Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity
Marco Cantisani,1 Mariateresa Vitiello,2 Annarita Falanga,1 Emiliana Finamore,2 Marilena Galdiero,2 Stefania Galdiero11Department of Biological Sciences, CIRPeB and IBB CNR, University of Naples "Federico II," Napoli, Italy; 2Department of Experimental Medicine, II Universi...
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Dove Medical Press
2012
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oai:doaj.org-article:84a32f692817457f8702c78e112449da2021-12-02T02:29:50ZPeptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity1176-91141178-2013https://doaj.org/article/84a32f692817457f8702c78e112449da2012-05-01T00:00:00Zhttp://www.dovepress.com/peptides-complementary-to-the-active-loop-of-porin-p2-from-haemophilus-a9891https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Marco Cantisani,1 Mariateresa Vitiello,2 Annarita Falanga,1 Emiliana Finamore,2 Marilena Galdiero,2 Stefania Galdiero11Department of Biological Sciences, CIRPeB and IBB CNR, University of Naples "Federico II," Napoli, Italy; 2Department of Experimental Medicine, II University of Naples, Napoli, ItalyAbstract: Haemophilus influenzae type b (Hib) is one of the leading causes of invasive bacterial infection in young children. It is characterized by inflammation that is mainly mediated by cytokines and chemokines. One of the most abundant components of the Hib outer membrane is the P2 porin, which has been shown to induce the release of several inflammatory cytokines. A synthetic peptide corresponding to loop L7 of the porin activates JNK and p38 mitogen-activated protein kinase (MAPK) pathways. We report a novel use of the complementary peptide approach to design a peptide that is able to bind selectively to the protein P2, thereby reducing its activity. This work provides insights into essential molecular details of P2 that may affect the pathogenesis of Hib infections where interruption of the signaling cascade could represent an attractive therapeutic strategy.Keywords: complementary-peptide, rational design, porinGaldiero SGaldiero MFinamore EFalanga AVitiello MCantisani MDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 2361-2371 (2012) |
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DOAJ |
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Medicine (General) R5-920 |
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Medicine (General) R5-920 Galdiero S Galdiero M Finamore E Falanga A Vitiello M Cantisani M Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| description |
Marco Cantisani,1 Mariateresa Vitiello,2 Annarita Falanga,1 Emiliana Finamore,2 Marilena Galdiero,2 Stefania Galdiero11Department of Biological Sciences, CIRPeB and IBB CNR, University of Naples "Federico II," Napoli, Italy; 2Department of Experimental Medicine, II University of Naples, Napoli, ItalyAbstract: Haemophilus influenzae type b (Hib) is one of the leading causes of invasive bacterial infection in young children. It is characterized by inflammation that is mainly mediated by cytokines and chemokines. One of the most abundant components of the Hib outer membrane is the P2 porin, which has been shown to induce the release of several inflammatory cytokines. A synthetic peptide corresponding to loop L7 of the porin activates JNK and p38 mitogen-activated protein kinase (MAPK) pathways. We report a novel use of the complementary peptide approach to design a peptide that is able to bind selectively to the protein P2, thereby reducing its activity. This work provides insights into essential molecular details of P2 that may affect the pathogenesis of Hib infections where interruption of the signaling cascade could represent an attractive therapeutic strategy.Keywords: complementary-peptide, rational design, porin |
| format |
article |
| author |
Galdiero S Galdiero M Finamore E Falanga A Vitiello M Cantisani M |
| author_facet |
Galdiero S Galdiero M Finamore E Falanga A Vitiello M Cantisani M |
| author_sort |
Galdiero S |
| title |
Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| title_short |
Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| title_full |
Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| title_fullStr |
Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| title_full_unstemmed |
Peptides complementary to the active loop of porin P2 from Haemophilus influenzae modulate its activity |
| title_sort |
peptides complementary to the active loop of porin p2 from haemophilus influenzae modulate its activity |
| publisher |
Dove Medical Press |
| publishDate |
2012 |
| url |
https://doaj.org/article/84a32f692817457f8702c78e112449da |
| work_keys_str_mv |
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