Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles

Endothelialization by materials provides a promising approach for the rapid re-endothelialization of a cardiovascular implantation. Although previous studies have focused on improving endothelialization through the immobilization of bioactive molecules onto the surface of biodegradable implants, com...

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Autores principales:	Jiwon Woo, Kyoung-Won Ko, Seung-Gyu Cha, Yun Heo, Dong Keun Han
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biodegradable implant surface modification fibronectin polyethylenimine polydopamine immobilization of EVs Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/8494b2a25a30467f888f4b5e45e2a35c
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spelling	oai:doaj.org-article:8494b2a25a30467f888f4b5e45e2a35c2021-11-11T18:42:27ZComparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles10.3390/polym132136432073-4360https://doaj.org/article/8494b2a25a30467f888f4b5e45e2a35c2021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3643https://doaj.org/toc/2073-4360Endothelialization by materials provides a promising approach for the rapid re-endothelialization of a cardiovascular implantation. Although previous studies have focused on improving endothelialization through the immobilization of bioactive molecules onto the surface of biodegradable implants, comparative studies of effective surface modification have not yet been reported. Here, we conducted a comparative study on the surface modification of poly(lactide-co-glycolide) (PLGA)-based composites to graft mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) using three different materials, fibronectin (FN), polyethylenimine (PEI), and polydopamine (PDA), which have different bond strengths of ligand–receptor interaction, ionic bond, and covalent bond, respectively. Further in vitro analysis exhibited that MSC-EVs released from all modified films sustainably, but the MSC-EVs grafted onto the surface coated with PEI are more effective than other groups in increasing angiogenesis and reducing the inflammatory responses in endothelial cells. Therefore, the overall results demonstrated that PEI is a desirable coating reagent for the immobilization of MSC-EVs on the surface of biodegradable implants.Jiwon WooKyoung-Won KoSeung-Gyu ChaYun HeoDong Keun HanMDPI AGarticlebiodegradable implantsurface modificationfibronectinpolyethyleniminepolydopamineimmobilization of EVsOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3643, p 3643 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biodegradable implant surface modification fibronectin polyethylenimine polydopamine immobilization of EVs Organic chemistry QD241-441
spellingShingle	biodegradable implant surface modification fibronectin polyethylenimine polydopamine immobilization of EVs Organic chemistry QD241-441 Jiwon Woo Kyoung-Won Ko Seung-Gyu Cha Yun Heo Dong Keun Han Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
description	Endothelialization by materials provides a promising approach for the rapid re-endothelialization of a cardiovascular implantation. Although previous studies have focused on improving endothelialization through the immobilization of bioactive molecules onto the surface of biodegradable implants, comparative studies of effective surface modification have not yet been reported. Here, we conducted a comparative study on the surface modification of poly(lactide-co-glycolide) (PLGA)-based composites to graft mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) using three different materials, fibronectin (FN), polyethylenimine (PEI), and polydopamine (PDA), which have different bond strengths of ligand–receptor interaction, ionic bond, and covalent bond, respectively. Further in vitro analysis exhibited that MSC-EVs released from all modified films sustainably, but the MSC-EVs grafted onto the surface coated with PEI are more effective than other groups in increasing angiogenesis and reducing the inflammatory responses in endothelial cells. Therefore, the overall results demonstrated that PEI is a desirable coating reagent for the immobilization of MSC-EVs on the surface of biodegradable implants.
format	article
author	Jiwon Woo Kyoung-Won Ko Seung-Gyu Cha Yun Heo Dong Keun Han
author_facet	Jiwon Woo Kyoung-Won Ko Seung-Gyu Cha Yun Heo Dong Keun Han
author_sort	Jiwon Woo
title	Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
title_short	Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
title_full	Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
title_fullStr	Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
title_full_unstemmed	Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles
title_sort	comparison of surface functionalization of plga composite to immobilize extracellular vesicles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/8494b2a25a30467f888f4b5e45e2a35c
work_keys_str_mv	AT jiwonwoo comparisonofsurfacefunctionalizationofplgacompositetoimmobilizeextracellularvesicles AT kyoungwonko comparisonofsurfacefunctionalizationofplgacompositetoimmobilizeextracellularvesicles AT seunggyucha comparisonofsurfacefunctionalizationofplgacompositetoimmobilizeextracellularvesicles AT yunheo comparisonofsurfacefunctionalizationofplgacompositetoimmobilizeextracellularvesicles AT dongkeunhan comparisonofsurfacefunctionalizationofplgacompositetoimmobilizeextracellularvesicles
_version_	1718431772455206912

Comparison of Surface Functionalization of PLGA Composite to Immobilize Extracellular Vesicles

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