Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.

Animal embryonic stem cells (ESCs) provide powerful tool for studies of early embryonic development, gene targeting, cloning, and regenerative medicine. However, the majority of attempts to establish ESC lines from large animals, especially ungulate mammals have failed. Recently, another type of plu...

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Autores principales:	Yang Li, Ming Cang, Andrew Stephen Lee, Kehua Zhang, Dongjun Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8447742c38be4b349fa234d7ecb32f2e
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spelling	oai:doaj.org-article:8447742c38be4b349fa234d7ecb32f2e2021-11-18T07:00:42ZReprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.1932-620310.1371/journal.pone.0015947https://doaj.org/article/8447742c38be4b349fa234d7ecb32f2e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21253598/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Animal embryonic stem cells (ESCs) provide powerful tool for studies of early embryonic development, gene targeting, cloning, and regenerative medicine. However, the majority of attempts to establish ESC lines from large animals, especially ungulate mammals have failed. Recently, another type of pluripotent stem cells, known as induced pluripotent stem cells (iPSCs), have been successfully generated from mouse, human, monkey, rat and pig. In this study we show sheep fibroblasts can be reprogrammed to pluripotency by defined factors using a drug-inducible system. Sheep iPSCs derived in this fashion have a normal karyotype, exhibit morphological features similar to those of human ESCs and express AP, Oct4, Sox2, Nanog and the cell surface marker SSEA-4. Pluripotency of these cells was further confirmed by embryoid body (EB) and teratoma formation assays which generated derivatives of all three germ layers. Our results also show that the substitution of knockout serum replacement (KSR) with fetal bovine serum in culture improves the reprogramming efficiency of sheep iPSCs. Generation of sheep iPSCs places sheep on the front lines of large animal preclinical trials and experiments involving modification of animal genomes.Yang LiMing CangAndrew Stephen LeeKehua ZhangDongjun LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 1, p e15947 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yang Li Ming Cang Andrew Stephen Lee Kehua Zhang Dongjun Liu Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
description	Animal embryonic stem cells (ESCs) provide powerful tool for studies of early embryonic development, gene targeting, cloning, and regenerative medicine. However, the majority of attempts to establish ESC lines from large animals, especially ungulate mammals have failed. Recently, another type of pluripotent stem cells, known as induced pluripotent stem cells (iPSCs), have been successfully generated from mouse, human, monkey, rat and pig. In this study we show sheep fibroblasts can be reprogrammed to pluripotency by defined factors using a drug-inducible system. Sheep iPSCs derived in this fashion have a normal karyotype, exhibit morphological features similar to those of human ESCs and express AP, Oct4, Sox2, Nanog and the cell surface marker SSEA-4. Pluripotency of these cells was further confirmed by embryoid body (EB) and teratoma formation assays which generated derivatives of all three germ layers. Our results also show that the substitution of knockout serum replacement (KSR) with fetal bovine serum in culture improves the reprogramming efficiency of sheep iPSCs. Generation of sheep iPSCs places sheep on the front lines of large animal preclinical trials and experiments involving modification of animal genomes.
format	article
author	Yang Li Ming Cang Andrew Stephen Lee Kehua Zhang Dongjun Liu
author_facet	Yang Li Ming Cang Andrew Stephen Lee Kehua Zhang Dongjun Liu
author_sort	Yang Li
title	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
title_short	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
title_full	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
title_fullStr	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
title_full_unstemmed	Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
title_sort	reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/8447742c38be4b349fa234d7ecb32f2e
work_keys_str_mv	AT yangli reprogrammingofsheepfibroblastsintopluripotencyunderadruginducibleexpressionofmousederiveddefinedfactors AT mingcang reprogrammingofsheepfibroblastsintopluripotencyunderadruginducibleexpressionofmousederiveddefinedfactors AT andrewstephenlee reprogrammingofsheepfibroblastsintopluripotencyunderadruginducibleexpressionofmousederiveddefinedfactors AT kehuazhang reprogrammingofsheepfibroblastsintopluripotencyunderadruginducibleexpressionofmousederiveddefinedfactors AT dongjunliu reprogrammingofsheepfibroblastsintopluripotencyunderadruginducibleexpressionofmousederiveddefinedfactors
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Reprogramming of sheep fibroblasts into pluripotency under a drug-inducible expression of mouse-derived defined factors.

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