Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.

The effects of the herbicide Paraquat were investigated in honey bee larvae with attention focused on oenocytes. Honey bee larvae were exposed to Paraquat at different concentrations in the food: 0, 0.001, 0.01, 0.1 and 1 µg/kg. In controls, between 24 h and 48 h, oenocytes grew from 630.1 to 1643.8...

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Autores principales:	Marianne Cousin, Elaine Silva-Zacarin, André Kretzschmar, Mohamed El Maataoui, Jean-Luc Brunet, Luc P Belzunces
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8424532231254c4da665891d99aea533
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spelling	oai:doaj.org-article:8424532231254c4da665891d99aea5332021-11-18T07:43:59ZSize changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.1932-620310.1371/journal.pone.0065693https://doaj.org/article/8424532231254c4da665891d99aea5332013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23724149/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The effects of the herbicide Paraquat were investigated in honey bee larvae with attention focused on oenocytes. Honey bee larvae were exposed to Paraquat at different concentrations in the food: 0, 0.001, 0.01, 0.1 and 1 µg/kg. In controls, between 24 h and 48 h, oenocytes grew from 630.1 to 1643.8 µm(2) while nuclei changed in size from 124.9 to 245.6 µm(2). At 24 h, Paraquat induced a slight decrease in the size of oenocytes and nuclei. N-acetylcysteine (NAC), an antioxidant substance, slightly lowered the effects of Paraquat. At 48 h, Paraquat elicited a strong concentration-dependent decrease in the size of oenocytes, even at the lowest concentration. NAC reversed the effect of Paraquat at a concentration of ≥0.01 µg/kg. This reversion suggested different modes of action of Paraquat, with an oxidant action prevalent at concentrations ≥0.01 µg/kg. This study is the first which reports an effect of a pesticide at the very low concentration of 1 ng/kg, a concentration below the detection limits of the most efficient analytic methods. It shows that chemicals, including pesticides, are likely to have a potential impact at such exposure levels. We also suggest that Paraquat could be used as a suitable tool for investigating the functions of oenocytes.Marianne CousinElaine Silva-ZacarinAndré KretzschmarMohamed El MaataouiJean-Luc BrunetLuc P BelzuncesPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e65693 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marianne Cousin Elaine Silva-Zacarin André Kretzschmar Mohamed El Maataoui Jean-Luc Brunet Luc P Belzunces Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
description	The effects of the herbicide Paraquat were investigated in honey bee larvae with attention focused on oenocytes. Honey bee larvae were exposed to Paraquat at different concentrations in the food: 0, 0.001, 0.01, 0.1 and 1 µg/kg. In controls, between 24 h and 48 h, oenocytes grew from 630.1 to 1643.8 µm(2) while nuclei changed in size from 124.9 to 245.6 µm(2). At 24 h, Paraquat induced a slight decrease in the size of oenocytes and nuclei. N-acetylcysteine (NAC), an antioxidant substance, slightly lowered the effects of Paraquat. At 48 h, Paraquat elicited a strong concentration-dependent decrease in the size of oenocytes, even at the lowest concentration. NAC reversed the effect of Paraquat at a concentration of ≥0.01 µg/kg. This reversion suggested different modes of action of Paraquat, with an oxidant action prevalent at concentrations ≥0.01 µg/kg. This study is the first which reports an effect of a pesticide at the very low concentration of 1 ng/kg, a concentration below the detection limits of the most efficient analytic methods. It shows that chemicals, including pesticides, are likely to have a potential impact at such exposure levels. We also suggest that Paraquat could be used as a suitable tool for investigating the functions of oenocytes.
format	article
author	Marianne Cousin Elaine Silva-Zacarin André Kretzschmar Mohamed El Maataoui Jean-Luc Brunet Luc P Belzunces
author_facet	Marianne Cousin Elaine Silva-Zacarin André Kretzschmar Mohamed El Maataoui Jean-Luc Brunet Luc P Belzunces
author_sort	Marianne Cousin
title	Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
title_short	Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
title_full	Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
title_fullStr	Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
title_full_unstemmed	Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.
title_sort	size changes in honey bee larvae oenocytes induced by exposure to paraquat at very low concentrations.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/8424532231254c4da665891d99aea533
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Size changes in honey bee larvae oenocytes induced by exposure to Paraquat at very low concentrations.

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