Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.

We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutinati...

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Autores principales:	Saeed El-Ashram, Mahmoud E Hashad, G A Abdel-Alim, Taher Abdelhamid, Heba N Deif
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/847af87e87cc489ab60cc68a981c8efc
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spelling	oai:doaj.org-article:847af87e87cc489ab60cc68a981c8efc2021-12-02T20:09:16ZSeroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.1932-620310.1371/journal.pone.0254220https://doaj.org/article/847af87e87cc489ab60cc68a981c8efc2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254220https://doaj.org/toc/1932-6203We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutination examination, a serological test, indicated the prevalence of Mg and Ms infections of 10.9% and 13.2%, respectively. On 91 SPA test positive serum samples for either Mg or Ms, a passive hemagglutination/hemagglutination inhibition (HI) test was performed. The SPA and HI test findings were found to be comparable. On 90 SPA test positive samples, an ELISA was performed using commercial kits for Mg and Ms serodiagnosis. According to the ELISA data, only 83.33% and 18.88% of SPA test positive samples were confirmed as positive for Ms and Mg infections, respectively. The prevalence increased to 84.44% and 77.77%, respectively, when suspected samples were deemed positive.Saeed El-AshramMahmoud E HashadG A Abdel-AlimTaher AbdelhamidHeba N DeifPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254220 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Saeed El-Ashram Mahmoud E Hashad G A Abdel-Alim Taher Abdelhamid Heba N Deif Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
description	We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutination examination, a serological test, indicated the prevalence of Mg and Ms infections of 10.9% and 13.2%, respectively. On 91 SPA test positive serum samples for either Mg or Ms, a passive hemagglutination/hemagglutination inhibition (HI) test was performed. The SPA and HI test findings were found to be comparable. On 90 SPA test positive samples, an ELISA was performed using commercial kits for Mg and Ms serodiagnosis. According to the ELISA data, only 83.33% and 18.88% of SPA test positive samples were confirmed as positive for Ms and Mg infections, respectively. The prevalence increased to 84.44% and 77.77%, respectively, when suspected samples were deemed positive.
format	article
author	Saeed El-Ashram Mahmoud E Hashad G A Abdel-Alim Taher Abdelhamid Heba N Deif
author_facet	Saeed El-Ashram Mahmoud E Hashad G A Abdel-Alim Taher Abdelhamid Heba N Deif
author_sort	Saeed El-Ashram
title	Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
title_short	Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
title_full	Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
title_fullStr	Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
title_full_unstemmed	Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.
title_sort	seroprevalence of mycoplasmosis in broiler, layer, and native chickens in giza, egypt.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/847af87e87cc489ab60cc68a981c8efc
work_keys_str_mv	AT saeedelashram seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt AT mahmoudehashad seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt AT gaabdelalim seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt AT taherabdelhamid seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt AT hebandeif seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt
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Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt.

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