The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.

<h4>Background</h4>Lung inflammation and impaired alveolarization are hallmarks of bronchopulmonary dysplasia (BPD). We hypothesize that human amnion epithelial cells (hAECs) are anti-inflammatory and reduce lung injury in preterm lambs born after antenatal exposure to inflammation.<h...

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Autores principales:	Paris Clarice Papagianis, Siavash Ahmadi-Noorbakhsh, Rebecca Lim, Euan Wallace, Graeme Polglase, J Jane Pillow, Timothy J Moss
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/845aca10f07f4511a1f01a1e235956e5
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spelling	oai:doaj.org-article:845aca10f07f4511a1f01a1e235956e52021-12-02T20:09:59ZThe effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.1932-620310.1371/journal.pone.0253456https://doaj.org/article/845aca10f07f4511a1f01a1e235956e52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0253456https://doaj.org/toc/1932-6203<h4>Background</h4>Lung inflammation and impaired alveolarization are hallmarks of bronchopulmonary dysplasia (BPD). We hypothesize that human amnion epithelial cells (hAECs) are anti-inflammatory and reduce lung injury in preterm lambs born after antenatal exposure to inflammation.<h4>Methods</h4>Pregnant ewes received either intra-amniotic lipopolysaccharide (LPS, from E.coli 055:B5; 4mg) or saline (Sal) on day 126 of gestation. Lambs were delivered by cesarean section at 128 d gestation (term ~150 d). Lambs received intravenous hAECs (LPS/hAECs: n = 7; 30x106 cells) or equivalent volumes of saline (LPS/Sal, n = 10; or Sal/Sal, n = 9) immediately after birth. Respiratory support was gradually de-escalated, aimed at early weaning from mechanical ventilation towards unassisted respiration. Lung tissue was collected 1 week after birth. Lung morphology was assessed and mRNA levels for inflammatory mediators were measured.<h4>Results</h4>Respiratory support required by LPS/hAEC lambs was not different to Sal/Sal or LPS/Sal lambs. Lung tissue:airspace ratio was lower in the LPS/Sal compared to Sal/Sal lambs (P<0.05), but not LPS/hAEC lambs. LPS/hAEC lambs tended to have increased septation in their lungs versus LPS/Sal (P = 0.08). Expression of inflammatory cytokines was highest in LPS/hAECs lambs.<h4>Conclusions</h4>Postnatal administration of a single dose of hAECs stimulates a pulmonary immune response without changing ventilator requirements in preterm lambs born after intrauterine inflammation.Paris Clarice PapagianisSiavash Ahmadi-NoorbakhshRebecca LimEuan WallaceGraeme PolglaseJ Jane PillowTimothy J MossPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0253456 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Paris Clarice Papagianis Siavash Ahmadi-Noorbakhsh Rebecca Lim Euan Wallace Graeme Polglase J Jane Pillow Timothy J Moss The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
description	<h4>Background</h4>Lung inflammation and impaired alveolarization are hallmarks of bronchopulmonary dysplasia (BPD). We hypothesize that human amnion epithelial cells (hAECs) are anti-inflammatory and reduce lung injury in preterm lambs born after antenatal exposure to inflammation.<h4>Methods</h4>Pregnant ewes received either intra-amniotic lipopolysaccharide (LPS, from E.coli 055:B5; 4mg) or saline (Sal) on day 126 of gestation. Lambs were delivered by cesarean section at 128 d gestation (term ~150 d). Lambs received intravenous hAECs (LPS/hAECs: n = 7; 30x106 cells) or equivalent volumes of saline (LPS/Sal, n = 10; or Sal/Sal, n = 9) immediately after birth. Respiratory support was gradually de-escalated, aimed at early weaning from mechanical ventilation towards unassisted respiration. Lung tissue was collected 1 week after birth. Lung morphology was assessed and mRNA levels for inflammatory mediators were measured.<h4>Results</h4>Respiratory support required by LPS/hAEC lambs was not different to Sal/Sal or LPS/Sal lambs. Lung tissue:airspace ratio was lower in the LPS/Sal compared to Sal/Sal lambs (P<0.05), but not LPS/hAEC lambs. LPS/hAEC lambs tended to have increased septation in their lungs versus LPS/Sal (P = 0.08). Expression of inflammatory cytokines was highest in LPS/hAECs lambs.<h4>Conclusions</h4>Postnatal administration of a single dose of hAECs stimulates a pulmonary immune response without changing ventilator requirements in preterm lambs born after intrauterine inflammation.
format	article
author	Paris Clarice Papagianis Siavash Ahmadi-Noorbakhsh Rebecca Lim Euan Wallace Graeme Polglase J Jane Pillow Timothy J Moss
author_facet	Paris Clarice Papagianis Siavash Ahmadi-Noorbakhsh Rebecca Lim Euan Wallace Graeme Polglase J Jane Pillow Timothy J Moss
author_sort	Paris Clarice Papagianis
title	The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
title_short	The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
title_full	The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
title_fullStr	The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
title_full_unstemmed	The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
title_sort	effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/845aca10f07f4511a1f01a1e235956e5
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The effect of human amnion epithelial cells on lung development and inflammation in preterm lambs exposed to antenatal inflammation.

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