Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Abstract New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Binh Thanh Vu, Sophia Allaf Shahin, Jonas Croissant, Yevhen Fatieiev, Kotaro Matsumoto, Tan Le-Hoang Doan, Tammy Yik, Shirleen Simargi, Altagracia Conteras, Laura Ratliff, Chiara Mauriello Jimenez, Laurence Raehm, Niveen Khashab, Jean-Olivier Durand, Carlotta Glackin, Fuyuhiko Tamanoi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/849a54837dce44fab69e9ca907705965
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:849a54837dce44fab69e9ca907705965
record_format	dspace
spelling	oai:doaj.org-article:849a54837dce44fab69e9ca9077059652021-12-02T15:08:00ZChick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer10.1038/s41598-018-25573-82045-2322https://doaj.org/article/849a54837dce44fab69e9ca9077059652018-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25573-8https://doaj.org/toc/2045-2322Abstract New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.Binh Thanh VuSophia Allaf ShahinJonas CroissantYevhen FatieievKotaro MatsumotoTan Le-Hoang DoanTammy YikShirleen SimargiAltagracia ConterasLaura RatliffChiara Mauriello JimenezLaurence RaehmNiveen KhashabJean-Olivier DurandCarlotta GlackinFuyuhiko TamanoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Binh Thanh Vu Sophia Allaf Shahin Jonas Croissant Yevhen Fatieiev Kotaro Matsumoto Tan Le-Hoang Doan Tammy Yik Shirleen Simargi Altagracia Conteras Laura Ratliff Chiara Mauriello Jimenez Laurence Raehm Niveen Khashab Jean-Olivier Durand Carlotta Glackin Fuyuhiko Tamanoi Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
description	Abstract New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.
format	article
author	Binh Thanh Vu Sophia Allaf Shahin Jonas Croissant Yevhen Fatieiev Kotaro Matsumoto Tan Le-Hoang Doan Tammy Yik Shirleen Simargi Altagracia Conteras Laura Ratliff Chiara Mauriello Jimenez Laurence Raehm Niveen Khashab Jean-Olivier Durand Carlotta Glackin Fuyuhiko Tamanoi
author_facet	Binh Thanh Vu Sophia Allaf Shahin Jonas Croissant Yevhen Fatieiev Kotaro Matsumoto Tan Le-Hoang Doan Tammy Yik Shirleen Simargi Altagracia Conteras Laura Ratliff Chiara Mauriello Jimenez Laurence Raehm Niveen Khashab Jean-Olivier Durand Carlotta Glackin Fuyuhiko Tamanoi
author_sort	Binh Thanh Vu
title	Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
title_short	Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
title_full	Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
title_fullStr	Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
title_full_unstemmed	Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
title_sort	chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/849a54837dce44fab69e9ca907705965
work_keys_str_mv	AT binhthanhvu chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT sophiaallafshahin chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT jonascroissant chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT yevhenfatieiev chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT kotaromatsumoto chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT tanlehoangdoan chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT tammyyik chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT shirleensimargi chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT altagraciaconteras chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT lauraratliff chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT chiaramauriellojimenez chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT laurenceraehm chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT niveenkhashab chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT jeanolivierdurand chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT carlottaglackin chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer AT fuyuhikotamanoi chickchorioallantoicmembraneassayasaninvivomodeltostudytheeffectofnanoparticlebasedanticancerdrugsinovariancancer
_version_	1718388327105691648

Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Ejemplares similares