Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.

GABAA receptor-associated protein (GABARAP) was initially identified as a protein that interacts with GABAA receptor. Although LC3 (microtubule-associated protein 1 light chain 3), a GABARAP homolog, has been localized in the dendrites and cell bodies of neurons under normal conditions, the subcellu...

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Autores principales:	Masato Koike, Isei Tanida, Tomohisa Nanao, Norihiro Tada, Jun-ichi Iwata, Takashi Ueno, Eiki Kominami, Yasuo Uchiyama
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84a39521f012497ab6c1d2ae47649910
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spelling	oai:doaj.org-article:84a39521f012497ab6c1d2ae476499102021-11-18T07:46:11ZEnrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.1932-620310.1371/journal.pone.0063568https://doaj.org/article/84a39521f012497ab6c1d2ae476499102013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23671684/?tool=EBIhttps://doaj.org/toc/1932-6203GABAA receptor-associated protein (GABARAP) was initially identified as a protein that interacts with GABAA receptor. Although LC3 (microtubule-associated protein 1 light chain 3), a GABARAP homolog, has been localized in the dendrites and cell bodies of neurons under normal conditions, the subcellular distribution of GABARAP in neurons remains unclear. Subcellular fractionation indicated that endogenous GABARAP was localized to the microsome-enriched and synaptic vesicle-enriched fractions of mouse brain as GABARAP-I, an unlipidated form. To investigate the distribution of GABARAP in neurons, we generated GFP-GABARAP transgenic mice. Immunohistochemistry in these transgenic mice showed that positive signals for GFP-GABARAP were widely distributed in neurons in various brain regions, including the hippocampus and cerebellum. Interestingly, intense diffuse and/or fibrillary expression of GFP-GABARAP was detected along the axonal initial segments (AIS) of hippocampal pyramidal neurons and cerebellar Purkinje cells, in addition to the cell bodies and dendrites of these neurons. In contrast, only slight amounts of LC3 were detected along the AIS of these neurons, while diffuse and/or fibrillary staining for LC3 was mainly detected in their cell bodies and dendrites. These results indicated that, compared with LC3, GABARAP is enriched in the AIS, in addition to the cell bodies and dendrites, of these hippocampal pyramidal neurons and cerebellar Purkinje cells.Masato KoikeIsei TanidaTomohisa NanaoNorihiro TadaJun-ichi IwataTakashi UenoEiki KominamiYasuo UchiyamaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 5, p e63568 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Masato Koike Isei Tanida Tomohisa Nanao Norihiro Tada Jun-ichi Iwata Takashi Ueno Eiki Kominami Yasuo Uchiyama Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
description	GABAA receptor-associated protein (GABARAP) was initially identified as a protein that interacts with GABAA receptor. Although LC3 (microtubule-associated protein 1 light chain 3), a GABARAP homolog, has been localized in the dendrites and cell bodies of neurons under normal conditions, the subcellular distribution of GABARAP in neurons remains unclear. Subcellular fractionation indicated that endogenous GABARAP was localized to the microsome-enriched and synaptic vesicle-enriched fractions of mouse brain as GABARAP-I, an unlipidated form. To investigate the distribution of GABARAP in neurons, we generated GFP-GABARAP transgenic mice. Immunohistochemistry in these transgenic mice showed that positive signals for GFP-GABARAP were widely distributed in neurons in various brain regions, including the hippocampus and cerebellum. Interestingly, intense diffuse and/or fibrillary expression of GFP-GABARAP was detected along the axonal initial segments (AIS) of hippocampal pyramidal neurons and cerebellar Purkinje cells, in addition to the cell bodies and dendrites of these neurons. In contrast, only slight amounts of LC3 were detected along the AIS of these neurons, while diffuse and/or fibrillary staining for LC3 was mainly detected in their cell bodies and dendrites. These results indicated that, compared with LC3, GABARAP is enriched in the AIS, in addition to the cell bodies and dendrites, of these hippocampal pyramidal neurons and cerebellar Purkinje cells.
format	article
author	Masato Koike Isei Tanida Tomohisa Nanao Norihiro Tada Jun-ichi Iwata Takashi Ueno Eiki Kominami Yasuo Uchiyama
author_facet	Masato Koike Isei Tanida Tomohisa Nanao Norihiro Tada Jun-ichi Iwata Takashi Ueno Eiki Kominami Yasuo Uchiyama
author_sort	Masato Koike
title	Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
title_short	Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
title_full	Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
title_fullStr	Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
title_full_unstemmed	Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.
title_sort	enrichment of gabarap relative to lc3 in the axonal initial segments of neurons.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/84a39521f012497ab6c1d2ae47649910
work_keys_str_mv	AT masatokoike enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT iseitanida enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT tomohisananao enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT norihirotada enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT junichiiwata enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT takashiueno enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT eikikominami enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons AT yasuouchiyama enrichmentofgabaraprelativetolc3intheaxonalinitialsegmentsofneurons
_version_	1718422984318779392

Enrichment of GABARAP relative to LC3 in the axonal initial segments of neurons.

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