Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model

High carbohydrate influences the cartilage microcellular environment and chondrocytes. Perlecan (HSPG2) conducts an essential role as chondrocytes mechano-transducer and chondrocytes secretion factors. This research aims to fulfill the research gap about hyperglycemia which influences to perlecan of...

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Autores principales:	Ibrahim Njoto, Fatchiyah Fatchiyah, Kusworini Handono, Abdurrachman Abdurrachman, Djoko W. Soeatmadji, Handono Kalim
Formato:	article
Lenguaje:	EN
Publicado:	University of Brawijaya 2019
Materias:	anabolic catabolic chondrocyte hyperglycemia perlecan Chemistry QD1-999
Acceso en línea:	http://dx.doi.org/10.21776/ub.jpacr.2019.008.01.450 https://doaj.org/article/844afa7814a040c79d48bc494e233588
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spelling	oai:doaj.org-article:844afa7814a040c79d48bc494e2335882021-12-02T11:42:02ZModulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Modelhttp://dx.doi.org/10.21776/ub.jpacr.2019.008.01.4502302-46902302-4690https://doaj.org/article/844afa7814a040c79d48bc494e2335882019-04-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/450/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2302-4690High carbohydrate influences the cartilage microcellular environment and chondrocytes. Perlecan (HSPG2) conducts an essential role as chondrocytes mechano-transducer and chondrocytes secretion factors. This research aims to fulfill the research gap about hyperglycemia which influences to perlecan of articular cartilage. About twenty male rats were divided into four groups: group I (sugar-treated rats 1.00 mg/kg of BW), group II (sugar-treated rats 1.5 mg/kg of BW), group III (2.00 mg/kg of BW), and control. The Anterior Cruciate Ligament Transection (ACLT) used to generate an unstable joint to be osteoarthritis condition at the right knee. Then, sugar was administrated for two months. Level expression of the protein was analyzed using Western Blotting. The result showed that perlecan expression was decreased within all sugar-treated rats group as compared to control. The IGF-1 expression was decreased, whereas TGF-β was slightly increased within all treatment groups. Moreover, the expression of FGF-2 was higher in all treatment groups. Of note, IL-1β expression was only elevated in group II and III. The interaction of perlecan to the chondrocyte secretion factors was determined by the cartilage condition within hyperglycemia. Ibrahim Njoto Fatchiyah FatchiyahKusworini HandonoAbdurrachman AbdurrachmanDjoko W. SoeatmadjiHandono KalimUniversity of BrawijayaarticleanaboliccatabolicchondrocytehyperglycemiaperlecanChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 8, Iss 1, Pp 80-86 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	anabolic catabolic chondrocyte hyperglycemia perlecan Chemistry QD1-999
spellingShingle	anabolic catabolic chondrocyte hyperglycemia perlecan Chemistry QD1-999 Ibrahim Njoto Fatchiyah Fatchiyah Kusworini Handono Abdurrachman Abdurrachman Djoko W. Soeatmadji Handono Kalim Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
description	High carbohydrate influences the cartilage microcellular environment and chondrocytes. Perlecan (HSPG2) conducts an essential role as chondrocytes mechano-transducer and chondrocytes secretion factors. This research aims to fulfill the research gap about hyperglycemia which influences to perlecan of articular cartilage. About twenty male rats were divided into four groups: group I (sugar-treated rats 1.00 mg/kg of BW), group II (sugar-treated rats 1.5 mg/kg of BW), group III (2.00 mg/kg of BW), and control. The Anterior Cruciate Ligament Transection (ACLT) used to generate an unstable joint to be osteoarthritis condition at the right knee. Then, sugar was administrated for two months. Level expression of the protein was analyzed using Western Blotting. The result showed that perlecan expression was decreased within all sugar-treated rats group as compared to control. The IGF-1 expression was decreased, whereas TGF-β was slightly increased within all treatment groups. Moreover, the expression of FGF-2 was higher in all treatment groups. Of note, IL-1β expression was only elevated in group II and III. The interaction of perlecan to the chondrocyte secretion factors was determined by the cartilage condition within hyperglycemia.
format	article
author	Ibrahim Njoto Fatchiyah Fatchiyah Kusworini Handono Abdurrachman Abdurrachman Djoko W. Soeatmadji Handono Kalim
author_facet	Ibrahim Njoto Fatchiyah Fatchiyah Kusworini Handono Abdurrachman Abdurrachman Djoko W. Soeatmadji Handono Kalim
author_sort	Ibrahim Njoto
title	Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
title_short	Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
title_full	Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
title_fullStr	Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
title_full_unstemmed	Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model
title_sort	modulation of perlecan protein towards chondrocyte secretion factors at the articular cartilage in hyperglycemic animal model
publisher	University of Brawijaya
publishDate	2019
url	http://dx.doi.org/10.21776/ub.jpacr.2019.008.01.450 https://doaj.org/article/844afa7814a040c79d48bc494e233588
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_version_	1718395352986419200

Modulation of Perlecan Protein towards Chondrocyte Secretion Factors at the Articular Cartilage in Hyperglycemic Animal Model

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