Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.

Methanococcus maripaludis is an archaeon with two studied surface appendages, archaella and type IV-like pili. Previously, the major structural pilin was identified as MMP1685 and three additional proteins were designated as minor pilins (EpdA, EpdB and EpdC). All of the proteins are likely processe...

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Autores principales:	Divya B Nair, Daniel K C Chung, James Schneider, Kaoru Uchida, Shin-Ichi Aizawa, Ken F Jarrell
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/83ff7e32f8e44163a5b8753626620bc1
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spelling	oai:doaj.org-article:83ff7e32f8e44163a5b8753626620bc12021-11-18T08:39:52ZIdentification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.1932-620310.1371/journal.pone.0083961https://doaj.org/article/83ff7e32f8e44163a5b8753626620bc12013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24386316/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Methanococcus maripaludis is an archaeon with two studied surface appendages, archaella and type IV-like pili. Previously, the major structural pilin was identified as MMP1685 and three additional proteins were designated as minor pilins (EpdA, EpdB and EpdC). All of the proteins are likely processed by the pilin-specific prepilin peptidase EppA. Six other genes were identified earlier as likely encoding pilin proteins processed also by EppA. In this study, each of the six genes (mmp0528, mmp0600, mmp0601, mmp0709, mmp0903 and mmp1283) was deleted and the mutants examined by electron microscopy to determine their essentiality for pili formation. While mRNA transcripts of all genes were detected by RT-PCR, only the deletion of mmp1283 led to nonpiliated cells. This strain could be complemented back to a piliated state by supplying a wildtype copy of the mmp1283 gene in trans. This study adds to the complexity of the type IV pili system in M. maripaludis and raises questions about the functions of the remaining five pilin-like genes and whether M. maripaludis under other growth conditions may be able to assemble additional pili-like structures.Divya B NairDaniel K C ChungJames SchneiderKaoru UchidaShin-Ichi AizawaKen F JarrellPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e83961 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Divya B Nair Daniel K C Chung James Schneider Kaoru Uchida Shin-Ichi Aizawa Ken F Jarrell Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
description	Methanococcus maripaludis is an archaeon with two studied surface appendages, archaella and type IV-like pili. Previously, the major structural pilin was identified as MMP1685 and three additional proteins were designated as minor pilins (EpdA, EpdB and EpdC). All of the proteins are likely processed by the pilin-specific prepilin peptidase EppA. Six other genes were identified earlier as likely encoding pilin proteins processed also by EppA. In this study, each of the six genes (mmp0528, mmp0600, mmp0601, mmp0709, mmp0903 and mmp1283) was deleted and the mutants examined by electron microscopy to determine their essentiality for pili formation. While mRNA transcripts of all genes were detected by RT-PCR, only the deletion of mmp1283 led to nonpiliated cells. This strain could be complemented back to a piliated state by supplying a wildtype copy of the mmp1283 gene in trans. This study adds to the complexity of the type IV pili system in M. maripaludis and raises questions about the functions of the remaining five pilin-like genes and whether M. maripaludis under other growth conditions may be able to assemble additional pili-like structures.
format	article
author	Divya B Nair Daniel K C Chung James Schneider Kaoru Uchida Shin-Ichi Aizawa Ken F Jarrell
author_facet	Divya B Nair Daniel K C Chung James Schneider Kaoru Uchida Shin-Ichi Aizawa Ken F Jarrell
author_sort	Divya B Nair
title	Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
title_short	Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
title_full	Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
title_fullStr	Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
title_full_unstemmed	Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.
title_sort	identification of an additional minor pilin essential for piliation in the archaeon methanococcus maripaludis.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/83ff7e32f8e44163a5b8753626620bc1
work_keys_str_mv	AT divyabnair identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis AT danielkcchung identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis AT jamesschneider identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis AT kaoruuchida identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis AT shinichiaizawa identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis AT kenfjarrell identificationofanadditionalminorpilinessentialforpiliationinthearchaeonmethanococcusmaripaludis
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Identification of an additional minor pilin essential for piliation in the archaeon Methanococcus maripaludis.

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