Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells

Abstract In response to UV irradiation, translesion DNA synthesis (TLS) utilizes specialized DNA polymerases to bypass replication-blocking lesions. In a well-established polymerase switch model, Polη is thought to be a preferred TLS polymerase to insert correct nucleotides across from the thymine d...

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Autores principales: Tonghui Bi, Xiaohong Niu, Chunping Qin, Wei Xiao
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/84893909b2c14fa98bd3f2f0eb8fa9cd
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spelling oai:doaj.org-article:84893909b2c14fa98bd3f2f0eb8fa9cd2021-11-08T10:55:17ZGenetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells10.1038/s41598-021-00878-32045-2322https://doaj.org/article/84893909b2c14fa98bd3f2f0eb8fa9cd2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00878-3https://doaj.org/toc/2045-2322Abstract In response to UV irradiation, translesion DNA synthesis (TLS) utilizes specialized DNA polymerases to bypass replication-blocking lesions. In a well-established polymerase switch model, Polη is thought to be a preferred TLS polymerase to insert correct nucleotides across from the thymine dimer, and Rev1 plays a scaffold role through physical interaction with Polη and the Rev7 subunit of Polζ for continual DNA synthesis. Defective Polη causes a variant form of xeroderma pigmentosum (XPV), a disease with predisposition to sunlight-induced skin cancer. Previous studies revealed that expression of Rev1 alone is sufficient to confer enhanced UV damage tolerance in mammalian cells, which depends on its physical interaction with Polζ but is independent of Polη, a conclusion that appears to contradict current literature on the critical roles of Polη in TLS. To test a hypothesis that the Rev1 catalytic activity is required to backup Polη in TLS, we found that the Rev1 polymerase-dead mutation is synergistic with either Polη mutation or the Polη-interaction mutation in response to UV-induced DNA damage. On the other hand, functional complementation of polH cells by Polη relies on its physical interaction with Rev1. Hence, our studies reveal critical interactions between Rev1 and Polη in response to UV damage.Tonghui BiXiaohong NiuChunping QinWei XiaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Tonghui Bi
Xiaohong Niu
Chunping Qin
Wei Xiao
Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
description Abstract In response to UV irradiation, translesion DNA synthesis (TLS) utilizes specialized DNA polymerases to bypass replication-blocking lesions. In a well-established polymerase switch model, Polη is thought to be a preferred TLS polymerase to insert correct nucleotides across from the thymine dimer, and Rev1 plays a scaffold role through physical interaction with Polη and the Rev7 subunit of Polζ for continual DNA synthesis. Defective Polη causes a variant form of xeroderma pigmentosum (XPV), a disease with predisposition to sunlight-induced skin cancer. Previous studies revealed that expression of Rev1 alone is sufficient to confer enhanced UV damage tolerance in mammalian cells, which depends on its physical interaction with Polζ but is independent of Polη, a conclusion that appears to contradict current literature on the critical roles of Polη in TLS. To test a hypothesis that the Rev1 catalytic activity is required to backup Polη in TLS, we found that the Rev1 polymerase-dead mutation is synergistic with either Polη mutation or the Polη-interaction mutation in response to UV-induced DNA damage. On the other hand, functional complementation of polH cells by Polη relies on its physical interaction with Rev1. Hence, our studies reveal critical interactions between Rev1 and Polη in response to UV damage.
format article
author Tonghui Bi
Xiaohong Niu
Chunping Qin
Wei Xiao
author_facet Tonghui Bi
Xiaohong Niu
Chunping Qin
Wei Xiao
author_sort Tonghui Bi
title Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
title_short Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
title_full Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
title_fullStr Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
title_full_unstemmed Genetic and physical interactions between Polη and Rev1 in response to UV-induced DNA damage in mammalian cells
title_sort genetic and physical interactions between polη and rev1 in response to uv-induced dna damage in mammalian cells
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/84893909b2c14fa98bd3f2f0eb8fa9cd
work_keys_str_mv AT tonghuibi geneticandphysicalinteractionsbetweenpolēandrev1inresponsetouvinduceddnadamageinmammaliancells
AT xiaohongniu geneticandphysicalinteractionsbetweenpolēandrev1inresponsetouvinduceddnadamageinmammaliancells
AT chunpingqin geneticandphysicalinteractionsbetweenpolēandrev1inresponsetouvinduceddnadamageinmammaliancells
AT weixiao geneticandphysicalinteractionsbetweenpolēandrev1inresponsetouvinduceddnadamageinmammaliancells
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