Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

Hongyu Sun,* Jing Zhou,* Zhu Huang,* Linlin Qu,* Ning Lin,* Chengxiao Liang, Ruiwu Dai, Lijun Tang, Fuzhou Tian General Surgery Center, Chengdu Military General Hospital, Chengdu, China *These authors contributed equally to this work Abstract: Carbon nanotubes (CNTs) provide an essential 2-D micr...

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Autores principales:	Sun HY, Zhou J, Huang Z, Qu LL, Lin N, Liang CX, Dai RW, Tang LJ, Tian FZ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	carbon nanotubes collagen hydrogel cardiac constructs cell alignment tissue functionality Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/847e9403607e4cb191ecf3a42ae55b7e
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spelling	oai:doaj.org-article:847e9403607e4cb191ecf3a42ae55b7e2021-12-02T01:49:54ZCarbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs1178-2013https://doaj.org/article/847e9403607e4cb191ecf3a42ae55b7e2017-04-01T00:00:00Zhttps://www.dovepress.com/carbon-nanotube-incorporated-collagen-hydrogels-improve-cell-alignment-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Hongyu Sun,* Jing Zhou,* Zhu Huang,* Linlin Qu,* Ning Lin,* Chengxiao Liang, Ruiwu Dai, Lijun Tang, Fuzhou Tian General Surgery Center, Chengdu Military General Hospital, Chengdu, China *These authors contributed equally to this work Abstract: Carbon nanotubes (CNTs) provide an essential 2-D microenvironment for cardiomyocyte growth and function. However, it remains to be elucidated whether CNT nanostructures can promote cell–cell integrity and facilitate the formation of functional tissues in 3-D hydrogels. Here, single-walled CNTs were incorporated into collagen hydrogels to fabricate (CNT/Col) hydrogels, which improved mechanical and electrical properties. The incorporation of CNTs (up to 1 wt%) exhibited no toxicity to cardiomyocytes and enhanced cell adhesion and elongation. Through the use of immunohistochemical staining, transmission electron microscopy, and intracellular calcium-transient measurement, the incorporation of CNTs was found to improve cell alignment and assembly remarkably, which led to the formation of engineered cardiac tissues with stronger contraction potential. Importantly, cardiac tissues based on CNT/Col hydrogels were noted to have better functionality. Collectively, the incorporation of CNTs into the Col hydrogels improved cell alignment and the performance of cardiac constructs. Our study suggests that CNT/Col hydrogels offer a promising tissue scaffold for cardiac constructs, and might serve as injectable biomaterials to deliver cell or drug molecules for cardiac regeneration following myocardial infarction in the near future. Keywords: carbon nanotubes, collagen hydrogel, cardiac constructs, cell alignment, tissue functionalitySun HYZhou JHuang ZQu LLLin NLiang CXDai RWTang LJTian FZDove Medical Pressarticlecarbon nanotubescollagen hydrogelcardiac constructscell alignmenttissue functionalityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 3109-3120 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	carbon nanotubes collagen hydrogel cardiac constructs cell alignment tissue functionality Medicine (General) R5-920
spellingShingle	carbon nanotubes collagen hydrogel cardiac constructs cell alignment tissue functionality Medicine (General) R5-920 Sun HY Zhou J Huang Z Qu LL Lin N Liang CX Dai RW Tang LJ Tian FZ Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
description	Hongyu Sun,* Jing Zhou,* Zhu Huang,* Linlin Qu,* Ning Lin,* Chengxiao Liang, Ruiwu Dai, Lijun Tang, Fuzhou Tian General Surgery Center, Chengdu Military General Hospital, Chengdu, China *These authors contributed equally to this work Abstract: Carbon nanotubes (CNTs) provide an essential 2-D microenvironment for cardiomyocyte growth and function. However, it remains to be elucidated whether CNT nanostructures can promote cell–cell integrity and facilitate the formation of functional tissues in 3-D hydrogels. Here, single-walled CNTs were incorporated into collagen hydrogels to fabricate (CNT/Col) hydrogels, which improved mechanical and electrical properties. The incorporation of CNTs (up to 1 wt%) exhibited no toxicity to cardiomyocytes and enhanced cell adhesion and elongation. Through the use of immunohistochemical staining, transmission electron microscopy, and intracellular calcium-transient measurement, the incorporation of CNTs was found to improve cell alignment and assembly remarkably, which led to the formation of engineered cardiac tissues with stronger contraction potential. Importantly, cardiac tissues based on CNT/Col hydrogels were noted to have better functionality. Collectively, the incorporation of CNTs into the Col hydrogels improved cell alignment and the performance of cardiac constructs. Our study suggests that CNT/Col hydrogels offer a promising tissue scaffold for cardiac constructs, and might serve as injectable biomaterials to deliver cell or drug molecules for cardiac regeneration following myocardial infarction in the near future. Keywords: carbon nanotubes, collagen hydrogel, cardiac constructs, cell alignment, tissue functionality
format	article
author	Sun HY Zhou J Huang Z Qu LL Lin N Liang CX Dai RW Tang LJ Tian FZ
author_facet	Sun HY Zhou J Huang Z Qu LL Lin N Liang CX Dai RW Tang LJ Tian FZ
author_sort	Sun HY
title	Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
title_short	Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
title_full	Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
title_fullStr	Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
title_full_unstemmed	Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
title_sort	carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/847e9403607e4cb191ecf3a42ae55b7e
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Carbon nanotube-incorporated collagen hydrogels improve cell alignment and the performance of cardiac constructs

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