A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering

A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering

Fluorescent RNA aptamers could potentially be used as protein-independent reporters of transgene expression in plants. Here, the authors report that an optimized RNA aptamer, developed from Broccoli, can be used to detect transgene expression in stable and transiently transformed plant tissue.

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Autores principales: Jiuyuan Bai, Yao Luo, Xin Wang, Shi Li, Mei Luo, Meng Yin, Yuanli Zuo, Guolin Li, Junyu Yao, Hua Yang, Mingdi Zhang, Wei Wei, Maolin Wang, Rui Wang, Chunhai Fan, Yun Zhao
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/84a0865d01c248c898d6bd212315cc16
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spelling oai:doaj.org-article:84a0865d01c248c898d6bd212315cc162021-12-02T16:31:49ZA protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering10.1038/s41467-020-17497-72041-1723https://doaj.org/article/84a0865d01c248c898d6bd212315cc162020-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17497-7https://doaj.org/toc/2041-1723Fluorescent RNA aptamers could potentially be used as protein-independent reporters of transgene expression in plants. Here, the authors report that an optimized RNA aptamer, developed from Broccoli, can be used to detect transgene expression in stable and transiently transformed plant tissue.Jiuyuan BaiYao LuoXin WangShi LiMei LuoMeng YinYuanli ZuoGuolin LiJunyu YaoHua YangMingdi ZhangWei WeiMaolin WangRui WangChunhai FanYun ZhaoNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jiuyuan Bai
Yao Luo
Xin Wang
Shi Li
Mei Luo
Meng Yin
Yuanli Zuo
Guolin Li
Junyu Yao
Hua Yang
Mingdi Zhang
Wei Wei
Maolin Wang
Rui Wang
Chunhai Fan
Yun Zhao
A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
description Fluorescent RNA aptamers could potentially be used as protein-independent reporters of transgene expression in plants. Here, the authors report that an optimized RNA aptamer, developed from Broccoli, can be used to detect transgene expression in stable and transiently transformed plant tissue.
format article
author Jiuyuan Bai
Yao Luo
Xin Wang
Shi Li
Mei Luo
Meng Yin
Yuanli Zuo
Guolin Li
Junyu Yao
Hua Yang
Mingdi Zhang
Wei Wei
Maolin Wang
Rui Wang
Chunhai Fan
Yun Zhao
author_facet Jiuyuan Bai
Yao Luo
Xin Wang
Shi Li
Mei Luo
Meng Yin
Yuanli Zuo
Guolin Li
Junyu Yao
Hua Yang
Mingdi Zhang
Wei Wei
Maolin Wang
Rui Wang
Chunhai Fan
Yun Zhao
author_sort Jiuyuan Bai
title A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
title_short A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
title_full A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
title_fullStr A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
title_full_unstemmed A protein-independent fluorescent RNA aptamer reporter system for plant genetic engineering
title_sort protein-independent fluorescent rna aptamer reporter system for plant genetic engineering
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/84a0865d01c248c898d6bd212315cc16
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