Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.

<h4>Background</h4>The Arabidopsis genome contains 18 genes that are predicted to encode Ovate Family Proteins (AtOFPs), a protein family characterized by a conserved OVATE domain, an approximately 70-amino acid domain that was originally found in tomato OVATE protein. Among AtOFP family...

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Autores principales:	Shucai Wang, Ying Chang, Jianjun Guo, Qingning Zeng, Brian E Ellis, Jin-Gui Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84a850af97fd475a97309e5d8eafab8f
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spelling	oai:doaj.org-article:84a850af97fd475a97309e5d8eafab8f2021-11-18T06:47:27ZArabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.1932-620310.1371/journal.pone.0023896https://doaj.org/article/84a850af97fd475a97309e5d8eafab8f2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21886836/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The Arabidopsis genome contains 18 genes that are predicted to encode Ovate Family Proteins (AtOFPs), a protein family characterized by a conserved OVATE domain, an approximately 70-amino acid domain that was originally found in tomato OVATE protein. Among AtOFP family members, AtOFP1 has been shown to suppress cell elongation, in part, by suppressing the expression of AtGA20ox1, AtOFP4 has been shown to regulate secondary cell wall formation by interact with KNOTTED1-LIKE HOMEODOMAIN PROTEIN 7 (KNAT7), and AtOFP5 has been shown to regulate the activity of a BEL1-LIKEHOMEODOMAIN 1(BLH1)-KNAT3 complex during early embryo sac development, but little is known about the function of other AtOFPs.<h4>Methodology/principal findings</h4>We demonstrated here that AtOFP proteins could function as effective transcriptional repressors in the Arabidopsis protoplast transient expression system. The analysis of loss-of-function alleles of AtOFPs suggested AtOFP genes may have overlapping function in regulating plant growth and development, because none of the single mutants identified, including T-DNA insertion mutants in AtOFP1, AtOFP4, AtOFP8, AtOFP10, AtOFP15 and AtOFP16, displayed any apparent morphological defects. Further, Atofp1 Atofp4 and Atofp15 Atofp16 double mutants still did not differ significantly from wild-type. On the other hand, plants overexpressing AtOFP genes displayed a number of abnormal phenotypes, which could be categorized into three distinct classes, suggesting that AtOFP genes may also have diverse functions in regulating plant growth and development. Further analysis suggested that AtOFP1 regulates cotyledon development in a postembryonic manner, and global transcript profiling revealed that it suppress the expression of many other genes.<h4>Conclusions/significance</h4>Our results showed that AtOFPs function as transcriptional repressors and they regulate multiple aspects of plant growth and development. These results provided the first overview of a previously unknown transcriptional repressor family, and revealed their possible roles in plant growth and development.Shucai WangYing ChangJianjun GuoQingning ZengBrian E EllisJin-Gui ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23896 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shucai Wang Ying Chang Jianjun Guo Qingning Zeng Brian E Ellis Jin-Gui Chen Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
description	<h4>Background</h4>The Arabidopsis genome contains 18 genes that are predicted to encode Ovate Family Proteins (AtOFPs), a protein family characterized by a conserved OVATE domain, an approximately 70-amino acid domain that was originally found in tomato OVATE protein. Among AtOFP family members, AtOFP1 has been shown to suppress cell elongation, in part, by suppressing the expression of AtGA20ox1, AtOFP4 has been shown to regulate secondary cell wall formation by interact with KNOTTED1-LIKE HOMEODOMAIN PROTEIN 7 (KNAT7), and AtOFP5 has been shown to regulate the activity of a BEL1-LIKEHOMEODOMAIN 1(BLH1)-KNAT3 complex during early embryo sac development, but little is known about the function of other AtOFPs.<h4>Methodology/principal findings</h4>We demonstrated here that AtOFP proteins could function as effective transcriptional repressors in the Arabidopsis protoplast transient expression system. The analysis of loss-of-function alleles of AtOFPs suggested AtOFP genes may have overlapping function in regulating plant growth and development, because none of the single mutants identified, including T-DNA insertion mutants in AtOFP1, AtOFP4, AtOFP8, AtOFP10, AtOFP15 and AtOFP16, displayed any apparent morphological defects. Further, Atofp1 Atofp4 and Atofp15 Atofp16 double mutants still did not differ significantly from wild-type. On the other hand, plants overexpressing AtOFP genes displayed a number of abnormal phenotypes, which could be categorized into three distinct classes, suggesting that AtOFP genes may also have diverse functions in regulating plant growth and development. Further analysis suggested that AtOFP1 regulates cotyledon development in a postembryonic manner, and global transcript profiling revealed that it suppress the expression of many other genes.<h4>Conclusions/significance</h4>Our results showed that AtOFPs function as transcriptional repressors and they regulate multiple aspects of plant growth and development. These results provided the first overview of a previously unknown transcriptional repressor family, and revealed their possible roles in plant growth and development.
format	article
author	Shucai Wang Ying Chang Jianjun Guo Qingning Zeng Brian E Ellis Jin-Gui Chen
author_facet	Shucai Wang Ying Chang Jianjun Guo Qingning Zeng Brian E Ellis Jin-Gui Chen
author_sort	Shucai Wang
title	Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
title_short	Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
title_full	Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
title_fullStr	Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
title_full_unstemmed	Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
title_sort	arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/84a850af97fd475a97309e5d8eafab8f
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Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development.

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