Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis

Abstract Islets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss of islets in the process of isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, the optimization of culture plays a decisive role in the transplantation suc...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Somayeh Keshtkar, Maryam Kaviani, Zahra Jabbarpour, Bita Geramizadeh, Elahe Motevaseli, Saman Nikeghbalian, Alireza Shamsaeefar, Nasrin Motazedian, Ismail H. Al-Abdullah, Mohammad Hossein Ghahremani, Negar Azarpira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/8452747042f54cf090beddaab0c7fd41
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:8452747042f54cf090beddaab0c7fd41
record_format	dspace
spelling	oai:doaj.org-article:8452747042f54cf090beddaab0c7fd412021-12-02T16:08:52ZProtective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis10.1038/s41598-019-48262-62045-2322https://doaj.org/article/8452747042f54cf090beddaab0c7fd412019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48262-6https://doaj.org/toc/2045-2322Abstract Islets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss of islets in the process of isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, the optimization of culture plays a decisive role in the transplantation success. In this study, we evaluated the effect of nobiletin on the cultured human islets. Isolated human islets were treated by different concentrations of nobiletin and cultured for 24 and 72 hours. Then, the islets viability, apoptosis, insulin and C-peptide secretion, and apoptosis markers were evaluated. Also, the production of reactive oxygen species (ROS), hypoxia inducible factor 1 alpha (HIF-1α), and its target genes in the islets were examined. Our findings showed that the islets were encountered with hypoxia and oxidative stress after isolation and during culture. These insults induced apoptosis and reduced viability during culture period. Moreover, the secretion of insulin and C-peptide decreased. Nobiletin treatments significantly improved the islets survival through reduction of HIF-1α and ROS production and suppression of apoptosis, along with increased islets function. Islet protective effect of nobiletin might be related to its anti-oxidant, anti-apoptotic and insulinotropic properties. Hence, in order to achieve viable and functional islets for clinical transplantation, the application of nobiletin during pre-transplantation period is useful.Somayeh KeshtkarMaryam KavianiZahra JabbarpourBita GeramizadehElahe MotevaseliSaman NikeghbalianAlireza ShamsaeefarNasrin MotazedianIsmail H. Al-AbdullahMohammad Hossein GhahremaniNegar AzarpiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-13 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Somayeh Keshtkar Maryam Kaviani Zahra Jabbarpour Bita Geramizadeh Elahe Motevaseli Saman Nikeghbalian Alireza Shamsaeefar Nasrin Motazedian Ismail H. Al-Abdullah Mohammad Hossein Ghahremani Negar Azarpira Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
description	Abstract Islets transplantation, as a treatment of type 1 diabetes, faces challenges, including the loss of islets in the process of isolation and pre-transplantation due to cellular stresses-induced apoptosis. Accordingly, the optimization of culture plays a decisive role in the transplantation success. In this study, we evaluated the effect of nobiletin on the cultured human islets. Isolated human islets were treated by different concentrations of nobiletin and cultured for 24 and 72 hours. Then, the islets viability, apoptosis, insulin and C-peptide secretion, and apoptosis markers were evaluated. Also, the production of reactive oxygen species (ROS), hypoxia inducible factor 1 alpha (HIF-1α), and its target genes in the islets were examined. Our findings showed that the islets were encountered with hypoxia and oxidative stress after isolation and during culture. These insults induced apoptosis and reduced viability during culture period. Moreover, the secretion of insulin and C-peptide decreased. Nobiletin treatments significantly improved the islets survival through reduction of HIF-1α and ROS production and suppression of apoptosis, along with increased islets function. Islet protective effect of nobiletin might be related to its anti-oxidant, anti-apoptotic and insulinotropic properties. Hence, in order to achieve viable and functional islets for clinical transplantation, the application of nobiletin during pre-transplantation period is useful.
format	article
author	Somayeh Keshtkar Maryam Kaviani Zahra Jabbarpour Bita Geramizadeh Elahe Motevaseli Saman Nikeghbalian Alireza Shamsaeefar Nasrin Motazedian Ismail H. Al-Abdullah Mohammad Hossein Ghahremani Negar Azarpira
author_facet	Somayeh Keshtkar Maryam Kaviani Zahra Jabbarpour Bita Geramizadeh Elahe Motevaseli Saman Nikeghbalian Alireza Shamsaeefar Nasrin Motazedian Ismail H. Al-Abdullah Mohammad Hossein Ghahremani Negar Azarpira
author_sort	Somayeh Keshtkar
title	Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
title_short	Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
title_full	Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
title_fullStr	Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
title_full_unstemmed	Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
title_sort	protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/8452747042f54cf090beddaab0c7fd41
work_keys_str_mv	AT somayehkeshtkar protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT maryamkaviani protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT zahrajabbarpour protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT bitageramizadeh protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT elahemotevaseli protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT samannikeghbalian protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT alirezashamsaeefar protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT nasrinmotazedian protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT ismailhalabdullah protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT mohammadhosseinghahremani protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis AT negarazarpira protectiveeffectofnobiletinonisolatedhumanisletssurvivalandfunctionagainsthypoxiaandoxidativestressinducedapoptosis
_version_	1718384481986936832

Protective effect of nobiletin on isolated human islets survival and function against hypoxia and oxidative stress-induced apoptosis

Ejemplares similares