Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles

ABSTRACT Inorganic pyrophosphate (PPi) is a by-product of biosynthetic reactions and has bioenergetic and regulatory roles in a variety of cells. Here we show that PPi and other pyrophosphate-containing compounds, including polyphosphate (polyP), can stimulate sodium-dependent depolarization of the...

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Autores principales:	Evgeniy Potapenko, Ciro D. Cordeiro, Guozhong Huang, Roberto Docampo
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	SPX domain Saccharomyces cerevisiae Trypanosoma brucei Xenopus laevis acidocalcisome phosphate-sodium symporter Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/8406d6ebc9084f0c9049b7819072ecab
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spelling	oai:doaj.org-article:8406d6ebc9084f0c9049b7819072ecab2021-11-15T15:22:21ZPyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles10.1128/mSphere.00045-192379-5042https://doaj.org/article/8406d6ebc9084f0c9049b7819072ecab2019-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00045-19https://doaj.org/toc/2379-5042ABSTRACT Inorganic pyrophosphate (PPi) is a by-product of biosynthetic reactions and has bioenergetic and regulatory roles in a variety of cells. Here we show that PPi and other pyrophosphate-containing compounds, including polyphosphate (polyP), can stimulate sodium-dependent depolarization of the membrane potential and Pi conductance in Xenopus oocytes expressing a Saccharomyces cerevisiae or Trypanosoma brucei Na+/Pi symporter. PPi is not taken up by Xenopus oocytes, and deletion of the TbPho91 SPX domain abolished its depolarizing effect. PPi generated outward currents in Na+/Pi-loaded giant vacuoles prepared from wild-type or pho91Δ yeast strains expressing TbPHO91 but not from the pho91Δ strains. Our results suggest that PPi, at physiological concentrations, can function as a signaling molecule releasing Pi from S. cerevisiae vacuoles and T. brucei acidocalcisomes. IMPORTANCE Acidocalcisomes, first described in trypanosomes and known to be present in a variety of cells, have similarities with S. cerevisiae vacuoles in their structure and composition. Both organelles share a Na+/Pi symporter involved in Pi release to the cytosol, where it is needed for biosynthetic reactions. Here we show that PPi, at physiological cytosolic concentrations, stimulates the symporter expressed in either Xenopus oocytes or yeast vacuoles via its SPX domain, revealing a signaling role of this molecule.Evgeniy PotapenkoCiro D. CordeiroGuozhong HuangRoberto DocampoAmerican Society for MicrobiologyarticleSPX domainSaccharomyces cerevisiaeTrypanosoma bruceiXenopus laevisacidocalcisomephosphate-sodium symporterMicrobiologyQR1-502ENmSphere, Vol 4, Iss 2 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	SPX domain Saccharomyces cerevisiae Trypanosoma brucei Xenopus laevis acidocalcisome phosphate-sodium symporter Microbiology QR1-502
spellingShingle	SPX domain Saccharomyces cerevisiae Trypanosoma brucei Xenopus laevis acidocalcisome phosphate-sodium symporter Microbiology QR1-502 Evgeniy Potapenko Ciro D. Cordeiro Guozhong Huang Roberto Docampo Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
description	ABSTRACT Inorganic pyrophosphate (PPi) is a by-product of biosynthetic reactions and has bioenergetic and regulatory roles in a variety of cells. Here we show that PPi and other pyrophosphate-containing compounds, including polyphosphate (polyP), can stimulate sodium-dependent depolarization of the membrane potential and Pi conductance in Xenopus oocytes expressing a Saccharomyces cerevisiae or Trypanosoma brucei Na+/Pi symporter. PPi is not taken up by Xenopus oocytes, and deletion of the TbPho91 SPX domain abolished its depolarizing effect. PPi generated outward currents in Na+/Pi-loaded giant vacuoles prepared from wild-type or pho91Δ yeast strains expressing TbPHO91 but not from the pho91Δ strains. Our results suggest that PPi, at physiological concentrations, can function as a signaling molecule releasing Pi from S. cerevisiae vacuoles and T. brucei acidocalcisomes. IMPORTANCE Acidocalcisomes, first described in trypanosomes and known to be present in a variety of cells, have similarities with S. cerevisiae vacuoles in their structure and composition. Both organelles share a Na+/Pi symporter involved in Pi release to the cytosol, where it is needed for biosynthetic reactions. Here we show that PPi, at physiological cytosolic concentrations, stimulates the symporter expressed in either Xenopus oocytes or yeast vacuoles via its SPX domain, revealing a signaling role of this molecule.
format	article
author	Evgeniy Potapenko Ciro D. Cordeiro Guozhong Huang Roberto Docampo
author_facet	Evgeniy Potapenko Ciro D. Cordeiro Guozhong Huang Roberto Docampo
author_sort	Evgeniy Potapenko
title	Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
title_short	Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
title_full	Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
title_fullStr	Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
title_full_unstemmed	Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles
title_sort	pyrophosphate stimulates the phosphate-sodium symporter of <italic toggle="yes">trypanosoma brucei</italic> acidocalcisomes and <italic toggle="yes">saccharomyces cerevisiae</italic> vacuoles
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/8406d6ebc9084f0c9049b7819072ecab
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Pyrophosphate Stimulates the Phosphate-Sodium Symporter of <italic toggle="yes">Trypanosoma brucei</italic> Acidocalcisomes and <italic toggle="yes">Saccharomyces cerevisiae</italic> Vacuoles

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