A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis

Rate-limiting steps in synthetic metabolic pathways are difficult to identify. Here, the authors monitor metabolite dynamics and apply kinetic modelling during the start-up phase of the Bacillus subtilisGlcNAc pathway to discover a futile cycle, allowing them to identify a more productive strain.

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Autores principales:	Yanfeng Liu, Hannes Link, Long Liu, Guocheng Du, Jian Chen, Uwe Sauer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/83f9a7d8728f4a97b3cde7d8b6fe982a
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spelling	oai:doaj.org-article:83f9a7d8728f4a97b3cde7d8b6fe982a2021-12-02T14:39:31ZA dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis10.1038/ncomms119332041-1723https://doaj.org/article/83f9a7d8728f4a97b3cde7d8b6fe982a2016-06-01T00:00:00Zhttps://doi.org/10.1038/ncomms11933https://doaj.org/toc/2041-1723Rate-limiting steps in synthetic metabolic pathways are difficult to identify. Here, the authors monitor metabolite dynamics and apply kinetic modelling during the start-up phase of the Bacillus subtilisGlcNAc pathway to discover a futile cycle, allowing them to identify a more productive strain.Yanfeng LiuHannes LinkLong LiuGuocheng DuJian ChenUwe SauerNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yanfeng Liu Hannes Link Long Liu Guocheng Du Jian Chen Uwe Sauer A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
description	Rate-limiting steps in synthetic metabolic pathways are difficult to identify. Here, the authors monitor metabolite dynamics and apply kinetic modelling during the start-up phase of the Bacillus subtilisGlcNAc pathway to discover a futile cycle, allowing them to identify a more productive strain.
format	article
author	Yanfeng Liu Hannes Link Long Liu Guocheng Du Jian Chen Uwe Sauer
author_facet	Yanfeng Liu Hannes Link Long Liu Guocheng Du Jian Chen Uwe Sauer
author_sort	Yanfeng Liu
title	A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
title_short	A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
title_full	A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
title_fullStr	A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
title_full_unstemmed	A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis
title_sort	dynamic pathway analysis approach reveals a limiting futile cycle in n-acetylglucosamine overproducing bacillus subtilis
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/83f9a7d8728f4a97b3cde7d8b6fe982a
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A dynamic pathway analysis approach reveals a limiting futile cycle in N-acetylglucosamine overproducing Bacillus subtilis

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