Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan

Lily (genus Lilium) is one of the most important flowers for bulb and cut flower production in the world. Lilium rubellum Baker is a native species of Japan that exhibits early flowering, is pink coloured, and possesses a pleasant fragrance, which makes it an important genetic resource for breeding...

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Autores principales:	M. Castro, T. Nishikawa
Formato:	article
Lenguaje:	EN
Publicado:	Universidad Nacional Agraria La Molina 2019
Materias:	genetic diversity rapd marker lilium rubellum Agriculture (General) S1-972
Acceso en línea:	https://doaj.org/article/84703490992b46aa9796523abf8da6cc
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spelling	oai:doaj.org-article:84703490992b46aa9796523abf8da6cc2021-12-04T17:41:07ZGenetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan2616-447710.21704/pja.v3i3.1251https://doaj.org/article/84703490992b46aa9796523abf8da6cc2019-12-01T00:00:00Zhttps://revistas.lamolina.edu.pe/index.php/jpagronomy/article/view/1251https://doaj.org/toc/2616-4477Lily (genus Lilium) is one of the most important flowers for bulb and cut flower production in the world. Lilium rubellum Baker is a native species of Japan that exhibits early flowering, is pink coloured, and possesses a pleasant fragrance, which makes it an important genetic resource for breeding lilies. In this work, the genetic diversity of L. rubellum was studied among three natural populations located in Fukushima Prefecture, Japan, which have been designated as the Mt. Azuma, Nango and Atsushio-kano populations. A total of 31 accessions collected in 1997 were analysed using Random Amplified Polymorphic DNA (RAPD) markers. Eighteen decamer primers produced 98% polymorphic RAPD bands; furthermore, 11 of the 18 primers produced 10 or more polymorphic bands with a mean per primer Polymorphic Information Content of 0.382. Among the three populations, the mean Shannon index, Nei´s gene diversity and percentage of polymorphic loci were 0.2749, 0.4099 and 76.7%, respectively. These data revealed that there is a high genetic diversity within all the populations. Analysis of the three genetic diversity indices within populations showed that the Nango population had the highest genetic diversity, whereas the Atsushio-kano population had the lowest. An unweighted pair group method with arithmetic mean dendrogram based on Jaccard´s similarity coefficient was constructed, and the three populations of L. rubellum collected in the Fukushima prefecture were clearly differentiated at 0.61 similarity index. The Nango and Mt. Azuma populations were genetically closer than the Atsushio-kano population. The analysis of molecular variance showed a 29.53% variance among the populations. This study revealed that there is high genetic diversity within populations and moderate genetic diversity among the three natural populations of L. rubellum Baker in the Fukushima Prefecture of Japan.M. CastroT. NishikawaUniversidad Nacional Agraria La Molinaarticlegenetic diversityrapd markerlilium rubellumAgriculture (General)S1-972ENPeruvian Journal of Agronomy, Vol 3, Iss 3, Pp 112-119 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	genetic diversity rapd marker lilium rubellum Agriculture (General) S1-972
spellingShingle	genetic diversity rapd marker lilium rubellum Agriculture (General) S1-972 M. Castro T. Nishikawa Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
description	Lily (genus Lilium) is one of the most important flowers for bulb and cut flower production in the world. Lilium rubellum Baker is a native species of Japan that exhibits early flowering, is pink coloured, and possesses a pleasant fragrance, which makes it an important genetic resource for breeding lilies. In this work, the genetic diversity of L. rubellum was studied among three natural populations located in Fukushima Prefecture, Japan, which have been designated as the Mt. Azuma, Nango and Atsushio-kano populations. A total of 31 accessions collected in 1997 were analysed using Random Amplified Polymorphic DNA (RAPD) markers. Eighteen decamer primers produced 98% polymorphic RAPD bands; furthermore, 11 of the 18 primers produced 10 or more polymorphic bands with a mean per primer Polymorphic Information Content of 0.382. Among the three populations, the mean Shannon index, Nei´s gene diversity and percentage of polymorphic loci were 0.2749, 0.4099 and 76.7%, respectively. These data revealed that there is a high genetic diversity within all the populations. Analysis of the three genetic diversity indices within populations showed that the Nango population had the highest genetic diversity, whereas the Atsushio-kano population had the lowest. An unweighted pair group method with arithmetic mean dendrogram based on Jaccard´s similarity coefficient was constructed, and the three populations of L. rubellum collected in the Fukushima prefecture were clearly differentiated at 0.61 similarity index. The Nango and Mt. Azuma populations were genetically closer than the Atsushio-kano population. The analysis of molecular variance showed a 29.53% variance among the populations. This study revealed that there is high genetic diversity within populations and moderate genetic diversity among the three natural populations of L. rubellum Baker in the Fukushima Prefecture of Japan.
format	article
author	M. Castro T. Nishikawa
author_facet	M. Castro T. Nishikawa
author_sort	M. Castro
title	Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
title_short	Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
title_full	Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
title_fullStr	Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
title_full_unstemmed	Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan
title_sort	genetic variation of maiden lily (lilium rubellum baker) within and among three natural populations in fukushima prefecture in japan
publisher	Universidad Nacional Agraria La Molina
publishDate	2019
url	https://doaj.org/article/84703490992b46aa9796523abf8da6cc
work_keys_str_mv	AT mcastro geneticvariationofmaidenlilyliliumrubellumbakerwithinandamongthreenaturalpopulationsinfukushimaprefectureinjapan AT tnishikawa geneticvariationofmaidenlilyliliumrubellumbakerwithinandamongthreenaturalpopulationsinfukushimaprefectureinjapan
_version_	1718372683939315712

Genetic variation of maiden lily (Lilium rubellum Baker) within and among three natural populations in Fukushima Prefecture in Japan

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