Differential expression of transposable elements in the medaka melanoma model.

Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Frederik Helmprobst, Susanne Kneitz, Barbara Klotz, Magali Naville, Corentin Dechaud, Jean-Nicolas Volff, Manfred Schartl
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84879b231800422dad66f0d25963e9aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:84879b231800422dad66f0d25963e9aa
record_format	dspace
spelling	oai:doaj.org-article:84879b231800422dad66f0d25963e9aa2021-12-02T20:13:28ZDifferential expression of transposable elements in the medaka melanoma model.1932-620310.1371/journal.pone.0251713https://doaj.org/article/84879b231800422dad66f0d25963e9aa2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0251713https://doaj.org/toc/1932-6203Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.Frederik HelmprobstSusanne KneitzBarbara KlotzMagali NavilleCorentin DechaudJean-Nicolas VolffManfred SchartlPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0251713 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl Differential expression of transposable elements in the medaka melanoma model.
description	Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.
format	article
author	Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl
author_facet	Frederik Helmprobst Susanne Kneitz Barbara Klotz Magali Naville Corentin Dechaud Jean-Nicolas Volff Manfred Schartl
author_sort	Frederik Helmprobst
title	Differential expression of transposable elements in the medaka melanoma model.
title_short	Differential expression of transposable elements in the medaka melanoma model.
title_full	Differential expression of transposable elements in the medaka melanoma model.
title_fullStr	Differential expression of transposable elements in the medaka melanoma model.
title_full_unstemmed	Differential expression of transposable elements in the medaka melanoma model.
title_sort	differential expression of transposable elements in the medaka melanoma model.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/84879b231800422dad66f0d25963e9aa
work_keys_str_mv	AT frederikhelmprobst differentialexpressionoftransposableelementsinthemedakamelanomamodel AT susannekneitz differentialexpressionoftransposableelementsinthemedakamelanomamodel AT barbaraklotz differentialexpressionoftransposableelementsinthemedakamelanomamodel AT magalinaville differentialexpressionoftransposableelementsinthemedakamelanomamodel AT corentindechaud differentialexpressionoftransposableelementsinthemedakamelanomamodel AT jeannicolasvolff differentialexpressionoftransposableelementsinthemedakamelanomamodel AT manfredschartl differentialexpressionoftransposableelementsinthemedakamelanomamodel
_version_	1718374776907497472

Differential expression of transposable elements in the medaka melanoma model.

Ejemplares similares