Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy

Dan Wang,1,2,* Xuefen Li,3,* Xinfang Li,4,* Anfeng Kang,4 Linhong Sun,4 Miao Sun,4 Feng Yang,4 Congjian Xu1 1Shanghai Key Laboratory of Female Reproductive Endocrine Related Disease, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People’s Republic of China; 2Department of...

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Autores principales:	Wang D, Li X, Kang A, Sun L, Sun M, Yang F, Xu C
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	magnetic mesoporous silica doxorubicin magnetic targeting pH responsive photodynamic therapy Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/847d3f71e96842f181cc5e48b737b535
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spelling	oai:doaj.org-article:847d3f71e96842f181cc5e48b737b5352021-12-02T08:18:08ZMagnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy1178-2013https://doaj.org/article/847d3f71e96842f181cc5e48b737b5352019-09-01T00:00:00Zhttps://www.dovepress.com/magnetic-and-ph-dual-responsive-nanoparticles-for-synergistic-drug-res-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Dan Wang,1,2,* Xuefen Li,3,* Xinfang Li,4,* Anfeng Kang,4 Linhong Sun,4 Miao Sun,4 Feng Yang,4 Congjian Xu1 1Shanghai Key Laboratory of Female Reproductive Endocrine Related Disease, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People’s Republic of China; 2Department of Gynaecology and Obstetrics, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 3Department of Nephrology, Jiulongpo People’s Hospital, Chongqing, People’s Republic of China; 4Inorganic Chemistry Department, School of Pharmacy, Second Military Medical University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feng YangInorganic Chemistry Department, School of Pharmacy, Second Military Medical University, Shanghai 200433, People’s Republic of ChinaTel +86 21 8187 1218Email yangfeng1008@126.comCongjian XuDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai 200011, People’s Republic of ChinaTel +86 21 33189900Email xucongjian@fudan.edu.cnBackground: Drug resistance is one of the prime reasons of chemotherapy failure in breast cancer and is also an important factor affecting prognosis.Purpose: In this study, we constructed a functional magnetic mesoporous silica-based nanocomposite (MMSN) for breast cancer chemotherapy/photodynamic therapy.Methods: MMSN was characterized by scanning electron microscopy and transmission electron microscopy to observe the morphology. The size distribution and zeta potential of the MSNs were determined using Malvern Particle Size Analyzer. Anti-tumor activity in vitro was investigated by CCK-8 assay, flow cytometry and transwell experiment, and the anti-tumor activity in vivo was probed into by magnetic targeting, toxicity, and antitumor effects in breast cancer-bearing BABL/c nude mice.Results: The results showed that the release of doxorubicin in the nanocomposites was pH sensitive, and the cumulative release rate reached 80.53% at 60 h under acidic conditions. The nanocomposites had a high cellular uptake ability in MCF-7/ADR cells, and the IC50 value of the nanocomposites on MCF-7/ADR cells was 4.23 μg/mL, much smaller than that of free DOX (363.2 μg/mL). The nanocomposites could effectively reverse resistance and induce apoptosis of MCF-7/ADR cells. The blood biochemistry parameters and H&E staining results showed no serious adverse effects after treatment with the nanocomposites. Prussian blue staining showed that the nanocomposites were able to target tumor tissues in tumor-bearing mice under a magnetic field. The combined chemical/photodynamic therapy significantly inhibited tumor growth in vivo.Conclusion: Nanocomposites with magnetic and pH dual-responsive performance has shown a promising platform for enhanced drug-resistant breast cancer treatment.Keywords: magnetic mesoporous silica, doxorubicin, magnetic targeting, pH responsive, photodynamic therapyWang DLi XLi XKang ASun LSun MYang FXu CDove Medical Pressarticlemagnetic mesoporous silicadoxorubicinmagnetic targetingpH responsivephotodynamic therapyMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 7665-7679 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic mesoporous silica doxorubicin magnetic targeting pH responsive photodynamic therapy Medicine (General) R5-920
