The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture

BACKGROUND AND OBJECTIVE: Clomiphene citrate is a drug which used for superovulation in infertile women. Despite increasing oocyte number, its pregnancy rate is low. Endometrial stromal cells have pivotal role in implantation events. The aim of present investigation was to determine the effect of di...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M Khazaei, F Chobsaz, S . Khazaei
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2010
Materias:	human endometrium stromal cell cell culture clomiphene citrate Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/84920b2af6734230a695d9c4aaed7fb1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:84920b2af6734230a695d9c4aaed7fb1
record_format	dspace
spelling	oai:doaj.org-article:84920b2af6734230a695d9c4aaed7fb12021-11-10T09:00:22ZThe Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture1561-41072251-7170https://doaj.org/article/84920b2af6734230a695d9c4aaed7fb12010-06-01T00:00:00Zhttp://jbums.org/article-1-3466-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Clomiphene citrate is a drug which used for superovulation in infertile women. Despite increasing oocyte number, its pregnancy rate is low. Endometrial stromal cells have pivotal role in implantation events. The aim of present investigation was to determine the effect of different clomiphene citrate doses on morphology and proliferation of human endometrial stromal cell in in-vitro culture. METHODS: In this experimental study, human endometrial biopsies (n=7) chopped in sterile condition and digested by enzymatic methods (collagenase). Stromal cell harvested after using cell strainer and centrifugation. Their viability and cell number were determined with Trypan blue staining. Stromal cells divided into four groups: control and cases: (1, 5 and 10 µM clomiphene). Control group received DMEM/F12 media supplemental with FBS (5%) and antibiotic and cases groups received same media with one dose of clomiphene citrate. Duration of study was two weeks and cells were photographed at beginning and the end of study. Finally cell collected enzymaticly by tripsin (0.25%) and their viability and cell number were determined. FINDINGS: Morphological changes resembled in control and 1 µM groups and consist of oval cell transformation into large polyclonal fibroblast. 5 and 10µM doses of clomiphene citrate inhibit normal proliferation and morphological changes. There are significant differences between 5 and 10µM with control and 1µM groups (p<0.001).CONCLUSION: Clomiphene citrate showed dose dependant effect on endometrial stromal cell and higher doses inhibit proliferation and morphological changes of these cells.M Khazaei,F ChobsazS . KhazaeiBabol University of Medical Sciencesarticlehuman endometriumstromal cellcell cultureclomiphene citrateMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 12, Iss 2, Pp 7-12 (2010)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	human endometrium stromal cell cell culture clomiphene citrate Medicine R Medicine (General) R5-920
spellingShingle	human endometrium stromal cell cell culture clomiphene citrate Medicine R Medicine (General) R5-920 M Khazaei, F Chobsaz S . Khazaei The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
description	BACKGROUND AND OBJECTIVE: Clomiphene citrate is a drug which used for superovulation in infertile women. Despite increasing oocyte number, its pregnancy rate is low. Endometrial stromal cells have pivotal role in implantation events. The aim of present investigation was to determine the effect of different clomiphene citrate doses on morphology and proliferation of human endometrial stromal cell in in-vitro culture. METHODS: In this experimental study, human endometrial biopsies (n=7) chopped in sterile condition and digested by enzymatic methods (collagenase). Stromal cell harvested after using cell strainer and centrifugation. Their viability and cell number were determined with Trypan blue staining. Stromal cells divided into four groups: control and cases: (1, 5 and 10 µM clomiphene). Control group received DMEM/F12 media supplemental with FBS (5%) and antibiotic and cases groups received same media with one dose of clomiphene citrate. Duration of study was two weeks and cells were photographed at beginning and the end of study. Finally cell collected enzymaticly by tripsin (0.25%) and their viability and cell number were determined. FINDINGS: Morphological changes resembled in control and 1 µM groups and consist of oval cell transformation into large polyclonal fibroblast. 5 and 10µM doses of clomiphene citrate inhibit normal proliferation and morphological changes. There are significant differences between 5 and 10µM with control and 1µM groups (p<0.001).CONCLUSION: Clomiphene citrate showed dose dependant effect on endometrial stromal cell and higher doses inhibit proliferation and morphological changes of these cells.
format	article
author	M Khazaei, F Chobsaz S . Khazaei
author_facet	M Khazaei, F Chobsaz S . Khazaei
author_sort	M Khazaei,
title	The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
title_short	The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
title_full	The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
title_fullStr	The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
title_full_unstemmed	The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture
title_sort	effect of different doses of clomiphen citrate on morphology and proliferation of human endometrial stromal cells in in-vitro culture
publisher	Babol University of Medical Sciences
publishDate	2010
url	https://doaj.org/article/84920b2af6734230a695d9c4aaed7fb1
work_keys_str_mv	AT mkhazaei theeffectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture AT fchobsaz theeffectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture AT skhazaei theeffectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture AT mkhazaei effectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture AT fchobsaz effectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture AT skhazaei effectofdifferentdosesofclomiphencitrateonmorphologyandproliferationofhumanendometrialstromalcellsininvitroculture
_version_	1718440301984481280

The Effect of Different Doses of Clomiphen Citrate on Morphology and Proliferation of Human Endometrial Stromal Cells in In-Vitro Culture

Ejemplares similares