RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates

RF1 attenuation enables efficient non-natural amino acid incorporation for production of homogeneous antibody drug conjugates

Abstract Amber codon suppression for the insertion of non-natural amino acids (nnAAs) is limited by competition with release factor 1 (RF1). Here we describe the genome engineering of a RF1 mutant strain that enhances suppression efficiency during cell-free protein synthesis, without significantly i...

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Autores principales: Gang Yin, Heather T. Stephenson, Junhao Yang, Xiaofan Li, Stephanie M. Armstrong, Tyler H. Heibeck, Cuong Tran, Mary Rose Masikat, Sihong Zhou, Ryan L. Stafford, Alice Y. Yam, John Lee, Alexander R. Steiner, Avinash Gill, Kalyani Penta, Sonia Pollitt, Ramesh Baliga, Christopher J. Murray, Christopher D. Thanos, Leslie M. McEvoy, Aaron K. Sato, Trevor J. Hallam
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/847eaf6ce18d47c0aa91ced7f6902fb0
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