Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation

Cloned animals generated by the somatic cell nuclear transfer (SCNT) approach are valuable for the farm animal industry and biomedical science. Nevertheless, the extremely low developmental efficiency of cloned embryos hinders the application of SCNT. Low developmental competence is related to the h...

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Autores principales:	Xiao Wu, Huaxing Zhao, Junkun Lai, Ning Zhang, Junsong Shi, Rong Zhou, Qiaoyun Su, Enqin Zheng, Zheng Xu, Sixiu Huang, Linjun Hong, Ting Gu, Jie Yang, Huaqiang Yang, Gengyuan Cai, Zhenfang Wu, Zicong Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SCNT porcine IL17D apoptosis EGA Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/849792be0cc94e56b6ddf7432418f692
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spelling	oai:doaj.org-article:849792be0cc94e56b6ddf7432418f6922021-11-25T16:14:20ZInterleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation10.3390/ani111130622076-2615https://doaj.org/article/849792be0cc94e56b6ddf7432418f6922021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3062https://doaj.org/toc/2076-2615Cloned animals generated by the somatic cell nuclear transfer (SCNT) approach are valuable for the farm animal industry and biomedical science. Nevertheless, the extremely low developmental efficiency of cloned embryos hinders the application of SCNT. Low developmental competence is related to the higher apoptosis level in cloned embryos than in fertilization-derived counterparts. Interleukin 17D (IL17D) expression is up-regulated during early mouse embryo development and is required for normal development of mouse embryos by inhibiting apoptosis. This study aimed to investigate whether IL17D plays roles in regulating pig SCNT embryo development. Supplementation of IL17D to culture medium improved the developmental competence and decreased the cell apoptosis level in cloned porcine embryos. The transcriptome data indicated that IL17D activated apoptosis-associated pathways and promoted global gene expression at embryonic genome activation (EGA) stage in treated pig SCNT embryos. Treating pig SCNT embryos with IL17D up-regulated expression of <i>GADD45B</i>, which is functional in inhibiting apoptosis and promoting EGA. Overexpression of <i>GADD45B</i> enhanced the developmental efficiency of cloned pig embryos. These results suggested that IL17D treatment enhanced the developmental ability of cloned pig embryos by suppressing apoptosis and promoting EGA, which was related to the up-regulation of <i>GADD45B</i> expression. This study demonstrated the roles of IL17D in early development of porcine SCNT embryos and provided a new approach to improve the developmental efficiency of cloned porcine embryos.Xiao WuHuaxing ZhaoJunkun LaiNing ZhangJunsong ShiRong ZhouQiaoyun SuEnqin ZhengZheng XuSixiu HuangLinjun HongTing GuJie YangHuaqiang YangGengyuan CaiZhenfang WuZicong LiMDPI AGarticleSCNTporcineIL17DapoptosisEGAVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3062, p 3062 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SCNT porcine IL17D apoptosis EGA Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	SCNT porcine IL17D apoptosis EGA Veterinary medicine SF600-1100 Zoology QL1-991 Xiao Wu Huaxing Zhao Junkun Lai Ning Zhang Junsong Shi Rong Zhou Qiaoyun Su Enqin Zheng Zheng Xu Sixiu Huang Linjun Hong Ting Gu Jie Yang Huaqiang Yang Gengyuan Cai Zhenfang Wu Zicong Li Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
description	Cloned animals generated by the somatic cell nuclear transfer (SCNT) approach are valuable for the farm animal industry and biomedical science. Nevertheless, the extremely low developmental efficiency of cloned embryos hinders the application of SCNT. Low developmental competence is related to the higher apoptosis level in cloned embryos than in fertilization-derived counterparts. Interleukin 17D (IL17D) expression is up-regulated during early mouse embryo development and is required for normal development of mouse embryos by inhibiting apoptosis. This study aimed to investigate whether IL17D plays roles in regulating pig SCNT embryo development. Supplementation of IL17D to culture medium improved the developmental competence and decreased the cell apoptosis level in cloned porcine embryos. The transcriptome data indicated that IL17D activated apoptosis-associated pathways and promoted global gene expression at embryonic genome activation (EGA) stage in treated pig SCNT embryos. Treating pig SCNT embryos with IL17D up-regulated expression of <i>GADD45B</i>, which is functional in inhibiting apoptosis and promoting EGA. Overexpression of <i>GADD45B</i> enhanced the developmental efficiency of cloned pig embryos. These results suggested that IL17D treatment enhanced the developmental ability of cloned pig embryos by suppressing apoptosis and promoting EGA, which was related to the up-regulation of <i>GADD45B</i> expression. This study demonstrated the roles of IL17D in early development of porcine SCNT embryos and provided a new approach to improve the developmental efficiency of cloned porcine embryos.
format	article
author	Xiao Wu Huaxing Zhao Junkun Lai Ning Zhang Junsong Shi Rong Zhou Qiaoyun Su Enqin Zheng Zheng Xu Sixiu Huang Linjun Hong Ting Gu Jie Yang Huaqiang Yang Gengyuan Cai Zhenfang Wu Zicong Li
author_facet	Xiao Wu Huaxing Zhao Junkun Lai Ning Zhang Junsong Shi Rong Zhou Qiaoyun Su Enqin Zheng Zheng Xu Sixiu Huang Linjun Hong Ting Gu Jie Yang Huaqiang Yang Gengyuan Cai Zhenfang Wu Zicong Li
author_sort	Xiao Wu
title	Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
title_short	Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
title_full	Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
title_fullStr	Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
title_full_unstemmed	Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation
title_sort	interleukin 17d enhances the developmental competence of cloned pig embryos by inhibiting apoptosis and promoting embryonic genome activation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/849792be0cc94e56b6ddf7432418f692
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Interleukin 17D Enhances the Developmental Competence of Cloned Pig Embryos by Inhibiting Apoptosis and Promoting Embryonic Genome Activation

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