Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease

Maize lethal necrosis (MLN) is a viral disease with a devastating effect on maize production. Developing and deploying improved varieties with resistance to the disease is important to effectively control MLN; however, little is known about the causal genes and molecular mechanism(s) underlying MLN...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ann Murithi, Michael S. Olsen, Daniel B. Kwemoi, Ogugo Veronica, Berhanu Tadesse Ertiro, Suresh L. M., Yoseph Beyene, Biswanath Das, Boddupalli M. Prasanna, Manje Gowda
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	maize lethal necrosis genome-wide association study F2 population selective genotyping disease resistance Genetics QH426-470
Acceso en línea:	https://doaj.org/article/83fa622d20954baa87ea2118b936c0d8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:83fa622d20954baa87ea2118b936c0d8
record_format	dspace
spelling	oai:doaj.org-article:83fa622d20954baa87ea2118b936c0d82021-11-19T06:09:05ZDiscovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease1664-802110.3389/fgene.2021.767883https://doaj.org/article/83fa622d20954baa87ea2118b936c0d82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.767883/fullhttps://doaj.org/toc/1664-8021Maize lethal necrosis (MLN) is a viral disease with a devastating effect on maize production. Developing and deploying improved varieties with resistance to the disease is important to effectively control MLN; however, little is known about the causal genes and molecular mechanism(s) underlying MLN resistance. Screening thousands of maize inbred lines revealed KS23-5 and KS23-6 as two of the most promising donors of MLN resistance alleles. KS23-5 and KS23-6 lines were earlier developed at the University of Hawaii, United States, on the basis of a source population constituted using germplasm from Kasetsart University, Thailand. Both linkage mapping and association mapping approaches were used to discover and validate genomic regions associated with MLN resistance. Selective genotyping of resistant and susceptible individuals within large F2 populations coupled with genome-wide association study identified a major-effect QTL (qMLN06_157) on chromosome 6 for MLN disease severity score and area under the disease progress curve values in all three F2 populations involving one of the KS23 lines as a parent. The major-effect QTL (qMLN06_157) is recessively inherited and explained 55%–70% of the phenotypic variation with an approximately 6 Mb confidence interval. Linkage mapping in three F3 populations and three F2 populations involving KS23-5 or KS23-6 as one of the parents confirmed the presence of this major-effect QTL on chromosome 6, demonstrating the efficacy of the KS23 allele at qMLN06.157 in varying populations. This QTL could not be identified in population that was not derived using KS23 lines. Validation of this QTL in six F2 populations with 20 SNPs closely linked with qMLN06.157 was further confirmed its consistent expression across populations and its recessive nature of inheritance. On the basis of the consistent and effective resistance afforded by the KS23 allele at qMLN06.157, the QTL can be used in both marker-assisted forward breeding and marker-assisted backcrossing schemes to improve MLN resistance of breeding populations and key lines for eastern Africa.Ann MurithiAnn MurithiMichael S. OlsenDaniel B. KwemoiOgugo VeronicaBerhanu Tadesse ErtiroSuresh L. M.Yoseph BeyeneBiswanath DasBoddupalli M. PrasannaManje GowdaFrontiers Media S.A.articlemaize lethal necrosisgenome-wide association studyF2 populationselective genotypingdisease resistanceGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	maize lethal necrosis genome-wide association study F2 population selective genotyping disease resistance Genetics QH426-470
spellingShingle	maize lethal necrosis genome-wide association study F2 population selective genotyping disease resistance Genetics QH426-470 Ann Murithi Ann Murithi Michael S. Olsen Daniel B. Kwemoi Ogugo Veronica Berhanu Tadesse Ertiro Suresh L. M. Yoseph Beyene Biswanath Das Boddupalli M. Prasanna Manje Gowda Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
description	Maize lethal necrosis (MLN) is a viral disease with a devastating effect on maize production. Developing and deploying improved varieties with resistance to the disease is important to effectively control MLN; however, little is known about the causal genes and molecular mechanism(s) underlying MLN resistance. Screening thousands of maize inbred lines revealed KS23-5 and KS23-6 as two of the most promising donors of MLN resistance alleles. KS23-5 and KS23-6 lines were earlier developed at the University of Hawaii, United States, on the basis of a source population constituted using germplasm from Kasetsart University, Thailand. Both linkage mapping and association mapping approaches were used to discover and validate genomic regions associated with MLN resistance. Selective genotyping of resistant and susceptible individuals within large F2 populations coupled with genome-wide association study identified a major-effect QTL (qMLN06_157) on chromosome 6 for MLN disease severity score and area under the disease progress curve values in all three F2 populations involving one of the KS23 lines as a parent. The major-effect QTL (qMLN06_157) is recessively inherited and explained 55%–70% of the phenotypic variation with an approximately 6 Mb confidence interval. Linkage mapping in three F3 populations and three F2 populations involving KS23-5 or KS23-6 as one of the parents confirmed the presence of this major-effect QTL on chromosome 6, demonstrating the efficacy of the KS23 allele at qMLN06.157 in varying populations. This QTL could not be identified in population that was not derived using KS23 lines. Validation of this QTL in six F2 populations with 20 SNPs closely linked with qMLN06.157 was further confirmed its consistent expression across populations and its recessive nature of inheritance. On the basis of the consistent and effective resistance afforded by the KS23 allele at qMLN06.157, the QTL can be used in both marker-assisted forward breeding and marker-assisted backcrossing schemes to improve MLN resistance of breeding populations and key lines for eastern Africa.
format	article
author	Ann Murithi Ann Murithi Michael S. Olsen Daniel B. Kwemoi Ogugo Veronica Berhanu Tadesse Ertiro Suresh L. M. Yoseph Beyene Biswanath Das Boddupalli M. Prasanna Manje Gowda
author_facet	Ann Murithi Ann Murithi Michael S. Olsen Daniel B. Kwemoi Ogugo Veronica Berhanu Tadesse Ertiro Suresh L. M. Yoseph Beyene Biswanath Das Boddupalli M. Prasanna Manje Gowda
author_sort	Ann Murithi
title	Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
title_short	Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
title_full	Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
title_fullStr	Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
title_full_unstemmed	Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease
title_sort	discovery and validation of a recessively inherited major-effect qtl conferring resistance to maize lethal necrosis (mln) disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/83fa622d20954baa87ea2118b936c0d8
work_keys_str_mv	AT annmurithi discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT annmurithi discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT michaelsolsen discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT danielbkwemoi discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT ogugoveronica discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT berhanutadesseertiro discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT sureshlm discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT yosephbeyene discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT biswanathdas discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT boddupallimprasanna discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease AT manjegowda discoveryandvalidationofarecessivelyinheritedmajoreffectqtlconferringresistancetomaizelethalnecrosismlndisease
_version_	1718420323917889536

Discovery and Validation of a Recessively Inherited Major-Effect QTL Conferring Resistance to Maize Lethal Necrosis (MLN) Disease

Ejemplares similares