Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria

This study was designed to assess the androgenic potential of 180 pepper accessions and 11 progenies (four F1 and seven BC) possessing PMMoV resistance in order to complement an ongoing pepper breeding program. The experiment was carried out in 10 replications with 20 anthers for each accession in t...

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Autores principales:	Stanislava Grozeva, Gancho Pasev, Vesela Radeva-Ivanova, Velichka Todorova, Valentina Ivanova, Amol N. Nankar
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	androgenesis pepper varietal groups Bulgarian peppers haploid: diploid regenerants PMMoV resistance capsicum double haploids Botany QK1-989
Acceso en línea:	https://doaj.org/article/84ad81b87ff04c57b19e6ef096bd39b4
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spelling	oai:doaj.org-article:84ad81b87ff04c57b19e6ef096bd39b42021-11-25T18:46:24ZDouble Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria10.3390/plants101124142223-7747https://doaj.org/article/84ad81b87ff04c57b19e6ef096bd39b42021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2414https://doaj.org/toc/2223-7747This study was designed to assess the androgenic potential of 180 pepper accessions and 11 progenies (four F1 and seven BC) possessing PMMoV resistance in order to complement an ongoing pepper breeding program. The experiment was carried out in 10 replications with 20 anthers for each accession in two different induction mediums from 2017 to 2019. The highest androgenic response was observed in culture medium 17-2 but differences between two mediums were nonsignificant. From a total of 191 genotypes, 102 genotypes expressed a potential for direct embryogenesis. Embryo induction was seen to be genotype-dependent and decreased in the following order: Pumpkin > Conical > Bell or blocky > Round > Elongate as the most responsive genotypes with over 10% reacted anthers being observed in CAPS-23, CAPS-29, CAPS-127, CAPS-157, CAPS-169, F1 and BC 887 derived from CAPS-23. The number of regenerated plants was higher in the conical group and least in the round varietal group. Regenerated plants were examined visually and by flow cytometry for identification of spontaneous doubled haploids (DH) and haploids. Those originating from F1 and BC progenies were additionally evaluated by a CAPS marker targeting L4 allele for resistance against PMMoV. Obtained results revealed two groups consisting of homozygous susceptible and resistant plants. Therefore, use of anther culture in ongoing breeding will greatly facilitate the pepper genetic improvement.Stanislava GrozevaGancho PasevVesela Radeva-IvanovaVelichka TodorovaValentina IvanovaAmol N. NankarMDPI AGarticleandrogenesispepper varietal groupsBulgarian peppershaploid: diploid regenerantsPMMoV resistancecapsicum double haploidsBotanyQK1-989ENPlants, Vol 10, Iss 2414, p 2414 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	androgenesis pepper varietal groups Bulgarian peppers haploid: diploid regenerants PMMoV resistance capsicum double haploids Botany QK1-989
spellingShingle	androgenesis pepper varietal groups Bulgarian peppers haploid: diploid regenerants PMMoV resistance capsicum double haploids Botany QK1-989 Stanislava Grozeva Gancho Pasev Vesela Radeva-Ivanova Velichka Todorova Valentina Ivanova Amol N. Nankar Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
description	This study was designed to assess the androgenic potential of 180 pepper accessions and 11 progenies (four F1 and seven BC) possessing PMMoV resistance in order to complement an ongoing pepper breeding program. The experiment was carried out in 10 replications with 20 anthers for each accession in two different induction mediums from 2017 to 2019. The highest androgenic response was observed in culture medium 17-2 but differences between two mediums were nonsignificant. From a total of 191 genotypes, 102 genotypes expressed a potential for direct embryogenesis. Embryo induction was seen to be genotype-dependent and decreased in the following order: Pumpkin > Conical > Bell or blocky > Round > Elongate as the most responsive genotypes with over 10% reacted anthers being observed in CAPS-23, CAPS-29, CAPS-127, CAPS-157, CAPS-169, F1 and BC 887 derived from CAPS-23. The number of regenerated plants was higher in the conical group and least in the round varietal group. Regenerated plants were examined visually and by flow cytometry for identification of spontaneous doubled haploids (DH) and haploids. Those originating from F1 and BC progenies were additionally evaluated by a CAPS marker targeting L4 allele for resistance against PMMoV. Obtained results revealed two groups consisting of homozygous susceptible and resistant plants. Therefore, use of anther culture in ongoing breeding will greatly facilitate the pepper genetic improvement.
format	article
author	Stanislava Grozeva Gancho Pasev Vesela Radeva-Ivanova Velichka Todorova Valentina Ivanova Amol N. Nankar
author_facet	Stanislava Grozeva Gancho Pasev Vesela Radeva-Ivanova Velichka Todorova Valentina Ivanova Amol N. Nankar
author_sort	Stanislava Grozeva
title	Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
title_short	Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
title_full	Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
title_fullStr	Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
title_full_unstemmed	Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria
title_sort	double haploid development and assessment of androgenic competence of balkan pepper core collection in bulgaria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/84ad81b87ff04c57b19e6ef096bd39b4
work_keys_str_mv	AT stanislavagrozeva doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria AT ganchopasev doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria AT veselaradevaivanova doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria AT velichkatodorova doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria AT valentinaivanova doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria AT amolnnankar doublehaploiddevelopmentandassessmentofandrogeniccompetenceofbalkanpeppercorecollectioninbulgaria
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Double Haploid Development and Assessment of Androgenic Competence of Balkan Pepper Core Collection in Bulgaria

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