Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin

Background and purpose: Doxorubicin (DOX) as a chemotherapeutic agent has been widely used in the treatment of various types of cancer. However, DOX exerts a toxic effect on normal tissues such as the brain. Furanocoumarins reduce the risk of cardiovascular and brain diseases because of their antiox...

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Autores principales:	Fereshteh Jalilian, Maryam Moieni-Arya, Leila Hosseinzadeh, Yalda Shokoohinia
Formato:	article
Lenguaje:	EN
Publicado:	Wolters Kluwer Medknow Publications 2022
Materias:	apoptosis; cytotoxicity; doxorubicin; isoimperatorin; oxypeucedanin; prangos ferulacea. Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/84a1c8348c5b478ab8411333ecd6d04c
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spelling	oai:doaj.org-article:84a1c8348c5b478ab8411333ecd6d04c2021-11-19T12:17:03ZOxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin1735-53621735-941410.4103/1735-5362.329922https://doaj.org/article/84a1c8348c5b478ab8411333ecd6d04c2022-01-01T00:00:00Zhttp://www.rpsjournal.net/article.asp?issn=1735-5362;year=2022;volume=17;issue=1;spage=12;epage=21;aulast=Jalilianhttps://doaj.org/toc/1735-5362https://doaj.org/toc/1735-9414Background and purpose: Doxorubicin (DOX) as a chemotherapeutic agent has been widely used in the treatment of various types of cancer. However, DOX exerts a toxic effect on normal tissues such as the brain. Furanocoumarins reduce the risk of cardiovascular and brain diseases because of their antioxidant activities. This study has been designed, for the first time, to evaluate the effect of known furanocoumarins oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl on oxidative stress and apoptosis induced by DOX toward pheochromocytoma cell line (PC12). Experimental approach: NMR and MASS spectrometers were used to characterize the isolated compounds. The protective effects of isolated compounds on DOX-induced cytotoxicity in PC12 cells were examined by MTT assay. PC12 cells were pretreated with oxypeucedanin and isoimperatorin for 2 and 21 h, respectively, subsequently exposure to DOX at IC50 concentration. Then, mitochondrial membrane potential (MMP), Bax and Bcl2 mRNA expressions, caspase-3 activation, and the generation of intracellular reactive oxygen species (ROS) were measured after 24 h. Findings/Results: Pretreatment with oxypeucedanin and isoimperatorin significantly decreased DOX-induced apoptosis through reduction of caspase-3 activity and ROS generation and an increase in MMP. In addition, our finding showed pretreatment with these compounds leads to regulation of Bcl-2. Conclusion and implications: Taken together our observation indicated that oxypeucedanin and isoimperatorin have a protective effect against apoptosis induced by DOX in PC12 cells by inhibition of ROS production.Fereshteh JalilianMaryam Moieni-AryaLeila HosseinzadehYalda ShokoohiniaWolters Kluwer Medknow Publicationsarticleapoptosis; cytotoxicity; doxorubicin; isoimperatorin; oxypeucedanin; prangos ferulacea.Pharmacy and materia medicaRS1-441ENResearch in Pharmaceutical Sciences, Vol 17, Iss 1, Pp 12-21 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	apoptosis; cytotoxicity; doxorubicin; isoimperatorin; oxypeucedanin; prangos ferulacea. Pharmacy and materia medica RS1-441
spellingShingle	apoptosis; cytotoxicity; doxorubicin; isoimperatorin; oxypeucedanin; prangos ferulacea. Pharmacy and materia medica RS1-441 Fereshteh Jalilian Maryam Moieni-Arya Leila Hosseinzadeh Yalda Shokoohinia Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
description	Background and purpose: Doxorubicin (DOX) as a chemotherapeutic agent has been widely used in the treatment of various types of cancer. However, DOX exerts a toxic effect on normal tissues such as the brain. Furanocoumarins reduce the risk of cardiovascular and brain diseases because of their antioxidant activities. This study has been designed, for the first time, to evaluate the effect of known furanocoumarins oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl on oxidative stress and apoptosis induced by DOX toward pheochromocytoma cell line (PC12). Experimental approach: NMR and MASS spectrometers were used to characterize the isolated compounds. The protective effects of isolated compounds on DOX-induced cytotoxicity in PC12 cells were examined by MTT assay. PC12 cells were pretreated with oxypeucedanin and isoimperatorin for 2 and 21 h, respectively, subsequently exposure to DOX at IC50 concentration. Then, mitochondrial membrane potential (MMP), Bax and Bcl2 mRNA expressions, caspase-3 activation, and the generation of intracellular reactive oxygen species (ROS) were measured after 24 h. Findings/Results: Pretreatment with oxypeucedanin and isoimperatorin significantly decreased DOX-induced apoptosis through reduction of caspase-3 activity and ROS generation and an increase in MMP. In addition, our finding showed pretreatment with these compounds leads to regulation of Bcl-2. Conclusion and implications: Taken together our observation indicated that oxypeucedanin and isoimperatorin have a protective effect against apoptosis induced by DOX in PC12 cells by inhibition of ROS production.
format	article
author	Fereshteh Jalilian Maryam Moieni-Arya Leila Hosseinzadeh Yalda Shokoohinia
author_facet	Fereshteh Jalilian Maryam Moieni-Arya Leila Hosseinzadeh Yalda Shokoohinia
author_sort	Fereshteh Jalilian
title	Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
title_short	Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
title_full	Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
title_fullStr	Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
title_full_unstemmed	Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
title_sort	oxypeucedanin and isoimperatorin extracted from prangos ferulacea (l.) lindl protect pc12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin
publisher	Wolters Kluwer Medknow Publications
publishDate	2022
url	https://doaj.org/article/84a1c8348c5b478ab8411333ecd6d04c
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Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin

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