Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)

Abstract Iron is a very important nutrient for cells; however, it could also cause fatal effects because of its capability to trigger oxidative stress. Due to high exposure to iron from their blood diet, ticks make use of several mechanisms to cope up with oxidative stress. One mechanism is iron seq...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Emmanuel Pacia Hernandez, Kodai Kusakisako, Melbourne Rio Talactac, Remil Linggatong Galay, Kentaro Yoshii, Tetsuya Tanaka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	ISE6 Cells Ferent mRNAs Ferrous Iron Concentration Indirect Immunofluorescent Antibody Test (IFAT) Ferrous Sulfate Treatment Medicine R Science Q
Acceso en línea:	https://doaj.org/article/84ac879a1f174a728afeb9df7de7de22
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:84ac879a1f174a728afeb9df7de7de22
record_format	dspace
spelling	oai:doaj.org-article:84ac879a1f174a728afeb9df7de7de222021-12-02T15:05:29ZInduction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)10.1038/s41598-018-34860-32045-2322https://doaj.org/article/84ac879a1f174a728afeb9df7de7de222018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-34860-3https://doaj.org/toc/2045-2322Abstract Iron is a very important nutrient for cells; however, it could also cause fatal effects because of its capability to trigger oxidative stress. Due to high exposure to iron from their blood diet, ticks make use of several mechanisms to cope up with oxidative stress. One mechanism is iron sequestration by ferritin and its control protein (IRP). Since the IRP activity is dependent on the ferrous iron concentration, we tried to induce intracellular ferritin (FER1) protein expression by exposing Ixodes scapularis embryo-derived cell line (ISE6) to different concentrations of ferrous sulphate at different time points. We were able to induce FER1 protein after exposure to 2 mM of ferrous sulphate for 48 h, as observed in both Western blotting and indirect immunofluorescent antibody tests. This could indicate that the FER1 produced could be a product of the release of IRPs from the FER1 mRNA leading to its translation. The RNA interference of FER1, through the transfection of dsRNA, led to an increase in mortality and decrease in the cellular proliferation of ISE6 cells. Overall, ISE6 cells could be a good tool in further understanding the mechanism of FER1 action, not just in Ixodes ticks but in other tick species as well.Emmanuel Pacia HernandezKodai KusakisakoMelbourne Rio TalactacRemil Linggatong GalayKentaro YoshiiTetsuya TanakaNature PortfolioarticleISE6 CellsFerent mRNAsFerrous Iron ConcentrationIndirect Immunofluorescent Antibody Test (IFAT)Ferrous Sulfate TreatmentMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ISE6 Cells Ferent mRNAs Ferrous Iron Concentration Indirect Immunofluorescent Antibody Test (IFAT) Ferrous Sulfate Treatment Medicine R Science Q
spellingShingle	ISE6 Cells Ferent mRNAs Ferrous Iron Concentration Indirect Immunofluorescent Antibody Test (IFAT) Ferrous Sulfate Treatment Medicine R Science Q Emmanuel Pacia Hernandez Kodai Kusakisako Melbourne Rio Talactac Remil Linggatong Galay Kentaro Yoshii Tetsuya Tanaka Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
description	Abstract Iron is a very important nutrient for cells; however, it could also cause fatal effects because of its capability to trigger oxidative stress. Due to high exposure to iron from their blood diet, ticks make use of several mechanisms to cope up with oxidative stress. One mechanism is iron sequestration by ferritin and its control protein (IRP). Since the IRP activity is dependent on the ferrous iron concentration, we tried to induce intracellular ferritin (FER1) protein expression by exposing Ixodes scapularis embryo-derived cell line (ISE6) to different concentrations of ferrous sulphate at different time points. We were able to induce FER1 protein after exposure to 2 mM of ferrous sulphate for 48 h, as observed in both Western blotting and indirect immunofluorescent antibody tests. This could indicate that the FER1 produced could be a product of the release of IRPs from the FER1 mRNA leading to its translation. The RNA interference of FER1, through the transfection of dsRNA, led to an increase in mortality and decrease in the cellular proliferation of ISE6 cells. Overall, ISE6 cells could be a good tool in further understanding the mechanism of FER1 action, not just in Ixodes ticks but in other tick species as well.
format	article
author	Emmanuel Pacia Hernandez Kodai Kusakisako Melbourne Rio Talactac Remil Linggatong Galay Kentaro Yoshii Tetsuya Tanaka
author_facet	Emmanuel Pacia Hernandez Kodai Kusakisako Melbourne Rio Talactac Remil Linggatong Galay Kentaro Yoshii Tetsuya Tanaka
author_sort	Emmanuel Pacia Hernandez
title	Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
title_short	Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
title_full	Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
title_fullStr	Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
title_full_unstemmed	Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)
title_sort	induction of intracellular ferritin expression in embryo-derived ixodes scapularis cell line (ise6)
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/84ac879a1f174a728afeb9df7de7de22
work_keys_str_mv	AT emmanuelpaciahernandez inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6 AT kodaikusakisako inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6 AT melbourneriotalactac inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6 AT remillinggatonggalay inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6 AT kentaroyoshii inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6 AT tetsuyatanaka inductionofintracellularferritinexpressioninembryoderivedixodesscapulariscelllineise6
_version_	1718388843270373376

Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6)

Ejemplares similares