Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: Lavandula viridis L’Hér and Thymus lotocephalus G. López and R. Morales

Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe3O...

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Autores principales:	Sandra Gonçalves, Inês Mansinhos, Raquel Rodríguez-Solana, Gema Pereira-Caro, José Manuel Moreno-Rojas, Anabela Romano
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant activity enzymes inhibition in vitro culture nanoparticles phenolics rosmarinic acid Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/844147f39019464380f866b1dd728110
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spelling	oai:doaj.org-article:844147f39019464380f866b1dd7281102021-11-11T18:26:41ZImpact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales10.3390/molecules262164271420-3049https://doaj.org/article/844147f39019464380f866b1dd7281102021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6427https://doaj.org/toc/1420-3049Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe<sub>3</sub>O<sub>4</sub>) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical parameters of in vitro shoot cultures of <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales (Lamiaceae), as well as on phenolic profile and bioactivity (antioxidant and enzyme inhibition capacities). Although some decreases in shoot number and length were observed in response to NPs, biomass production was not affected or was improved in both species. Most NPs treatments decreased total chlorophyll and carotenoid contents and increased malondialdehyde levels, an indicator of lipid peroxidation, in both species. HPLC-HR-MS analysis led to the identification of thirteen and twelve phenolic compounds, respectively, in <i>L. viridis</i> and <i>T. lotocephalus</i> extracts, being rosmarinic acid the major compound found in all the extracts. ZnO and Fe<sub>3</sub>O<sub>4</sub> NPs induced an increase in total phenolic and rosmarinic acid contents in <i>T. lotocephalus</i> extracts. Additionally, some NPs treatments also increased antioxidant activity in extracts from this species and the opposite was observed for <i>L. viridis</i>. The capacity of the extracts to inhibit tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes was not considerably affected. Overall, NPs had a significant impact on different parameters of <i>L. viridis</i> and <i>T. lotocephalus</i> in vitro shoot cultures, although the results varied with the species and NPs type.Sandra GonçalvesInês MansinhosRaquel Rodríguez-SolanaGema Pereira-CaroJosé Manuel Moreno-RojasAnabela RomanoMDPI AGarticleantioxidant activityenzymes inhibitionin vitro culturenanoparticlesphenolicsrosmarinic acidOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6427, p 6427 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant activity enzymes inhibition in vitro culture nanoparticles phenolics rosmarinic acid Organic chemistry QD241-441
spellingShingle	antioxidant activity enzymes inhibition in vitro culture nanoparticles phenolics rosmarinic acid Organic chemistry QD241-441 Sandra Gonçalves Inês Mansinhos Raquel Rodríguez-Solana Gema Pereira-Caro José Manuel Moreno-Rojas Anabela Romano Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
description	Nanoparticles (NPs) recently emerged as new chemical elicitors acting as signaling agents affecting several processes in plant metabolism. The aim of this work was to investigate the impact of the addition of copper oxide (CuO), zinc oxide (ZnO) and iron oxide (Fe<sub>3</sub>O<sub>4</sub>) NPs (<100 nm) at different concentrations (1, 5 and 10 mg/L) to the culture media on several morphological, physiological and -biochemical parameters of in vitro shoot cultures of <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales (Lamiaceae), as well as on phenolic profile and bioactivity (antioxidant and enzyme inhibition capacities). Although some decreases in shoot number and length were observed in response to NPs, biomass production was not affected or was improved in both species. Most NPs treatments decreased total chlorophyll and carotenoid contents and increased malondialdehyde levels, an indicator of lipid peroxidation, in both species. HPLC-HR-MS analysis led to the identification of thirteen and twelve phenolic compounds, respectively, in <i>L. viridis</i> and <i>T. lotocephalus</i> extracts, being rosmarinic acid the major compound found in all the extracts. ZnO and Fe<sub>3</sub>O<sub>4</sub> NPs induced an increase in total phenolic and rosmarinic acid contents in <i>T. lotocephalus</i> extracts. Additionally, some NPs treatments also increased antioxidant activity in extracts from this species and the opposite was observed for <i>L. viridis</i>. The capacity of the extracts to inhibit tyrosinase, acetylcholinesterase and butyrylcholinesterase enzymes was not considerably affected. Overall, NPs had a significant impact on different parameters of <i>L. viridis</i> and <i>T. lotocephalus</i> in vitro shoot cultures, although the results varied with the species and NPs type.
format	article
author	Sandra Gonçalves Inês Mansinhos Raquel Rodríguez-Solana Gema Pereira-Caro José Manuel Moreno-Rojas Anabela Romano
author_facet	Sandra Gonçalves Inês Mansinhos Raquel Rodríguez-Solana Gema Pereira-Caro José Manuel Moreno-Rojas Anabela Romano
author_sort	Sandra Gonçalves
title	Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
title_short	Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
title_full	Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
title_fullStr	Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
title_full_unstemmed	Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales
title_sort	impact of metallic nanoparticles on in vitro culture, phenolic profile and biological activity of two mediterranean lamiaceae species: <i>lavandula viridis</i> l’hér and <i>thymus lotocephalus</i> g. lópez and r. morales
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/844147f39019464380f866b1dd728110
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Impact of Metallic Nanoparticles on In Vitro Culture, Phenolic Profile and Biological Activity of Two Mediterranean Lamiaceae Species: <i>Lavandula viridis</i> L’Hér and <i>Thymus lotocephalus</i> G. López and R. Morales

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