Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows

Abstract Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages...

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Autores principales:	Vinícius da Silva Duarte, Roberto Sousa Dias, Andrew M. Kropinski, Stefano Campanaro, Laura Treu, Carolina Siqueira, Marcella Silva Vieira, Isabela da Silva Paes, Gabriele Rocha Santana, Franciele Martins, Josicelli Souza Crispim, André da Silva Xavier, Camila Geovana Ferro, Pedro M. P. Vidigal, Cynthia Canêdo da Silva, Sérgio Oliveira de Paula
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/846e369a1e1d41b1ae50b596ee86f4a7
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spelling	oai:doaj.org-article:846e369a1e1d41b1ae50b596ee86f4a72021-12-02T12:32:22ZGenomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows10.1038/s41598-018-24896-w2045-2322https://doaj.org/article/846e369a1e1d41b1ae50b596ee86f4a72018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-24896-whttps://doaj.org/toc/2045-2322Abstract Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.Vinícius da Silva DuarteRoberto Sousa DiasAndrew M. KropinskiStefano CampanaroLaura TreuCarolina SiqueiraMarcella Silva VieiraIsabela da Silva PaesGabriele Rocha SantanaFranciele MartinsJosicelli Souza CrispimAndré da Silva XavierCamila Geovana FerroPedro M. P. VidigalCynthia Canêdo da SilvaSérgio Oliveira de PaulaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vinícius da Silva Duarte Roberto Sousa Dias Andrew M. Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro M. P. Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
description	Abstract Bovine mastitis remains the main cause of economic losses for dairy farmers. Mammary pathogenic Escherichia coli (MPEC) is related to an acute mastitis and its treatment is still based on the use of antibiotics. In the era of antimicrobial resistance (AMR), bacterial viruses (bacteriophages) present as an efficient treatment or prophylactic option. However, this makes it essential that its genetic structure, stability and interaction with the host immune system be thoroughly characterized. The present study analyzed a novel, broad host-range anti-mastitis agent, the T4virus vB_EcoM-UFV13 in genomic terms, and its activity against a MPEC strain in an experimental E. coli-induced mastitis mouse model. 4,975 Single Nucleotide Polymorphisms (SNPs) were assigned between vB_EcoM-UFV13 and E. coli phage T4 genomes with high impact on coding sequences (CDS) (37.60%) for virion proteins. Phylogenetic trees and genome analysis supported a recent infection mix between vB_EcoM-UFV13 and Shigella phage Shfl2. After a viral stability evaluation (e.g pH and temperature), intramammary administration (MOI 10) resulted in a 10-fold reduction in bacterial load. Furthermore, pro-inflammatory cytokines, such as IL-6 and TNF-α, were observed after viral treatment. This work brings the whole characterization and immune response to vB_EcoM-UFV13, a biocontrol candidate for bovine mastitis.
format	article
author	Vinícius da Silva Duarte Roberto Sousa Dias Andrew M. Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro M. P. Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula
author_facet	Vinícius da Silva Duarte Roberto Sousa Dias Andrew M. Kropinski Stefano Campanaro Laura Treu Carolina Siqueira Marcella Silva Vieira Isabela da Silva Paes Gabriele Rocha Santana Franciele Martins Josicelli Souza Crispim André da Silva Xavier Camila Geovana Ferro Pedro M. P. Vidigal Cynthia Canêdo da Silva Sérgio Oliveira de Paula
author_sort	Vinícius da Silva Duarte
title	Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
title_short	Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
title_full	Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
title_fullStr	Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
title_full_unstemmed	Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows
title_sort	genomic analysis and immune response in a murine mastitis model of vb_ecom-ufv13, a potential biocontrol agent for use in dairy cows
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/846e369a1e1d41b1ae50b596ee86f4a7
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Genomic analysis and immune response in a murine mastitis model of vB_EcoM-UFV13, a potential biocontrol agent for use in dairy cows

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