Distribution and molecular characterization of biosurfactant-producing bacteria

Abstract. Alyousif NA, Luaibi YYYA, Hussein W. 2020. Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas 21: 4034-4040. Biosurfactants (BSs) are biological surface-active compounds produced by several microorganisms with many areas of application, as such b...

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Autores principales:	Nassir Alyousif, YASIN Y.Y. AL LUAIBI, WIJDAN HUSSEIN
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna gene sequencing, biosurfactant-producing bacteria, emulsification activity Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/845f09098a4d480ab9851ce79c985bd8
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spelling	oai:doaj.org-article:845f09098a4d480ab9851ce79c985bd82021-11-22T00:41:43ZDistribution and molecular characterization of biosurfactant-producing bacteria1412-033X2085-472210.13057/biodiv/d210914https://doaj.org/article/845f09098a4d480ab9851ce79c985bd82020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6341https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Alyousif NA, Luaibi YYYA, Hussein W. 2020. Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas 21: 4034-4040. Biosurfactants (BSs) are biological surface-active compounds produced by several microorganisms with many areas of application, as such become an important product in biotechnology and consequence to be used in industries. In recent years, many researchers pay attention to BSs producers' microorganisms. The present study was aimed to isolate, identify, and screening BS producing bacteria from six various sites in two different cities in Iraq. Four samples were collected from four sites in Basrah governorate and the rest two samples from Al-Garraf oilfield in Thi-Qar governorate. A total of 33 different bacterial isolates were obtained, 20 out of the 33 were found to be biosurfactants producing isolates that detected through the emulsification index (E24%), oil spreading test, and emulsification activity. The isolated bacterial strains were more identified by 16S rRNA gene sequencing. The results showed that the biosurfactants producing isolates belonged to genera Bacillus, Pseudomonas, Enterobacter, Staphylococcus, Acinetobacter, and Aerococcus. Bacillus jeotgali and Aerococcus viridans are reporting as biosurfactant producing bacteria for the first time and Bacillus jeotgali is isolated for first time from crude oil of oilfield reservoir in this study in world. Moreover, six bacterial isolates were identified as new strains and deposited at NCBI Genbank under accession numbers MT261834 (Bacillus subtilis strain IRQNWYA3), MT261835 (Bacillus licheniformis strain IRQNWYB4), MT261836 (Pseudomonas stutzeri strain IRQNWYF2), MT261837 (Pseudomonas zhaodongensis strain IRQNWYF3), MT261838 (Pseudomonas sp. IRQNWYF4) and MT261839 (Bacillus licheniformis strain IRQNWYF5). A2 isolate that was identified as Pseudomonas aeruginosa has shown the highest values of emulsification activity and emulsification index (1.678±0.050 absorbance at 540 nm and 56.6% respectively) that show efficient potential of biosurfactant production. Phylogenetic tree was also constructed in this study based on 16S rRNA gene sequences of biosurfactant-producing bacteria to evaluate their close relationship and evolution between them.Nassir AlyousifYASIN Y.Y. AL LUAIBIWIJDAN HUSSEINMBI & UNS Soloarticle16s rrna gene sequencing, biosurfactant-producing bacteria, emulsification activityBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene sequencing, biosurfactant-producing bacteria, emulsification activity Biology (General) QH301-705.5
spellingShingle	16s rrna gene sequencing, biosurfactant-producing bacteria, emulsification activity Biology (General) QH301-705.5 Nassir Alyousif YASIN Y.Y. AL LUAIBI WIJDAN HUSSEIN Distribution and molecular characterization of biosurfactant-producing bacteria
description	Abstract. Alyousif NA, Luaibi YYYA, Hussein W. 2020. Distribution and molecular characterization of biosurfactant-producing bacteria. Biodiversitas 21: 4034-4040. Biosurfactants (BSs) are biological surface-active compounds produced by several microorganisms with many areas of application, as such become an important product in biotechnology and consequence to be used in industries. In recent years, many researchers pay attention to BSs producers' microorganisms. The present study was aimed to isolate, identify, and screening BS producing bacteria from six various sites in two different cities in Iraq. Four samples were collected from four sites in Basrah governorate and the rest two samples from Al-Garraf oilfield in Thi-Qar governorate. A total of 33 different bacterial isolates were obtained, 20 out of the 33 were found to be biosurfactants producing isolates that detected through the emulsification index (E24%), oil spreading test, and emulsification activity. The isolated bacterial strains were more identified by 16S rRNA gene sequencing. The results showed that the biosurfactants producing isolates belonged to genera Bacillus, Pseudomonas, Enterobacter, Staphylococcus, Acinetobacter, and Aerococcus. Bacillus jeotgali and Aerococcus viridans are reporting as biosurfactant producing bacteria for the first time and Bacillus jeotgali is isolated for first time from crude oil of oilfield reservoir in this study in world. Moreover, six bacterial isolates were identified as new strains and deposited at NCBI Genbank under accession numbers MT261834 (Bacillus subtilis strain IRQNWYA3), MT261835 (Bacillus licheniformis strain IRQNWYB4), MT261836 (Pseudomonas stutzeri strain IRQNWYF2), MT261837 (Pseudomonas zhaodongensis strain IRQNWYF3), MT261838 (Pseudomonas sp. IRQNWYF4) and MT261839 (Bacillus licheniformis strain IRQNWYF5). A2 isolate that was identified as Pseudomonas aeruginosa has shown the highest values of emulsification activity and emulsification index (1.678±0.050 absorbance at 540 nm and 56.6% respectively) that show efficient potential of biosurfactant production. Phylogenetic tree was also constructed in this study based on 16S rRNA gene sequences of biosurfactant-producing bacteria to evaluate their close relationship and evolution between them.
format	article
author	Nassir Alyousif YASIN Y.Y. AL LUAIBI WIJDAN HUSSEIN
author_facet	Nassir Alyousif YASIN Y.Y. AL LUAIBI WIJDAN HUSSEIN
author_sort	Nassir Alyousif
title	Distribution and molecular characterization of biosurfactant-producing bacteria
title_short	Distribution and molecular characterization of biosurfactant-producing bacteria
title_full	Distribution and molecular characterization of biosurfactant-producing bacteria
title_fullStr	Distribution and molecular characterization of biosurfactant-producing bacteria
title_full_unstemmed	Distribution and molecular characterization of biosurfactant-producing bacteria
title_sort	distribution and molecular characterization of biosurfactant-producing bacteria
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/845f09098a4d480ab9851ce79c985bd8
work_keys_str_mv	AT nassiralyousif distributionandmolecularcharacterizationofbiosurfactantproducingbacteria AT yasinyyalluaibi distributionandmolecularcharacterizationofbiosurfactantproducingbacteria AT wijdanhussein distributionandmolecularcharacterizationofbiosurfactantproducingbacteria
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Distribution and molecular characterization of biosurfactant-producing bacteria

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