Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells

Abstract Background Echinococcus multilocularis is the causative agent of human hepatic alveolar echinococcosis (AE). AE can cause damage to several organs, primarily the liver, and have severe outcomes, such as hepatic failure and encephalopathy. The main purpose of this study was to explore the in...

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Autores principales:	Deping Cao, Emad Shamsan, Bofan Jiang, Haining Fan, Yaogang Zhang, Mustafa Abdo Saif Dehwah
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Echinococcus multilocularis Hepatic stellate cell Hepatic fibrosis Protoscoleces Collagen-I Alpha-smooth muscle actin Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/845d01d4670c46b2b5534d148137d076
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spelling	oai:doaj.org-article:845d01d4670c46b2b5534d148137d0762021-12-05T12:17:04ZStructural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells10.1186/s13071-021-05037-11756-3305https://doaj.org/article/845d01d4670c46b2b5534d148137d0762021-12-01T00:00:00Zhttps://doi.org/10.1186/s13071-021-05037-1https://doaj.org/toc/1756-3305Abstract Background Echinococcus multilocularis is the causative agent of human hepatic alveolar echinococcosis (AE). AE can cause damage to several organs, primarily the liver, and have severe outcomes, such as hepatic failure and encephalopathy. The main purpose of this study was to explore the interactions between hepatic stellate cells (HSCs) and E. multilocularis protoscoleces (PSCs). The results of this study provide an experimental basis for further examination of the pathogenesis of hepatic fibrosis due to AE infection. Methods We investigated the role of Echinococcus multilocularis (Echinococcus genus) PSCs in hepatic fibrosis by examining structural changes and measuring hepatic fibrosis-related protein levels in cocultures of PSCs and human HSCs. Structural changes were detected by transmission electron microscopy (TEM), and levels of the hepatic fibrosis-related proteins collagen I (Col-I), alpha-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured by western blotting and enzyme-linked immunosorbent assay (ELISA). Results Under coculture (1) both PSCs and HSCs exhibited morphological changes, as observed by TEM; (2) Col-I, α-SMA, and OPN expression levels, which were determined by western blotting and ELISA, significantly increased after 3 days of incubation. Conclusions The results of this study provide insights into the molecular mechanisms of AE-induced hepatic fibrosis. Graphical abstractDeping CaoEmad ShamsanBofan JiangHaining FanYaogang ZhangMustafa Abdo Saif DehwahBMCarticleEchinococcus multilocularisHepatic stellate cellHepatic fibrosisProtoscolecesCollagen-IAlpha-smooth muscle actinInfectious and parasitic diseasesRC109-216ENParasites & Vectors, Vol 14, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Echinococcus multilocularis Hepatic stellate cell Hepatic fibrosis Protoscoleces Collagen-I Alpha-smooth muscle actin Infectious and parasitic diseases RC109-216
spellingShingle	Echinococcus multilocularis Hepatic stellate cell Hepatic fibrosis Protoscoleces Collagen-I Alpha-smooth muscle actin Infectious and parasitic diseases RC109-216 Deping Cao Emad Shamsan Bofan Jiang Haining Fan Yaogang Zhang Mustafa Abdo Saif Dehwah Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
description	Abstract Background Echinococcus multilocularis is the causative agent of human hepatic alveolar echinococcosis (AE). AE can cause damage to several organs, primarily the liver, and have severe outcomes, such as hepatic failure and encephalopathy. The main purpose of this study was to explore the interactions between hepatic stellate cells (HSCs) and E. multilocularis protoscoleces (PSCs). The results of this study provide an experimental basis for further examination of the pathogenesis of hepatic fibrosis due to AE infection. Methods We investigated the role of Echinococcus multilocularis (Echinococcus genus) PSCs in hepatic fibrosis by examining structural changes and measuring hepatic fibrosis-related protein levels in cocultures of PSCs and human HSCs. Structural changes were detected by transmission electron microscopy (TEM), and levels of the hepatic fibrosis-related proteins collagen I (Col-I), alpha-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured by western blotting and enzyme-linked immunosorbent assay (ELISA). Results Under coculture (1) both PSCs and HSCs exhibited morphological changes, as observed by TEM; (2) Col-I, α-SMA, and OPN expression levels, which were determined by western blotting and ELISA, significantly increased after 3 days of incubation. Conclusions The results of this study provide insights into the molecular mechanisms of AE-induced hepatic fibrosis. Graphical abstract
format	article
author	Deping Cao Emad Shamsan Bofan Jiang Haining Fan Yaogang Zhang Mustafa Abdo Saif Dehwah
author_facet	Deping Cao Emad Shamsan Bofan Jiang Haining Fan Yaogang Zhang Mustafa Abdo Saif Dehwah
author_sort	Deping Cao
title	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
title_short	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
title_full	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
title_fullStr	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
title_full_unstemmed	Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells
title_sort	structural changes and expression of hepatic fibrosis-related proteins in coculture of echinococcus multilocularis protoscoleces and human hepatic stellate cells
publisher	BMC
publishDate	2021
url	https://doaj.org/article/845d01d4670c46b2b5534d148137d076
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Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells

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