A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)

Abstract Successful commercialization of wearable diagnostic sensors necessitates stability in detection of analytes over prolonged and continuous exposure to sweat. Challenges are primarily in ensuring target disease specific small analytes (i.e. metabolites, proteins, etc.) stability in complex sw...

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Autores principales:	Rujuta D. Munje, Sriram Muthukumar, Badrinath Jagannath, Shalini Prasad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8450c10bcd4b42158b77689500128c04
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spelling	oai:doaj.org-article:8450c10bcd4b42158b77689500128c042021-12-02T12:31:54ZA new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)10.1038/s41598-017-02133-02045-2322https://doaj.org/article/8450c10bcd4b42158b77689500128c042017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02133-0https://doaj.org/toc/2045-2322Abstract Successful commercialization of wearable diagnostic sensors necessitates stability in detection of analytes over prolonged and continuous exposure to sweat. Challenges are primarily in ensuring target disease specific small analytes (i.e. metabolites, proteins, etc.) stability in complex sweat buffer with varying pH levels and composition over time. We present a facile approach to address these challenges using RTILs with antibody functionalized sensors on nanoporous, flexible polymer membranes. Temporal studies were performed using both infrared spectroscopic, dynamic light scattering, and impedimetric spectroscopy to demonstrate stability in detection of analytes, Interleukin-6 (IL-6) and Cortisol, from human sweat in RTILs. Temporal stability in sensor performance was performed as follows: (a) detection of target analytes after 0, 24, 48, 96, and 168 hours post-antibody sensor functionalization; and (b) continuous detection of target analytes post-antibody sensor functionalization. Limit of detection of IL-6 in human sweat was 0.2 pg/mL for 0–24 hours and 2 pg/mL for 24–48 hours post-antibody sensor functionalization. Continuous detection of IL-6 over 0.2–200 pg/mL in human sweat was demonstrated for a period of 10 hours post-antibody sensor functionalization. Furthermore, combinatorial detection of IL-6 and Cortisol in human sweat was established with minimal cross-talk for 0–48 hours post-antibody sensor functionalization.Rujuta D. MunjeSriram MuthukumarBadrinath JagannathShalini PrasadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rujuta D. Munje Sriram Muthukumar Badrinath Jagannath Shalini Prasad A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
description	Abstract Successful commercialization of wearable diagnostic sensors necessitates stability in detection of analytes over prolonged and continuous exposure to sweat. Challenges are primarily in ensuring target disease specific small analytes (i.e. metabolites, proteins, etc.) stability in complex sweat buffer with varying pH levels and composition over time. We present a facile approach to address these challenges using RTILs with antibody functionalized sensors on nanoporous, flexible polymer membranes. Temporal studies were performed using both infrared spectroscopic, dynamic light scattering, and impedimetric spectroscopy to demonstrate stability in detection of analytes, Interleukin-6 (IL-6) and Cortisol, from human sweat in RTILs. Temporal stability in sensor performance was performed as follows: (a) detection of target analytes after 0, 24, 48, 96, and 168 hours post-antibody sensor functionalization; and (b) continuous detection of target analytes post-antibody sensor functionalization. Limit of detection of IL-6 in human sweat was 0.2 pg/mL for 0–24 hours and 2 pg/mL for 24–48 hours post-antibody sensor functionalization. Continuous detection of IL-6 over 0.2–200 pg/mL in human sweat was demonstrated for a period of 10 hours post-antibody sensor functionalization. Furthermore, combinatorial detection of IL-6 and Cortisol in human sweat was established with minimal cross-talk for 0–48 hours post-antibody sensor functionalization.
format	article
author	Rujuta D. Munje Sriram Muthukumar Badrinath Jagannath Shalini Prasad
author_facet	Rujuta D. Munje Sriram Muthukumar Badrinath Jagannath Shalini Prasad
author_sort	Rujuta D. Munje
title	A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
title_short	A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
title_full	A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
title_fullStr	A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
title_full_unstemmed	A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)
title_sort	new paradigm in sweat based wearable diagnostics biosensors using room temperature ionic liquids (rtils)
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/8450c10bcd4b42158b77689500128c04
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A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs)

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