017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05696-0https://doaj.org/toc/2045-2322Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double stranded breaks at specific sites in the genome. In here, we have designed specific TALENs to target E7 and analyzed their efficiency in inducing cell death in cervical cancer cells. We found that designed TALENs could yield about 10–12% editing activity as observed from T7E1 and nuclease resistance assays. Down-regulation of E7 and E6 was further evident at the transcript as well as proteins levels indicating that the selected TALENs were effective. TALEN-mediated E7 editing led to cell death as ascertained by cell cycle and Annexin V assays. Annexin profiling suggested that cell death could be due to necrosis as observed by upregulation of necrotic markers such as LDH A, Rip-1, and Cyclophilin A. Necrosis appears to be a better therapeutic response as it could further activate pro-inflammatory cytokines to attract immune cells to eliminate HPV-integrated cells and therefore TALEN editing strategy has the potential to be a promising tool as an adjuvant therapy in cervical cancer along with surgery.Sumitra ShankarDeepti PrasadRahul SanawarAni V. DasM. Radhakrishna PillaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sumitra Shankar Deepti Prasad Rahul Sanawar Ani V. Das M. Radhakrishna Pillai TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
description	Abstract Human Papillomavirus E7 and E6 oncoproteins have been considered as suitable candidate anti-viral targets since they cause malignant conversion in cervical cancers. Transcription Activator-Like Effector Nucleases (TALENs) are recent editing tools to knockout genes by inducing double stranded breaks at specific sites in the genome. In here, we have designed specific TALENs to target E7 and analyzed their efficiency in inducing cell death in cervical cancer cells. We found that designed TALENs could yield about 10–12% editing activity as observed from T7E1 and nuclease resistance assays. Down-regulation of E7 and E6 was further evident at the transcript as well as proteins levels indicating that the selected TALENs were effective. TALEN-mediated E7 editing led to cell death as ascertained by cell cycle and Annexin V assays. Annexin profiling suggested that cell death could be due to necrosis as observed by upregulation of necrotic markers such as LDH A, Rip-1, and Cyclophilin A. Necrosis appears to be a better therapeutic response as it could further activate pro-inflammatory cytokines to attract immune cells to eliminate HPV-integrated cells and therefore TALEN editing strategy has the potential to be a promising tool as an adjuvant therapy in cervical cancer along with surgery.
format	article
author	Sumitra Shankar Deepti Prasad Rahul Sanawar Ani V. Das M. Radhakrishna Pillai
author_facet	Sumitra Shankar Deepti Prasad Rahul Sanawar Ani V. Das M. Radhakrishna Pillai
author_sort	Sumitra Shankar
title	TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
title_short	TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
title_full	TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
title_fullStr	TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
title_full_unstemmed	TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells
title_sort	talen based hpv-e7 editing triggers necrotic cell death in cervical cancer cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/83f53160399a4bffbbcca8110f2ae7a9
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TALEN based HPV-E7 editing triggers necrotic cell death in cervical cancer cells

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