spellingShingle	magnetic mesoporous silica doxorubicin magnetic targeting pH responsive photodynamic therapy Medicine (General) R5-920 Wang D Li X Li X Kang A Sun L Sun M Yang F Xu C Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
description	Dan Wang,1,2,* Xuefen Li,3,* Xinfang Li,4,* Anfeng Kang,4 Linhong Sun,4 Miao Sun,4 Feng Yang,4 Congjian Xu1 1Shanghai Key Laboratory of Female Reproductive Endocrine Related Disease, Obstetrics and Gynecology Hospital, Fudan University, Shanghai, People’s Republic of China; 2Department of Gynaecology and Obstetrics, Changzheng Hospital, Second Military Medical University, Shanghai, People’s Republic of China; 3Department of Nephrology, Jiulongpo People’s Hospital, Chongqing, People’s Republic of China; 4Inorganic Chemistry Department, School of Pharmacy, Second Military Medical University, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Feng YangInorganic Chemistry Department, School of Pharmacy, Second Military Medical University, Shanghai 200433, People’s Republic of ChinaTel +86 21 8187 1218Email yangfeng1008@126.comCongjian XuDepartment of Gynecology, Obstetrics and Gynecology Hospital, Fudan University, 419 Fangxie Road, Shanghai 200011, People’s Republic of ChinaTel +86 21 33189900Email xucongjian@fudan.edu.cnBackground: Drug resistance is one of the prime reasons of chemotherapy failure in breast cancer and is also an important factor affecting prognosis.Purpose: In this study, we constructed a functional magnetic mesoporous silica-based nanocomposite (MMSN) for breast cancer chemotherapy/photodynamic therapy.Methods: MMSN was characterized by scanning electron microscopy and transmission electron microscopy to observe the morphology. The size distribution and zeta potential of the MSNs were determined using Malvern Particle Size Analyzer. Anti-tumor activity in vitro was investigated by CCK-8 assay, flow cytometry and transwell experiment, and the anti-tumor activity in vivo was probed into by magnetic targeting, toxicity, and antitumor effects in breast cancer-bearing BABL/c nude mice.Results: The results showed that the release of doxorubicin in the nanocomposites was pH sensitive, and the cumulative release rate reached 80.53% at 60 h under acidic conditions. The nanocomposites had a high cellular uptake ability in MCF-7/ADR cells, and the IC50 value of the nanocomposites on MCF-7/ADR cells was 4.23 μg/mL, much smaller than that of free DOX (363.2 μg/mL). The nanocomposites could effectively reverse resistance and induce apoptosis of MCF-7/ADR cells. The blood biochemistry parameters and H&E staining results showed no serious adverse effects after treatment with the nanocomposites. Prussian blue staining showed that the nanocomposites were able to target tumor tissues in tumor-bearing mice under a magnetic field. The combined chemical/photodynamic therapy significantly inhibited tumor growth in vivo.Conclusion: Nanocomposites with magnetic and pH dual-responsive performance has shown a promising platform for enhanced drug-resistant breast cancer treatment.Keywords: magnetic mesoporous silica, doxorubicin, magnetic targeting, pH responsive, photodynamic therapy
format	article
author	Wang D Li X Li X Kang A Sun L Sun M Yang F Xu C
author_facet	Wang D Li X Li X Kang A Sun L Sun M Yang F Xu C
author_sort	Wang D
title	Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
title_short	Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
title_full	Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
title_fullStr	Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
title_full_unstemmed	Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy
title_sort	magnetic and ph dual-responsive nanoparticles for synergistic drug-resistant breast cancer chemo/photodynamic therapy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/847d3f71e96842f181cc5e48b737b535
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Magnetic And pH Dual-Responsive Nanoparticles For Synergistic Drug-Resistant Breast Cancer Chemo/Photodynamic Therapy

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