Molecular Karyotyping on Populus simonii × P. nigra and the Derived Doubled Haploid

The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid Populus simonii ×...

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Autores principales:	Bo Liu, Sui Wang, Xiaoyan Tao, Caixia Liu, Guanzheng Qu, Quanwen Dou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Populus</i> <i>Populus simonii</i> × <i>P. nigra</i> doubled haploid repetitive sequence karyotype FISH Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/847e9914a2f7441eb682564792128f87
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spelling	oai:doaj.org-article:847e9914a2f7441eb682564792128f872021-11-11T16:53:43ZMolecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid10.3390/ijms2221114241422-00671661-6596https://doaj.org/article/847e9914a2f7441eb682564792128f872021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11424https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid <i>Populus simonii × P. nigra</i> (<i>P. xiaohei</i>) and the derived doubled haploids was carried out with labeled telomeres, rDNA, and two newly repetitive sequences as probes by fluorescence in situ hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with high-sequence homology were used, and the results showed that they presented the colocalized distribution signal in chromosomes. For <i>P. xiaohei</i>, pPD284_XHY produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six pairs of chromosomes, and the three pairs of chromosomes showed a significant difference in the hybridization between homologous chromosomes. The repeat probes used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY generated an additional hybridization site in the telomere region of chromosome 14. Moreover, two pairs of chromosomes showed differential hybridization distributions between homologous chromosomes. Comparisons of the distinguished chromosomes between hybrid and DH poplar showed that three pairs of chromosomes in the DH presented hybridization patterns that varied from those of the hybrid. The No. 8 chromosome in DH and one of the homologous chromosomes in <i>P. xiaohei</i> shared highly similar FISH patterns, which suggested the possibility of intact or mostly partial transfer of the chromosome between the hybrid and DH. Our study will contribute to understanding the genetic mechanism of chromosomal variation in <i>P. xiaohei</i> and derived DH plants.Bo LiuSui WangXiaoyan TaoCaixia LiuGuanzheng QuQuanwen DouMDPI AGarticle<i>Populus</i><i>Populus simonii</i> × <i>P. nigra</i>doubled haploidrepetitive sequencekaryotypeFISHBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11424, p 11424 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Populus</i> <i>Populus simonii</i> × <i>P. nigra</i> doubled haploid repetitive sequence karyotype FISH Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Populus</i> <i>Populus simonii</i> × <i>P. nigra</i> doubled haploid repetitive sequence karyotype FISH Biology (General) QH301-705.5 Chemistry QD1-999 Bo Liu Sui Wang Xiaoyan Tao Caixia Liu Guanzheng Qu Quanwen Dou Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
description	The molecular karyotype could represent the basic genetic make-up in a cell nucleus of an organism or species. A doubled haploid (DH) is a genotype formed from the chromosome doubling of haploid cells. In the present study, molecular karyotype analysis of the poplar hybrid <i>Populus simonii × P. nigra</i> (<i>P. xiaohei</i>) and the derived doubled haploids was carried out with labeled telomeres, rDNA, and two newly repetitive sequences as probes by fluorescence in situ hybridization (FISH). The tandem repeats, pPC349_XHY and pPD284_XHY, with high-sequence homology were used, and the results showed that they presented the colocalized distribution signal in chromosomes. For <i>P. xiaohei</i>, pPD284_XHY produced hybridizations in chromosomes 1, 5, 8, and 9 in the hybrid. The combination of pPD284_XHY, 45S rDNA, and 5S rDNA distinctly distinguished six pairs of chromosomes, and the three pairs of chromosomes showed a significant difference in the hybridization between homologous chromosomes. The repeat probes used produced similar FISH hybridizations in the DH; nevertheless, pPD284_XHY generated an additional hybridization site in the telomere region of chromosome 14. Moreover, two pairs of chromosomes showed differential hybridization distributions between homologous chromosomes. Comparisons of the distinguished chromosomes between hybrid and DH poplar showed that three pairs of chromosomes in the DH presented hybridization patterns that varied from those of the hybrid. The No. 8 chromosome in DH and one of the homologous chromosomes in <i>P. xiaohei</i> shared highly similar FISH patterns, which suggested the possibility of intact or mostly partial transfer of the chromosome between the hybrid and DH. Our study will contribute to understanding the genetic mechanism of chromosomal variation in <i>P. xiaohei</i> and derived DH plants.
format	article
author	Bo Liu Sui Wang Xiaoyan Tao Caixia Liu Guanzheng Qu Quanwen Dou
author_facet	Bo Liu Sui Wang Xiaoyan Tao Caixia Liu Guanzheng Qu Quanwen Dou
author_sort	Bo Liu
title	Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
title_short	Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
title_full	Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
title_fullStr	Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
title_full_unstemmed	Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid
title_sort	molecular karyotyping on <i>populus simonii</i> × <i>p. nigra</i> and the derived doubled haploid
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/847e9914a2f7441eb682564792128f87
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Molecular Karyotyping on <i>Populus simonii</i> × <i>P. nigra</i> and the Derived Doubled Haploid

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