Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

Abstract Identifying distinct normal extracellular matrix (ECM) features from pathology is of the upmost clinical importance for laryngeal diagnostics and therapy. Despite remarkable histological contributions, our understanding of the vocal fold (VF) physiology remains murky. The emerging field of...

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Autores principales:	Fouzi Benboujja, Chri stopher Hartnick
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8409f09702234f14aefbeb42f178751c
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spelling	oai:doaj.org-article:8409f09702234f14aefbeb42f178751c2021-12-02T13:24:07ZQuantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography10.1038/s41598-021-82157-92045-2322https://doaj.org/article/8409f09702234f14aefbeb42f178751c2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82157-9https://doaj.org/toc/2045-2322Abstract Identifying distinct normal extracellular matrix (ECM) features from pathology is of the upmost clinical importance for laryngeal diagnostics and therapy. Despite remarkable histological contributions, our understanding of the vocal fold (VF) physiology remains murky. The emerging field of non-invasive 3D optical imaging may be well-suited to unravel the complexity of the VF microanatomy. This study focused on characterizing the entire VF ECM in length and depth with optical imaging. A quantitative morphometric evaluation of the human vocal fold lamina propria using two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and optical coherence tomography (OCT) was investigated. Fibrillar morphological features, such as fiber diameter, orientation, anisotropy, waviness and second-order statistics features were evaluated and compared according to their spatial distribution. The evidence acquired in this study suggests that the VF ECM is not a strict discrete three-layer structure as traditionally described but instead a continuous assembly of different fibrillar arrangement anchored by predominant collagen transitions zones. We demonstrated that the ECM composition is distinct and markedly thinned in the anterior one-third of itself, which may play a role in the development of some laryngeal diseases. We further examined and extracted the relationship between OCT and multiphoton imaging, promoting correspondences that could lead to accurate 3D mapping of the VF architecture in real-time during phonosurgeries. As miniaturization of optical probes is consistently improving, a clinical translation of OCT imaging and multiphoton imaging, with valuable qualitative and quantitative features, may have significant implications for treating voice disorders.Fouzi BenboujjaChri stopher HartnickNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fouzi Benboujja Chri stopher Hartnick Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
description	Abstract Identifying distinct normal extracellular matrix (ECM) features from pathology is of the upmost clinical importance for laryngeal diagnostics and therapy. Despite remarkable histological contributions, our understanding of the vocal fold (VF) physiology remains murky. The emerging field of non-invasive 3D optical imaging may be well-suited to unravel the complexity of the VF microanatomy. This study focused on characterizing the entire VF ECM in length and depth with optical imaging. A quantitative morphometric evaluation of the human vocal fold lamina propria using two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and optical coherence tomography (OCT) was investigated. Fibrillar morphological features, such as fiber diameter, orientation, anisotropy, waviness and second-order statistics features were evaluated and compared according to their spatial distribution. The evidence acquired in this study suggests that the VF ECM is not a strict discrete three-layer structure as traditionally described but instead a continuous assembly of different fibrillar arrangement anchored by predominant collagen transitions zones. We demonstrated that the ECM composition is distinct and markedly thinned in the anterior one-third of itself, which may play a role in the development of some laryngeal diseases. We further examined and extracted the relationship between OCT and multiphoton imaging, promoting correspondences that could lead to accurate 3D mapping of the VF architecture in real-time during phonosurgeries. As miniaturization of optical probes is consistently improving, a clinical translation of OCT imaging and multiphoton imaging, with valuable qualitative and quantitative features, may have significant implications for treating voice disorders.
format	article
author	Fouzi Benboujja Chri stopher Hartnick
author_facet	Fouzi Benboujja Chri stopher Hartnick
author_sort	Fouzi Benboujja
title	Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
title_short	Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
title_full	Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
title_fullStr	Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
title_full_unstemmed	Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
title_sort	quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/8409f09702234f14aefbeb42f178751c
work_keys_str_mv	AT fouzibenboujja quantitativeevaluationofthehumanvocalfoldextracellularmatrixusingmultiphotonmicroscopyandopticalcoherencetomography AT christopherhartnick quantitativeevaluationofthehumanvocalfoldextracellularmatrixusingmultiphotonmicroscopyandopticalcoherencetomography
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Quantitative evaluation of the human vocal fold extracellular matrix using multiphoton microscopy and optical coherence tomography

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