Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.
<h4>Background</h4>IL-33, a member of the IL-1 family of cytokines, provokes Th2-type inflammation accompanied by accumulation of eosinophils through IL-33R, which consists of ST2 and IL-1RAcP. We previously demonstrated that macrophages produce IL-33 in response to LPS. Some immune resp...
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2011
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oai:doaj.org-article:84558daedbd3469eb3317bb929bb70a92021-11-18T06:55:59ZParacrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation.1932-620310.1371/journal.pone.0018404https://doaj.org/article/84558daedbd3469eb3317bb929bb70a92011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21494550/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>IL-33, a member of the IL-1 family of cytokines, provokes Th2-type inflammation accompanied by accumulation of eosinophils through IL-33R, which consists of ST2 and IL-1RAcP. We previously demonstrated that macrophages produce IL-33 in response to LPS. Some immune responses were shown to differ between ST2-deficient mice and soluble ST2-Fc fusion protein-treated mice. Even in anti-ST2 antibody (Ab)-treated mice, the phenotypes differed between distinct Ab clones, because the characterization of such Abs (i.e., depletion, agonistic or blocking Abs) was unclear in some cases.<h4>Methodology/principal findings</h4>To elucidate the precise role of IL-33, we newly generated neutralizing monoclonal Abs for IL-33. Exogenous IL-33 potentiated LPS-mediated cytokine production by macrophages. That LPS-mediated cytokine production by macrophages was suppressed by inhibition of endogenous IL-33 by the anti-IL-33 neutralizing mAbs.<h4>Conclusions/significance</h4>Our findings suggest that LPS-mediated macrophage activation is accelerated by macrophage-derived paracrine IL-33 stimulation.Tatsukuni OhnoKeisuke ObokiHideaki MoritaNaoki KajiwaraKen AraeShizuko TanakaMasako IkedaMotoyasu IikuraTaishin AkiyamaJun-ichiro InoueKenji MatsumotoKatsuko SudoMiyuki AzumaKo OkumuraThomas KamradtHirohisa SaitoSusumu NakaePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18404 (2011) |
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Medicine R Science Q Tatsukuni Ohno Keisuke Oboki Hideaki Morita Naoki Kajiwara Ken Arae Shizuko Tanaka Masako Ikeda Motoyasu Iikura Taishin Akiyama Jun-ichiro Inoue Kenji Matsumoto Katsuko Sudo Miyuki Azuma Ko Okumura Thomas Kamradt Hirohisa Saito Susumu Nakae Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| description |
<h4>Background</h4>IL-33, a member of the IL-1 family of cytokines, provokes Th2-type inflammation accompanied by accumulation of eosinophils through IL-33R, which consists of ST2 and IL-1RAcP. We previously demonstrated that macrophages produce IL-33 in response to LPS. Some immune responses were shown to differ between ST2-deficient mice and soluble ST2-Fc fusion protein-treated mice. Even in anti-ST2 antibody (Ab)-treated mice, the phenotypes differed between distinct Ab clones, because the characterization of such Abs (i.e., depletion, agonistic or blocking Abs) was unclear in some cases.<h4>Methodology/principal findings</h4>To elucidate the precise role of IL-33, we newly generated neutralizing monoclonal Abs for IL-33. Exogenous IL-33 potentiated LPS-mediated cytokine production by macrophages. That LPS-mediated cytokine production by macrophages was suppressed by inhibition of endogenous IL-33 by the anti-IL-33 neutralizing mAbs.<h4>Conclusions/significance</h4>Our findings suggest that LPS-mediated macrophage activation is accelerated by macrophage-derived paracrine IL-33 stimulation. |
| format |
article |
| author |
Tatsukuni Ohno Keisuke Oboki Hideaki Morita Naoki Kajiwara Ken Arae Shizuko Tanaka Masako Ikeda Motoyasu Iikura Taishin Akiyama Jun-ichiro Inoue Kenji Matsumoto Katsuko Sudo Miyuki Azuma Ko Okumura Thomas Kamradt Hirohisa Saito Susumu Nakae |
| author_facet |
Tatsukuni Ohno Keisuke Oboki Hideaki Morita Naoki Kajiwara Ken Arae Shizuko Tanaka Masako Ikeda Motoyasu Iikura Taishin Akiyama Jun-ichiro Inoue Kenji Matsumoto Katsuko Sudo Miyuki Azuma Ko Okumura Thomas Kamradt Hirohisa Saito Susumu Nakae |
| author_sort |
Tatsukuni Ohno |
| title |
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| title_short |
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| title_full |
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| title_fullStr |
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| title_full_unstemmed |
Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| title_sort |
paracrine il-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/84558daedbd3469eb3317bb929bb70a9 |
| work_keys_str_mv |
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